Dynamics of the Metabolome of <i>Aliinostoc</i> sp. PMC 882.14 in Response to Light and Temperature Variations

Cyanobacteria are microorganisms able to adapt to a wide variety of environmental conditions and abiotic stresses. They produce a large number of metabolites that can participate in the dynamic adaptation of cyanobacteria to a range of different light, temperature, and nutrient conditions. Studying...

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Autores principales:	Damien Le Moigne, Justine Demay, Anita Reinhardt, Cécile Bernard, Sandra Kim Tiam, Benjamin Marie
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cyanobacteria metabolomics high-resolution mass spectrometry secondary metabolite induction culture conditions Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/3b6f52a5d9fa4365b5a1d2fe8f418ac9
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spelling	oai:doaj.org-article:3b6f52a5d9fa4365b5a1d2fe8f418ac92021-11-25T18:20:36ZDynamics of the Metabolome of <i>Aliinostoc</i> sp. PMC 882.14 in Response to Light and Temperature Variations10.3390/metabo111107452218-1989https://doaj.org/article/3b6f52a5d9fa4365b5a1d2fe8f418ac92021-10-01T00:00:00Zhttps://www.mdpi.com/2218-1989/11/11/745https://doaj.org/toc/2218-1989Cyanobacteria are microorganisms able to adapt to a wide variety of environmental conditions and abiotic stresses. They produce a large number of metabolites that can participate in the dynamic adaptation of cyanobacteria to a range of different light, temperature, and nutrient conditions. Studying the metabolite profile is one way to understand how the physiological status of cells is related to their adaptive response. In this study, we sought to understand how the diversity and dynamics of the whole metabolome depended on the growth phase and various abiotic factors such as light intensity and temperature. The cyanobacterium, <i>Aliinostoc</i> sp. PMC 882.14, was selected for its large number of biosynthetic gene clusters. One group of cells was grown under normal conditions as a control, while other groups were grown under higher light or temperature. Metabolomes were analyzed by mass spectrometry (qTOF-MS/MS) combined with untargeted analysis to investigate metabolite dynamics, and significant variation was found between exponential and stationary phases, regardless of culture conditions. In the higher light group, the synthesis of several metabolites, including shinorine, was induced while other metabolites, such as microviridins, were synthesized under higher temperature conditions. Among highly regulated metabolites, we observed the presence of mycosporine-like amino acids (MAAs) and variants of somamides, microginins, and microviridins. This study demonstrated the importance of considering the physiological state of cyanobacteria for comparative global metabolomics and studies of the regulatory processes involved in production of specific metabolites. Our results also open up new perspectives on the use of organisms such as cyanobacteria for the targeted production of bioactive metabolites.Damien Le MoigneJustine DemayAnita ReinhardtCécile BernardSandra Kim TiamBenjamin MarieMDPI AGarticlecyanobacteriametabolomicshigh-resolution mass spectrometrysecondary metabolite inductionculture conditionsMicrobiologyQR1-502ENMetabolites, Vol 11, Iss 745, p 745 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cyanobacteria metabolomics high-resolution mass spectrometry secondary metabolite induction culture conditions Microbiology QR1-502
spellingShingle	cyanobacteria metabolomics high-resolution mass spectrometry secondary metabolite induction culture conditions Microbiology QR1-502 Damien Le Moigne Justine Demay Anita Reinhardt Cécile Bernard Sandra Kim Tiam Benjamin Marie Dynamics of the Metabolome of <i>Aliinostoc</i> sp. PMC 882.14 in Response to Light and Temperature Variations
description	Cyanobacteria are microorganisms able to adapt to a wide variety of environmental conditions and abiotic stresses. They produce a large number of metabolites that can participate in the dynamic adaptation of cyanobacteria to a range of different light, temperature, and nutrient conditions. Studying the metabolite profile is one way to understand how the physiological status of cells is related to their adaptive response. In this study, we sought to understand how the diversity and dynamics of the whole metabolome depended on the growth phase and various abiotic factors such as light intensity and temperature. The cyanobacterium, <i>Aliinostoc</i> sp. PMC 882.14, was selected for its large number of biosynthetic gene clusters. One group of cells was grown under normal conditions as a control, while other groups were grown under higher light or temperature. Metabolomes were analyzed by mass spectrometry (qTOF-MS/MS) combined with untargeted analysis to investigate metabolite dynamics, and significant variation was found between exponential and stationary phases, regardless of culture conditions. In the higher light group, the synthesis of several metabolites, including shinorine, was induced while other metabolites, such as microviridins, were synthesized under higher temperature conditions. Among highly regulated metabolites, we observed the presence of mycosporine-like amino acids (MAAs) and variants of somamides, microginins, and microviridins. This study demonstrated the importance of considering the physiological state of cyanobacteria for comparative global metabolomics and studies of the regulatory processes involved in production of specific metabolites. Our results also open up new perspectives on the use of organisms such as cyanobacteria for the targeted production of bioactive metabolites.
format	article
author	Damien Le Moigne Justine Demay Anita Reinhardt Cécile Bernard Sandra Kim Tiam Benjamin Marie
author_facet	Damien Le Moigne Justine Demay Anita Reinhardt Cécile Bernard Sandra Kim Tiam Benjamin Marie
author_sort	Damien Le Moigne
title	Dynamics of the Metabolome of <i>Aliinostoc</i> sp. PMC 882.14 in Response to Light and Temperature Variations
title_short	Dynamics of the Metabolome of <i>Aliinostoc</i> sp. PMC 882.14 in Response to Light and Temperature Variations
title_full	Dynamics of the Metabolome of <i>Aliinostoc</i> sp. PMC 882.14 in Response to Light and Temperature Variations
title_fullStr	Dynamics of the Metabolome of <i>Aliinostoc</i> sp. PMC 882.14 in Response to Light and Temperature Variations
title_full_unstemmed	Dynamics of the Metabolome of <i>Aliinostoc</i> sp. PMC 882.14 in Response to Light and Temperature Variations
title_sort	dynamics of the metabolome of <i>aliinostoc</i> sp. pmc 882.14 in response to light and temperature variations
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3b6f52a5d9fa4365b5a1d2fe8f418ac9
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Dynamics of the Metabolome of <i>Aliinostoc</i> sp. PMC 882.14 in Response to Light and Temperature Variations

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