Healthy and Chronic Kidney Disease (CKD) Dogs Have Differences in Serum Metabolomics and Renal Diet May Have Slowed Disease Progression

Chronic kidney disease (CKD) is highly prevalent in dogs, and metabolomics investigation has been recently introduced for a better understanding of the role of diet in CKD. This study aimed to compare the serum metabolomic profile of healthy dogs (CG) and dogs with CKD (CKD-T0 and CKD-T6) to evaluat...

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Autores principales:	Marcio Antonio Brunetto, Bruna Ruberti, Doris Pereira Halfen, Douglas Segalla Caragelasco, Thiago Henrique Annibale Vendramini, Vivian Pedrinelli, Henrique Tobaro Macedo, Juliana Toloi Jeremias, Cristiana Fonseca Ferreira Pontieri, Fernanda Maria Marins Ocampos, Luis Alberto Colnago, Marcia Mery Kogika
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	renal dogs uremic toxins metabolic profile nutrition survival Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/3b7f67872b454be5b0b7a22e079a8e05
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spelling	oai:doaj.org-article:3b7f67872b454be5b0b7a22e079a8e052021-11-25T18:20:53ZHealthy and Chronic Kidney Disease (CKD) Dogs Have Differences in Serum Metabolomics and Renal Diet May Have Slowed Disease Progression10.3390/metabo111107822218-1989https://doaj.org/article/3b7f67872b454be5b0b7a22e079a8e052021-11-01T00:00:00Zhttps://www.mdpi.com/2218-1989/11/11/782https://doaj.org/toc/2218-1989Chronic kidney disease (CKD) is highly prevalent in dogs, and metabolomics investigation has been recently introduced for a better understanding of the role of diet in CKD. This study aimed to compare the serum metabolomic profile of healthy dogs (CG) and dogs with CKD (CKD-T0 and CKD-T6) to evaluate whether the diet would affect metabolites. Six dogs (5 females; 1 male; 7.47 ± 2.31 years old) with CKD stage 3 or 4 (IRIS) were included. CG consisted of 10 healthy female dogs (5.89 ± 2.57 years old) fed a maintenance diet. Serum metabolites were analyzed by <sup>1</sup>H nuclear magnetic resonance (<sup>1</sup>H NMR) spectra. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were performed to assess differences in metabolomic profiles between groups and before (CKD-T0) and after renal diet (CKD-T6). Data analysis was performed on SIMCA-P software. Dogs with CKD showed an altered metabolic profile with increased urea, creatinine, creatine, citrate, and lipids. Lactate, branched-chain amino acids (BCAAs), and glutamine were decreased in the CKD group. However, after 6 months of diet, the metabolite profiles of CKD-T0 and CKD-T6 were similar. Metabolomics profile may be useful to evaluate and recognize metabolic dysfunction and progression of CKD, and the diet may have helped maintain and retard the progression of CKD.Marcio Antonio BrunettoBruna RubertiDoris Pereira HalfenDouglas Segalla CaragelascoThiago Henrique Annibale VendraminiVivian PedrinelliHenrique Tobaro MacedoJuliana Toloi JeremiasCristiana Fonseca Ferreira PontieriFernanda Maria Marins OcamposLuis Alberto ColnagoMarcia Mery KogikaMDPI AGarticlerenal dogsuremic toxinsmetabolic profilenutritionsurvivalMicrobiologyQR1-502ENMetabolites, Vol 11, Iss 782, p 782 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	renal dogs uremic toxins metabolic profile nutrition survival Microbiology QR1-502
spellingShingle	renal dogs uremic toxins metabolic profile nutrition survival Microbiology QR1-502 Marcio Antonio Brunetto Bruna Ruberti Doris Pereira Halfen Douglas Segalla Caragelasco Thiago Henrique Annibale Vendramini Vivian Pedrinelli Henrique Tobaro Macedo Juliana Toloi Jeremias Cristiana Fonseca Ferreira Pontieri Fernanda Maria Marins Ocampos Luis Alberto Colnago Marcia Mery Kogika Healthy and Chronic Kidney Disease (CKD) Dogs Have Differences in Serum Metabolomics and Renal Diet May Have Slowed Disease Progression
description	Chronic kidney disease (CKD) is highly prevalent in dogs, and metabolomics investigation has been recently introduced for a better understanding of the role of diet in CKD. This study aimed to compare the serum metabolomic profile of healthy dogs (CG) and dogs with CKD (CKD-T0 and CKD-T6) to evaluate whether the diet would affect metabolites. Six dogs (5 females; 1 male; 7.47 ± 2.31 years old) with CKD stage 3 or 4 (IRIS) were included. CG consisted of 10 healthy female dogs (5.89 ± 2.57 years old) fed a maintenance diet. Serum metabolites were analyzed by <sup>1</sup>H nuclear magnetic resonance (<sup>1</sup>H NMR) spectra. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were performed to assess differences in metabolomic profiles between groups and before (CKD-T0) and after renal diet (CKD-T6). Data analysis was performed on SIMCA-P software. Dogs with CKD showed an altered metabolic profile with increased urea, creatinine, creatine, citrate, and lipids. Lactate, branched-chain amino acids (BCAAs), and glutamine were decreased in the CKD group. However, after 6 months of diet, the metabolite profiles of CKD-T0 and CKD-T6 were similar. Metabolomics profile may be useful to evaluate and recognize metabolic dysfunction and progression of CKD, and the diet may have helped maintain and retard the progression of CKD.
format	article
author	Marcio Antonio Brunetto Bruna Ruberti Doris Pereira Halfen Douglas Segalla Caragelasco Thiago Henrique Annibale Vendramini Vivian Pedrinelli Henrique Tobaro Macedo Juliana Toloi Jeremias Cristiana Fonseca Ferreira Pontieri Fernanda Maria Marins Ocampos Luis Alberto Colnago Marcia Mery Kogika
author_facet	Marcio Antonio Brunetto Bruna Ruberti Doris Pereira Halfen Douglas Segalla Caragelasco Thiago Henrique Annibale Vendramini Vivian Pedrinelli Henrique Tobaro Macedo Juliana Toloi Jeremias Cristiana Fonseca Ferreira Pontieri Fernanda Maria Marins Ocampos Luis Alberto Colnago Marcia Mery Kogika
author_sort	Marcio Antonio Brunetto
title	Healthy and Chronic Kidney Disease (CKD) Dogs Have Differences in Serum Metabolomics and Renal Diet May Have Slowed Disease Progression
title_short	Healthy and Chronic Kidney Disease (CKD) Dogs Have Differences in Serum Metabolomics and Renal Diet May Have Slowed Disease Progression
title_full	Healthy and Chronic Kidney Disease (CKD) Dogs Have Differences in Serum Metabolomics and Renal Diet May Have Slowed Disease Progression
title_fullStr	Healthy and Chronic Kidney Disease (CKD) Dogs Have Differences in Serum Metabolomics and Renal Diet May Have Slowed Disease Progression
title_full_unstemmed	Healthy and Chronic Kidney Disease (CKD) Dogs Have Differences in Serum Metabolomics and Renal Diet May Have Slowed Disease Progression
title_sort	healthy and chronic kidney disease (ckd) dogs have differences in serum metabolomics and renal diet may have slowed disease progression
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3b7f67872b454be5b0b7a22e079a8e05
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Healthy and Chronic Kidney Disease (CKD) Dogs Have Differences in Serum Metabolomics and Renal Diet May Have Slowed Disease Progression

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