Excitatory and inhibitory receptors utilize distinct post- and trans-synaptic mechanisms in vivo

Ionotropic neurotransmitter receptors at postsynapses mediate fast synaptic transmission upon binding of the neurotransmitter. Post- and trans-synaptic mechanisms through cytosolic, membrane, and secreted proteins have been proposed to localize neurotransmitter receptors at postsynapses. However, it...

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Autores principales:	Taisuke Miyazaki, Megumi Morimoto-Tomita, Coralie Berthoux, Kotaro Konno, Yoav Noam, Tokiwa Yamasaki, Matthijs Verhage, Pablo E Castillo, Masahiko Watanabe, Susumu Tomita
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	inhibitory excitatory receptor cell elimination in vivo postsynaptic Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3b79b81fc19f4bc9932739ee9bebb0d2
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spelling	oai:doaj.org-article:3b79b81fc19f4bc9932739ee9bebb0d22021-11-23T13:02:43ZExcitatory and inhibitory receptors utilize distinct post- and trans-synaptic mechanisms in vivo10.7554/eLife.596132050-084Xe59613https://doaj.org/article/3b79b81fc19f4bc9932739ee9bebb0d22021-10-01T00:00:00Zhttps://elifesciences.org/articles/59613https://doaj.org/toc/2050-084XIonotropic neurotransmitter receptors at postsynapses mediate fast synaptic transmission upon binding of the neurotransmitter. Post- and trans-synaptic mechanisms through cytosolic, membrane, and secreted proteins have been proposed to localize neurotransmitter receptors at postsynapses. However, it remains unknown which mechanism is crucial to maintain neurotransmitter receptors at postsynapses. In this study, we ablated excitatory or inhibitory neurons in adult mouse brains in a cell-autonomous manner. Unexpectedly, we found that excitatory AMPA receptors remain at the postsynaptic density upon ablation of excitatory presynaptic terminals. In contrast, inhibitory GABAA receptors required inhibitory presynaptic terminals for their postsynaptic localization. Consistent with this finding, ectopic expression at excitatory presynapses of neurexin-3 alpha, a putative trans-synaptic interactor with the native GABAA receptor complex, could recruit GABAA receptors to contacted postsynaptic sites. These results establish distinct mechanisms for the maintenance of excitatory and inhibitory postsynaptic receptors in the mature mammalian brain.Taisuke MiyazakiMegumi Morimoto-TomitaCoralie BerthouxKotaro KonnoYoav NoamTokiwa YamasakiMatthijs VerhagePablo E CastilloMasahiko WatanabeSusumu TomitaeLife Sciences Publications Ltdarticleinhibitoryexcitatoryreceptorcell eliminationin vivopostsynapticMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	inhibitory excitatory receptor cell elimination in vivo postsynaptic Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	inhibitory excitatory receptor cell elimination in vivo postsynaptic Medicine R Science Q Biology (General) QH301-705.5 Taisuke Miyazaki Megumi Morimoto-Tomita Coralie Berthoux Kotaro Konno Yoav Noam Tokiwa Yamasaki Matthijs Verhage Pablo E Castillo Masahiko Watanabe Susumu Tomita Excitatory and inhibitory receptors utilize distinct post- and trans-synaptic mechanisms in vivo
description	Ionotropic neurotransmitter receptors at postsynapses mediate fast synaptic transmission upon binding of the neurotransmitter. Post- and trans-synaptic mechanisms through cytosolic, membrane, and secreted proteins have been proposed to localize neurotransmitter receptors at postsynapses. However, it remains unknown which mechanism is crucial to maintain neurotransmitter receptors at postsynapses. In this study, we ablated excitatory or inhibitory neurons in adult mouse brains in a cell-autonomous manner. Unexpectedly, we found that excitatory AMPA receptors remain at the postsynaptic density upon ablation of excitatory presynaptic terminals. In contrast, inhibitory GABAA receptors required inhibitory presynaptic terminals for their postsynaptic localization. Consistent with this finding, ectopic expression at excitatory presynapses of neurexin-3 alpha, a putative trans-synaptic interactor with the native GABAA receptor complex, could recruit GABAA receptors to contacted postsynaptic sites. These results establish distinct mechanisms for the maintenance of excitatory and inhibitory postsynaptic receptors in the mature mammalian brain.
format	article
author	Taisuke Miyazaki Megumi Morimoto-Tomita Coralie Berthoux Kotaro Konno Yoav Noam Tokiwa Yamasaki Matthijs Verhage Pablo E Castillo Masahiko Watanabe Susumu Tomita
author_facet	Taisuke Miyazaki Megumi Morimoto-Tomita Coralie Berthoux Kotaro Konno Yoav Noam Tokiwa Yamasaki Matthijs Verhage Pablo E Castillo Masahiko Watanabe Susumu Tomita
author_sort	Taisuke Miyazaki
title	Excitatory and inhibitory receptors utilize distinct post- and trans-synaptic mechanisms in vivo
title_short	Excitatory and inhibitory receptors utilize distinct post- and trans-synaptic mechanisms in vivo
title_full	Excitatory and inhibitory receptors utilize distinct post- and trans-synaptic mechanisms in vivo
title_fullStr	Excitatory and inhibitory receptors utilize distinct post- and trans-synaptic mechanisms in vivo
title_full_unstemmed	Excitatory and inhibitory receptors utilize distinct post- and trans-synaptic mechanisms in vivo
title_sort	excitatory and inhibitory receptors utilize distinct post- and trans-synaptic mechanisms in vivo
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/3b79b81fc19f4bc9932739ee9bebb0d2
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Excitatory and inhibitory receptors utilize distinct post- and trans-synaptic mechanisms in vivo

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