Pseudomonas Isolation and Identification: An Introduction to the Challenges of Polyphasic Taxonomy

The ability to isolate an organism in pure culture from the environment is a manageable task for undergraduate students; the identification of that organism requires integration of both genotypic and phenotypic data and illustrates the challenges inherent in contemporary bacterial taxonomy. In this...

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Autores principales:	Kathleen Sandman, Christopher Ecker
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2014
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3b757ab42163455ca6e6a9ab456c9696
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spelling	oai:doaj.org-article:3b757ab42163455ca6e6a9ab456c96962021-11-15T15:15:37ZPseudomonas Isolation and Identification: An Introduction to the Challenges of Polyphasic Taxonomy10.1128/jmbe.v15i2.7541935-78851935-7877https://doaj.org/article/3b757ab42163455ca6e6a9ab456c96962014-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v15i2.754https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885The ability to isolate an organism in pure culture from the environment is a manageable task for undergraduate students; the identification of that organism requires integration of both genotypic and phenotypic data and illustrates the challenges inherent in contemporary bacterial taxonomy. In this ten-laboratory period series of exercises, students isolate a strain of Pseudomonas from soil and characterize its biochemical and physiological properties, as well as determine the DNA sequence of its 16S rRNA genes. Integrating these data positions students to defend their classification of the isolate as a new species or as a member of a validly described species. Assessment data demonstrate that both knowledge of and confidence in understanding of the principles of laboratory handling of Pseudomonas and bacterial taxonomy increased following the exercises.Kathleen SandmanChristopher EckerAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 15, Iss 2, Pp 287-291 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Kathleen Sandman Christopher Ecker Pseudomonas Isolation and Identification: An Introduction to the Challenges of Polyphasic Taxonomy
description	The ability to isolate an organism in pure culture from the environment is a manageable task for undergraduate students; the identification of that organism requires integration of both genotypic and phenotypic data and illustrates the challenges inherent in contemporary bacterial taxonomy. In this ten-laboratory period series of exercises, students isolate a strain of Pseudomonas from soil and characterize its biochemical and physiological properties, as well as determine the DNA sequence of its 16S rRNA genes. Integrating these data positions students to defend their classification of the isolate as a new species or as a member of a validly described species. Assessment data demonstrate that both knowledge of and confidence in understanding of the principles of laboratory handling of Pseudomonas and bacterial taxonomy increased following the exercises.
format	article
author	Kathleen Sandman Christopher Ecker
author_facet	Kathleen Sandman Christopher Ecker
author_sort	Kathleen Sandman
title	Pseudomonas Isolation and Identification: An Introduction to the Challenges of Polyphasic Taxonomy
title_short	Pseudomonas Isolation and Identification: An Introduction to the Challenges of Polyphasic Taxonomy
title_full	Pseudomonas Isolation and Identification: An Introduction to the Challenges of Polyphasic Taxonomy
title_fullStr	Pseudomonas Isolation and Identification: An Introduction to the Challenges of Polyphasic Taxonomy
title_full_unstemmed	Pseudomonas Isolation and Identification: An Introduction to the Challenges of Polyphasic Taxonomy
title_sort	pseudomonas isolation and identification: an introduction to the challenges of polyphasic taxonomy
publisher	American Society for Microbiology
publishDate	2014
url	https://doaj.org/article/3b757ab42163455ca6e6a9ab456c9696
work_keys_str_mv	AT kathleensandman pseudomonasisolationandidentificationanintroductiontothechallengesofpolyphasictaxonomy AT christopherecker pseudomonasisolationandidentificationanintroductiontothechallengesofpolyphasictaxonomy
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Pseudomonas Isolation and Identification: An Introduction to the Challenges of Polyphasic Taxonomy

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