The SARS-CoV-2 nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein

The SARS-CoV-2 nucleocapsid (N) protein binds the viral RNA genome and contains two ordered domains flanked by three intrinsically-disordered regions. Here, the authors show that RNA binding induces liquid-liquid phase separation of N, which is driven by its central intrinsically-disordered region a...

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Autores principales:	Shan Lu, Qiaozhen Ye, Digvijay Singh, Yong Cao, Jolene K. Diedrich, John R. Yates, Elizabeth Villa, Don W. Cleveland, Kevin D. Corbett
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/3b746ca51c02440b86972614f0f986c1
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spelling	oai:doaj.org-article:3b746ca51c02440b86972614f0f986c12021-12-02T10:49:32ZThe SARS-CoV-2 nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein10.1038/s41467-020-20768-y2041-1723https://doaj.org/article/3b746ca51c02440b86972614f0f986c12021-01-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-20768-yhttps://doaj.org/toc/2041-1723The SARS-CoV-2 nucleocapsid (N) protein binds the viral RNA genome and contains two ordered domains flanked by three intrinsically-disordered regions. Here, the authors show that RNA binding induces liquid-liquid phase separation of N, which is driven by its central intrinsically-disordered region and is modulated by phosphorylation. The SARS-CoV-2 Membrane (M) protein also phase-separates with N, and three-component mixtures of N + M + RNA form mutually exclusive compartments containing N + M or N + RNA.Shan LuQiaozhen YeDigvijay SinghYong CaoJolene K. DiedrichJohn R. YatesElizabeth VillaDon W. ClevelandKevin D. CorbettNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Shan Lu Qiaozhen Ye Digvijay Singh Yong Cao Jolene K. Diedrich John R. Yates Elizabeth Villa Don W. Cleveland Kevin D. Corbett The SARS-CoV-2 nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein
description	The SARS-CoV-2 nucleocapsid (N) protein binds the viral RNA genome and contains two ordered domains flanked by three intrinsically-disordered regions. Here, the authors show that RNA binding induces liquid-liquid phase separation of N, which is driven by its central intrinsically-disordered region and is modulated by phosphorylation. The SARS-CoV-2 Membrane (M) protein also phase-separates with N, and three-component mixtures of N + M + RNA form mutually exclusive compartments containing N + M or N + RNA.
format	article
author	Shan Lu Qiaozhen Ye Digvijay Singh Yong Cao Jolene K. Diedrich John R. Yates Elizabeth Villa Don W. Cleveland Kevin D. Corbett
author_facet	Shan Lu Qiaozhen Ye Digvijay Singh Yong Cao Jolene K. Diedrich John R. Yates Elizabeth Villa Don W. Cleveland Kevin D. Corbett
author_sort	Shan Lu
title	The SARS-CoV-2 nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein
title_short	The SARS-CoV-2 nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein
title_full	The SARS-CoV-2 nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein
title_fullStr	The SARS-CoV-2 nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein
title_full_unstemmed	The SARS-CoV-2 nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein
title_sort	sars-cov-2 nucleocapsid phosphoprotein forms mutually exclusive condensates with rna and the membrane-associated m protein
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3b746ca51c02440b86972614f0f986c1
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The SARS-CoV-2 nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein

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