Apolipoprotein E3-mediated cellular uptake of reconstituted high-density lipoprotein bearing core 3, 10, or 17 nm hydrophobic gold nanoparticles

Skylar T Chuang, Young-Seok Shon, Vasanthy Narayanaswami Department of Chemistry and Biochemistry, California State University Long Beach, Long Beach, CA, USA Abstract: We have developed a high-density lipoprotein (HDL)-based platform for transport and delivery of hydrophobic gold nanoparticles (A...

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Autores principales:	Chuang ST, Shon YS, Narayanaswami V
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Apolipoprotein E Gold nanoparticles Lipoproteins HDL Cancer Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/3b7a35de0c954501a60c3519ce1f02e3
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spelling	oai:doaj.org-article:3b7a35de0c954501a60c3519ce1f02e32021-12-02T01:46:43ZApolipoprotein E3-mediated cellular uptake of reconstituted high-density lipoprotein bearing core 3, 10, or 17 nm hydrophobic gold nanoparticles1178-2013https://doaj.org/article/3b7a35de0c954501a60c3519ce1f02e32017-11-01T00:00:00Zhttps://www.dovepress.com/apolipoprotein-e3-mediated-cellular-uptake-of-reconstituted-high-densi-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Skylar T Chuang, Young-Seok Shon, Vasanthy Narayanaswami Department of Chemistry and Biochemistry, California State University Long Beach, Long Beach, CA, USA Abstract: We have developed a high-density lipoprotein (HDL)-based platform for transport and delivery of hydrophobic gold nanoparticles (AuNPs). The ability of apolipoprotein E3 (apoE3) to act as a high-affinity ligand for the low-density lipoprotein receptor (LDLr) was exploited to gain entry of HDL with AuNPs into glioblastoma cells. AuNPs of 3, 10, and 17 nm diameter, the latter two synthesized by phase transfer process, were solubilized by integration with phospholipids and apoE3, yielding reconstituted HDL (rHDL) bearing AuNPs. Ultraviolet–visible spectra of rHDL-AuNP indicated the presence of stable particles with surface plasmon band at ~530 nm. Transmission electron microscopy (TEM) of rHDL-AuNP revealed roughly spherical particles with AuNPs embedded in the core. The rHDL-AuNP particles displayed robust binding to the LDLr and were internalized by receptor-mediated endocytosis in glioblastoma cells. Confocal microscopy confirmed cellular uptake of AuNPs in the endosomal–lysosomal compartments, while TEM revealed intracellular aggregated AuNPs. Cell viability assay demonstrated that >85% of cells were viable with rHDL-AuNP treatment of 0.1–100 µg/mL for 24 hours. These findings are significant since they offer an effective means of delivering AuNPs across the cell membrane, which is particularly relevant in tumor cells that overexpress LDLr. Keywords: apolipoprotein E, gold nanoparticles, lipoproteins, HDL, cancerChuang STShon YSNarayanaswami VDove Medical PressarticleApolipoprotein EGold nanoparticlesLipoproteinsHDLCancerMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 8495-8510 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Apolipoprotein E Gold nanoparticles Lipoproteins HDL Cancer Medicine (General) R5-920
spellingShingle	Apolipoprotein E Gold nanoparticles Lipoproteins HDL Cancer Medicine (General) R5-920 Chuang ST Shon YS Narayanaswami V Apolipoprotein E3-mediated cellular uptake of reconstituted high-density lipoprotein bearing core 3, 10, or 17 nm hydrophobic gold nanoparticles
description	Skylar T Chuang, Young-Seok Shon, Vasanthy Narayanaswami Department of Chemistry and Biochemistry, California State University Long Beach, Long Beach, CA, USA Abstract: We have developed a high-density lipoprotein (HDL)-based platform for transport and delivery of hydrophobic gold nanoparticles (AuNPs). The ability of apolipoprotein E3 (apoE3) to act as a high-affinity ligand for the low-density lipoprotein receptor (LDLr) was exploited to gain entry of HDL with AuNPs into glioblastoma cells. AuNPs of 3, 10, and 17 nm diameter, the latter two synthesized by phase transfer process, were solubilized by integration with phospholipids and apoE3, yielding reconstituted HDL (rHDL) bearing AuNPs. Ultraviolet–visible spectra of rHDL-AuNP indicated the presence of stable particles with surface plasmon band at ~530 nm. Transmission electron microscopy (TEM) of rHDL-AuNP revealed roughly spherical particles with AuNPs embedded in the core. The rHDL-AuNP particles displayed robust binding to the LDLr and were internalized by receptor-mediated endocytosis in glioblastoma cells. Confocal microscopy confirmed cellular uptake of AuNPs in the endosomal–lysosomal compartments, while TEM revealed intracellular aggregated AuNPs. Cell viability assay demonstrated that >85% of cells were viable with rHDL-AuNP treatment of 0.1–100 µg/mL for 24 hours. These findings are significant since they offer an effective means of delivering AuNPs across the cell membrane, which is particularly relevant in tumor cells that overexpress LDLr. Keywords: apolipoprotein E, gold nanoparticles, lipoproteins, HDL, cancer
format	article
author	Chuang ST Shon YS Narayanaswami V
author_facet	Chuang ST Shon YS Narayanaswami V
author_sort	Chuang ST
title	Apolipoprotein E3-mediated cellular uptake of reconstituted high-density lipoprotein bearing core 3, 10, or 17 nm hydrophobic gold nanoparticles
title_short	Apolipoprotein E3-mediated cellular uptake of reconstituted high-density lipoprotein bearing core 3, 10, or 17 nm hydrophobic gold nanoparticles
title_full	Apolipoprotein E3-mediated cellular uptake of reconstituted high-density lipoprotein bearing core 3, 10, or 17 nm hydrophobic gold nanoparticles
title_fullStr	Apolipoprotein E3-mediated cellular uptake of reconstituted high-density lipoprotein bearing core 3, 10, or 17 nm hydrophobic gold nanoparticles
title_full_unstemmed	Apolipoprotein E3-mediated cellular uptake of reconstituted high-density lipoprotein bearing core 3, 10, or 17 nm hydrophobic gold nanoparticles
title_sort	apolipoprotein e3-mediated cellular uptake of reconstituted high-density lipoprotein bearing core 3, 10, or 17 nm hydrophobic gold nanoparticles
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/3b7a35de0c954501a60c3519ce1f02e3
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_version_	1718402888491859968

Apolipoprotein E3-mediated cellular uptake of reconstituted high-density lipoprotein bearing core 3, 10, or 17 nm hydrophobic gold nanoparticles

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