Effects of lipid extraction on the isotopic values of sea turtle bone collagen

Many stable isotope analysis (SIA) studies aim to track protein, which is assimilated in animal tissues from their food sources, to assess feeding ecology, movements, and ontogenetic shifts of marine animals. Lipids are known to be a potential source of bias because they are depleted in 13C compared...

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Autores principales:	L Medeiros, D da Silveira Monteiro, R Petitet, L Bugoni
Formato:	article
Lenguaje:	EN
Publicado:	Inter-Research 2015
Materias:	Biology (General) QH301-705.5 Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/3b83c27bc58641d2839d8a14a19b3f21
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spelling	oai:doaj.org-article:3b83c27bc58641d2839d8a14a19b3f212021-11-18T09:20:54ZEffects of lipid extraction on the isotopic values of sea turtle bone collagen1864-77821864-779010.3354/ab00628https://doaj.org/article/3b83c27bc58641d2839d8a14a19b3f212015-03-01T00:00:00Zhttps://www.int-res.com/abstracts/ab/v23/n3/p191-199/https://doaj.org/toc/1864-7782https://doaj.org/toc/1864-7790Many stable isotope analysis (SIA) studies aim to track protein, which is assimilated in animal tissues from their food sources, to assess feeding ecology, movements, and ontogenetic shifts of marine animals. Lipids are known to be a potential source of bias because they are depleted in 13C compared to 12C. Although lipids are usually removed before SIA, there is a lack of standardized analytical protocols for this procedure. We tested the effects of lipid extraction with 2 chemical solvents (chloroform-methanol and petroleum ether) in the humeri of the loggerhead sea turtle Caretta caretta to establish a standard protocol for processing the bone collagen of marine animals and to develop a mathematical correction. In both lipid extraction treatments, δ13C values were higher than the control, but only lipid extraction with chloroform-methanol showed significant differences. By contrast, the δ15N values were not affected by lipid extraction treatments with either solvent. The linear regression between the C:Nbulk ratio and Δ13C was not significant, which does not support the assumption that there is a predictable relationship between the C:N ratio and lipid content. Nevertheless, a significant positive relationship between Δ13C and δ13Cbulk was observed, but such a model is not recommended as a mathematical lipid correction method because the model efficiency had a negative value, which indicates that the mean value of δ13Cle is a better predictor than the model itself. These results suggest that lipid extraction should be taken into account in SIA of bone collagen tissues for accurate δ13C determination.L MedeirosD da Silveira MonteiroR PetitetL BugoniInter-ResearcharticleBiology (General)QH301-705.5MicrobiologyQR1-502ENAquatic Biology, Vol 23, Iss 3, Pp 191-199 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5 Microbiology QR1-502
spellingShingle	Biology (General) QH301-705.5 Microbiology QR1-502 L Medeiros D da Silveira Monteiro R Petitet L Bugoni Effects of lipid extraction on the isotopic values of sea turtle bone collagen
description	Many stable isotope analysis (SIA) studies aim to track protein, which is assimilated in animal tissues from their food sources, to assess feeding ecology, movements, and ontogenetic shifts of marine animals. Lipids are known to be a potential source of bias because they are depleted in 13C compared to 12C. Although lipids are usually removed before SIA, there is a lack of standardized analytical protocols for this procedure. We tested the effects of lipid extraction with 2 chemical solvents (chloroform-methanol and petroleum ether) in the humeri of the loggerhead sea turtle Caretta caretta to establish a standard protocol for processing the bone collagen of marine animals and to develop a mathematical correction. In both lipid extraction treatments, δ13C values were higher than the control, but only lipid extraction with chloroform-methanol showed significant differences. By contrast, the δ15N values were not affected by lipid extraction treatments with either solvent. The linear regression between the C:Nbulk ratio and Δ13C was not significant, which does not support the assumption that there is a predictable relationship between the C:N ratio and lipid content. Nevertheless, a significant positive relationship between Δ13C and δ13Cbulk was observed, but such a model is not recommended as a mathematical lipid correction method because the model efficiency had a negative value, which indicates that the mean value of δ13Cle is a better predictor than the model itself. These results suggest that lipid extraction should be taken into account in SIA of bone collagen tissues for accurate δ13C determination.
format	article
author	L Medeiros D da Silveira Monteiro R Petitet L Bugoni
author_facet	L Medeiros D da Silveira Monteiro R Petitet L Bugoni
author_sort	L Medeiros
title	Effects of lipid extraction on the isotopic values of sea turtle bone collagen
title_short	Effects of lipid extraction on the isotopic values of sea turtle bone collagen
title_full	Effects of lipid extraction on the isotopic values of sea turtle bone collagen
title_fullStr	Effects of lipid extraction on the isotopic values of sea turtle bone collagen
title_full_unstemmed	Effects of lipid extraction on the isotopic values of sea turtle bone collagen
title_sort	effects of lipid extraction on the isotopic values of sea turtle bone collagen
publisher	Inter-Research
publishDate	2015
url	https://doaj.org/article/3b83c27bc58641d2839d8a14a19b3f21
work_keys_str_mv	AT lmedeiros effectsoflipidextractionontheisotopicvaluesofseaturtlebonecollagen AT ddasilveiramonteiro effectsoflipidextractionontheisotopicvaluesofseaturtlebonecollagen AT rpetitet effectsoflipidextractionontheisotopicvaluesofseaturtlebonecollagen AT lbugoni effectsoflipidextractionontheisotopicvaluesofseaturtlebonecollagen
_version_	1718420897934606336

Effects of lipid extraction on the isotopic values of sea turtle bone collagen

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