Unveiling antioxidant and anti-cancer potentials of characterized Annona reticulata leaf extract in 1,2-dimethylhydrazine-induced colorectal cancer in Wistar rats

Background: Colorectal cancer (CC) is the third most common cancer in the world. Annona reticulata (AR) also known as bullock's heart, is a traditional herb. AR leaf extract was initially investigated for its anti-bacterial, anti-inflammatory, anti-malarial, anti-helminthic, anti-stress, and wo...

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Autores principales:	Nadeem Khan G, Nitesh Kumar, Abhijna Ballal R, Divya Datta, Vijetha Shenoy Belle
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Annona reticulata 1,2-Dimethylhydrazine Colorectal cancer Antioxidant Anticancer Miscellaneous systems and treatments RZ409.7-999
Acceso en línea:	https://doaj.org/article/3b7e2ccd650542f8ab74de067509c6b5
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spelling	oai:doaj.org-article:3b7e2ccd650542f8ab74de067509c6b52021-12-02T04:59:18ZUnveiling antioxidant and anti-cancer potentials of characterized Annona reticulata leaf extract in 1,2-dimethylhydrazine-induced colorectal cancer in Wistar rats0975-947610.1016/j.jaim.2021.05.010https://doaj.org/article/3b7e2ccd650542f8ab74de067509c6b52021-10-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0975947621001017https://doaj.org/toc/0975-9476Background: Colorectal cancer (CC) is the third most common cancer in the world. Annona reticulata (AR) also known as bullock's heart, is a traditional herb. AR leaf extract was initially investigated for its anti-bacterial, anti-inflammatory, anti-malarial, anti-helminthic, anti-stress, and wound healing properties. Only a few in vitro cancer studies have been conducted on AR. Although few studies have linked AR leaf extract to many cancers, comprehensive studies addressing regulation, biological functions, and molecular mechanisms leading to CC pathogenesis are clearly lacking. Objectives: The present study aimed to explore the antioxidant and anti-cancer potentials of AR leaf extract in CC. Materials and methods: The MTT assay was used to test the anti-proliferative activity of AR leaf extract in vitro on the HCT116 cell line. Qualitative and quantitative phytochemical characterization was carried out using gas chromatography: mass spectrometry (GC–MS). 1,2-dimethylhydrazine (DMH) was used to establish CC model in female Wistar rats. The acute toxicity of AR leaf extract was tested in accordance with OECD guidelines. Aberrant Crypt Foci (ACF) count, organ index, and hematological estimations were used to screen for in vivo anti-cancer potential. The antioxidant activity of colon homogenate was determined. Results: The alcoholic leaf extract (IC50, 0.55 μg/ml) was found to be more potent than the aqueous extract. Using GC–MS, a total of 108 compounds were quantified in the alcoholic leaf extract. The LD 50 value was found to be safe at a dose of 98.11 mg/kg of body weight. AR alcoholic leaf extract significantly (p < 0.05) decreased ACF count and normalized colon length/weight ratio. AR leaf extract increased RBC, hemoglobin and platelets levels. The AR alcoholic leaf extract reduced the DMH-induced tumors and significantly (p < 0.05) increased the activity of endogenous antioxidant enzymes such as catalase, reduced glutathione, superoxide dismutase, and decreased the lipid peroxidase activity. AR leaf extract reduced the inflammation caused by DMH and helped to repair the colon's damaged muscle layers. Conclusion: Based on the findings from the present study, it can be concluded that the alcoholic leaf extract of AR has antioxidant and anti-proliferative properties and can aid in the prevention of CC development and dysplasia caused by DMH.Nadeem Khan GNitesh KumarAbhijna Ballal RDivya DattaVijetha Shenoy BelleElsevierarticleAnnona reticulata1,2-DimethylhydrazineColorectal cancerAntioxidantAnticancerMiscellaneous systems and treatmentsRZ409.7-999ENJournal of Ayurveda and Integrative Medicine, Vol 12, Iss 4, Pp 579-589 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Annona reticulata 1,2-Dimethylhydrazine Colorectal cancer Antioxidant Anticancer Miscellaneous systems and treatments RZ409.7-999
spellingShingle	Annona reticulata 1,2-Dimethylhydrazine Colorectal cancer Antioxidant Anticancer Miscellaneous systems and treatments RZ409.7-999 Nadeem Khan G Nitesh Kumar Abhijna Ballal R Divya Datta Vijetha Shenoy Belle Unveiling antioxidant and anti-cancer potentials of characterized Annona reticulata leaf extract in 1,2-dimethylhydrazine-induced colorectal cancer in Wistar rats
description	Background: Colorectal cancer (CC) is the third most common cancer in the world. Annona reticulata (AR) also known as bullock's heart, is a traditional herb. AR leaf extract was initially investigated for its anti-bacterial, anti-inflammatory, anti-malarial, anti-helminthic, anti-stress, and wound healing properties. Only a few in vitro cancer studies have been conducted on AR. Although few studies have linked AR leaf extract to many cancers, comprehensive studies addressing regulation, biological functions, and molecular mechanisms leading to CC pathogenesis are clearly lacking. Objectives: The present study aimed to explore the antioxidant and anti-cancer potentials of AR leaf extract in CC. Materials and methods: The MTT assay was used to test the anti-proliferative activity of AR leaf extract in vitro on the HCT116 cell line. Qualitative and quantitative phytochemical characterization was carried out using gas chromatography: mass spectrometry (GC–MS). 1,2-dimethylhydrazine (DMH) was used to establish CC model in female Wistar rats. The acute toxicity of AR leaf extract was tested in accordance with OECD guidelines. Aberrant Crypt Foci (ACF) count, organ index, and hematological estimations were used to screen for in vivo anti-cancer potential. The antioxidant activity of colon homogenate was determined. Results: The alcoholic leaf extract (IC50, 0.55 μg/ml) was found to be more potent than the aqueous extract. Using GC–MS, a total of 108 compounds were quantified in the alcoholic leaf extract. The LD 50 value was found to be safe at a dose of 98.11 mg/kg of body weight. AR alcoholic leaf extract significantly (p < 0.05) decreased ACF count and normalized colon length/weight ratio. AR leaf extract increased RBC, hemoglobin and platelets levels. The AR alcoholic leaf extract reduced the DMH-induced tumors and significantly (p < 0.05) increased the activity of endogenous antioxidant enzymes such as catalase, reduced glutathione, superoxide dismutase, and decreased the lipid peroxidase activity. AR leaf extract reduced the inflammation caused by DMH and helped to repair the colon's damaged muscle layers. Conclusion: Based on the findings from the present study, it can be concluded that the alcoholic leaf extract of AR has antioxidant and anti-proliferative properties and can aid in the prevention of CC development and dysplasia caused by DMH.
format	article
author	Nadeem Khan G Nitesh Kumar Abhijna Ballal R Divya Datta Vijetha Shenoy Belle
author_facet	Nadeem Khan G Nitesh Kumar Abhijna Ballal R Divya Datta Vijetha Shenoy Belle
author_sort	Nadeem Khan G
title	Unveiling antioxidant and anti-cancer potentials of characterized Annona reticulata leaf extract in 1,2-dimethylhydrazine-induced colorectal cancer in Wistar rats
title_short	Unveiling antioxidant and anti-cancer potentials of characterized Annona reticulata leaf extract in 1,2-dimethylhydrazine-induced colorectal cancer in Wistar rats
title_full	Unveiling antioxidant and anti-cancer potentials of characterized Annona reticulata leaf extract in 1,2-dimethylhydrazine-induced colorectal cancer in Wistar rats
title_fullStr	Unveiling antioxidant and anti-cancer potentials of characterized Annona reticulata leaf extract in 1,2-dimethylhydrazine-induced colorectal cancer in Wistar rats
title_full_unstemmed	Unveiling antioxidant and anti-cancer potentials of characterized Annona reticulata leaf extract in 1,2-dimethylhydrazine-induced colorectal cancer in Wistar rats
title_sort	unveiling antioxidant and anti-cancer potentials of characterized annona reticulata leaf extract in 1,2-dimethylhydrazine-induced colorectal cancer in wistar rats
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/3b7e2ccd650542f8ab74de067509c6b5
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Unveiling antioxidant and anti-cancer potentials of characterized Annona reticulata leaf extract in 1,2-dimethylhydrazine-induced colorectal cancer in Wistar rats

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