Novel approaches to circumvent the devastating effects of pests on sugarcane

Abstract Sugarcane (Saccharum officinarum L.) is a cash crop grown commercially for its higher amounts of sucrose, stored within the mature internodes of the stem. Numerous studies have been done for the resistance development against biotic and abiotic stresses to save the sucrose yields. Quality a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zahida Qamar, Idrees Ahmad Nasir, Mounir G. Abouhaidar, Kathleen L. Hefferon, Abdul Qayyum Rao, Ayesha Latif, Qurban Ali, Saima Anwar, Bushra Rashid, Ahmad Ali Shahid
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3b7316ad4c7b4347ab7af880b3ef4f69
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3b7316ad4c7b4347ab7af880b3ef4f69
record_format	dspace
spelling	oai:doaj.org-article:3b7316ad4c7b4347ab7af880b3ef4f692021-12-02T17:40:48ZNovel approaches to circumvent the devastating effects of pests on sugarcane10.1038/s41598-021-91985-82045-2322https://doaj.org/article/3b7316ad4c7b4347ab7af880b3ef4f692021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91985-8https://doaj.org/toc/2045-2322Abstract Sugarcane (Saccharum officinarum L.) is a cash crop grown commercially for its higher amounts of sucrose, stored within the mature internodes of the stem. Numerous studies have been done for the resistance development against biotic and abiotic stresses to save the sucrose yields. Quality and yield of sugarcane production is always threatened by the damages of cane borers and weeds. In current study two problems were better addressed through the genetic modification of sugarcane for provision of resistance against insects and weedicide via the expression of two modified cane borer resistant CEMB-Cry1Ac (1.8 kb), CEMB-Cry2A (1.9 kb) and one glyphosate tolerant CEMB-GTGene (1.4 kb) genes, driven by maize Ubiquitin Promoter and nos terminator. Insect Bio-toxicity assays were carried out for the assessment of Cry proteins through mortality percent of shoot borer Chilo infuscatellus at 2nd instar larvae stage. During V0, V1 and V2 generations young leaves from the transgenic sugarcane plants were collected at plant age of 20, 40, 60, 80 days and fed to the Chilo infuscatellus larvae. Up to 100% mortality of Chilo infuscatellus from 80 days old transgenic plants of V2 generation indicated that these transgenic plants were highly resistant against shoot borer and the gene expression level is sufficient to provide complete resistance against target pests. Glyphosate spray assay was carried out for complete removal of weeds. In V1-generation, 70–76% transgenic sugarcane plants were found tolerant against glyphosate spray (3000 mL/ha) under field conditions. While in V2-generation, the replicates of five selected lines 4L/2, 5L/5, 6L/5, L8/4, and L9/6 were found 100% tolerant against 3000 mL/ha glyphosate spray. It is evident from current study that CEMB-GTGene, CEMB-Cry1Ac and CEMB-Cry2A genes expression in sugarcane variety CPF-246 showed an efficient resistance against cane borers (Chilo infuscatellus) and was also highly tolerant against glyphosate spray. The selected transgenic sugarcane lines showed sustainable resistance against cane borer and glyphosate spray can be further exploited at farmer’s field level after fulfilling the biosafety requirements to boost the sugarcane production in the country.Zahida QamarIdrees Ahmad NasirMounir G. AbouhaidarKathleen L. HefferonAbdul Qayyum RaoAyesha LatifQurban AliSaima AnwarBushra RashidAhmad Ali ShahidNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-25 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zahida Qamar Idrees Ahmad Nasir Mounir G. Abouhaidar Kathleen L. Hefferon Abdul Qayyum Rao Ayesha Latif Qurban Ali Saima Anwar Bushra Rashid Ahmad Ali Shahid Novel approaches to circumvent the devastating effects of pests on sugarcane
description	Abstract Sugarcane (Saccharum officinarum L.) is a cash crop grown commercially for its higher amounts of sucrose, stored within the mature internodes of the stem. Numerous studies have been done for the resistance development against biotic and abiotic stresses to save the sucrose yields. Quality and yield of sugarcane production is always threatened by the damages of cane borers and weeds. In current study two problems were better addressed through the genetic modification of sugarcane for provision of resistance against insects and weedicide via the expression of two modified cane borer resistant CEMB-Cry1Ac (1.8 kb), CEMB-Cry2A (1.9 kb) and one glyphosate tolerant CEMB-GTGene (1.4 kb) genes, driven by maize Ubiquitin Promoter and nos terminator. Insect Bio-toxicity assays were carried out for the assessment of Cry proteins through mortality percent of shoot borer Chilo infuscatellus at 2nd instar larvae stage. During V0, V1 and V2 generations young leaves from the transgenic sugarcane plants were collected at plant age of 20, 40, 60, 80 days and fed to the Chilo infuscatellus larvae. Up to 100% mortality of Chilo infuscatellus from 80 days old transgenic plants of V2 generation indicated that these transgenic plants were highly resistant against shoot borer and the gene expression level is sufficient to provide complete resistance against target pests. Glyphosate spray assay was carried out for complete removal of weeds. In V1-generation, 70–76% transgenic sugarcane plants were found tolerant against glyphosate spray (3000 mL/ha) under field conditions. While in V2-generation, the replicates of five selected lines 4L/2, 5L/5, 6L/5, L8/4, and L9/6 were found 100% tolerant against 3000 mL/ha glyphosate spray. It is evident from current study that CEMB-GTGene, CEMB-Cry1Ac and CEMB-Cry2A genes expression in sugarcane variety CPF-246 showed an efficient resistance against cane borers (Chilo infuscatellus) and was also highly tolerant against glyphosate spray. The selected transgenic sugarcane lines showed sustainable resistance against cane borer and glyphosate spray can be further exploited at farmer’s field level after fulfilling the biosafety requirements to boost the sugarcane production in the country.
format	article
author	Zahida Qamar Idrees Ahmad Nasir Mounir G. Abouhaidar Kathleen L. Hefferon Abdul Qayyum Rao Ayesha Latif Qurban Ali Saima Anwar Bushra Rashid Ahmad Ali Shahid
author_facet	Zahida Qamar Idrees Ahmad Nasir Mounir G. Abouhaidar Kathleen L. Hefferon Abdul Qayyum Rao Ayesha Latif Qurban Ali Saima Anwar Bushra Rashid Ahmad Ali Shahid
author_sort	Zahida Qamar
title	Novel approaches to circumvent the devastating effects of pests on sugarcane
title_short	Novel approaches to circumvent the devastating effects of pests on sugarcane
title_full	Novel approaches to circumvent the devastating effects of pests on sugarcane
title_fullStr	Novel approaches to circumvent the devastating effects of pests on sugarcane
title_full_unstemmed	Novel approaches to circumvent the devastating effects of pests on sugarcane
title_sort	novel approaches to circumvent the devastating effects of pests on sugarcane
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3b7316ad4c7b4347ab7af880b3ef4f69
work_keys_str_mv	AT zahidaqamar novelapproachestocircumventthedevastatingeffectsofpestsonsugarcane AT idreesahmadnasir novelapproachestocircumventthedevastatingeffectsofpestsonsugarcane AT mounirgabouhaidar novelapproachestocircumventthedevastatingeffectsofpestsonsugarcane AT kathleenlhefferon novelapproachestocircumventthedevastatingeffectsofpestsonsugarcane AT abdulqayyumrao novelapproachestocircumventthedevastatingeffectsofpestsonsugarcane AT ayeshalatif novelapproachestocircumventthedevastatingeffectsofpestsonsugarcane AT qurbanali novelapproachestocircumventthedevastatingeffectsofpestsonsugarcane AT saimaanwar novelapproachestocircumventthedevastatingeffectsofpestsonsugarcane AT bushrarashid novelapproachestocircumventthedevastatingeffectsofpestsonsugarcane AT ahmadalishahid novelapproachestocircumventthedevastatingeffectsofpestsonsugarcane
_version_	1718379697915559936

Novel approaches to circumvent the devastating effects of pests on sugarcane

Ejemplares similares