Expression of tryptophan hydroxylase in gastric mucosa in symptomatic and asymptomatic Helicobacter pylori infection

Introduction Helicobacter pylori infection induces clinical symptoms in 15–20% of subjects, and the reason for this variation is still not clear. The aim of the present study is to evaluate the expression of tryptophan hydroxylase (TpH-1) in gastric mucosa of patients with symptomatic and asymptomat...

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Autores principales:	Cezary Chojnacki, Tomasz Popławski, Aleksandra Błońska, Janusz Błasiak, Marek Romanowski, Jan Chojnacki
Formato:	article
Lenguaje:	EN
Publicado:	Termedia Publishing House 2019
Materias:	helicobacter pylori gastric mucosa tryptophan hydroxylase chronic dyspepsia increased expression of tph-1 in gastric mucosa plays a role in pathogenesis of chronic dyspepsia Medicine R
Acceso en línea:	https://doaj.org/article/3b86cd94175743168cdea80dbba7adce
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spelling	oai:doaj.org-article:3b86cd94175743168cdea80dbba7adce2021-12-02T18:39:09ZExpression of tryptophan hydroxylase in gastric mucosa in symptomatic and asymptomatic Helicobacter pylori infection1734-19221896-915110.5114/aoms.2018.76928https://doaj.org/article/3b86cd94175743168cdea80dbba7adce2019-03-01T00:00:00Zhttps://www.archivesofmedicalscience.com/Expression-of-tryptophan-hydroxylase-in-gastric-mucosa-in-symptomatic-and-asymptomatic,68557,0,2.htmlhttps://doaj.org/toc/1734-1922https://doaj.org/toc/1896-9151Introduction Helicobacter pylori infection induces clinical symptoms in 15–20% of subjects, and the reason for this variation is still not clear. The aim of the present study is to evaluate the expression of tryptophan hydroxylase (TpH-1) in gastric mucosa of patients with symptomatic and asymptomatic H. pylori infection in relation to the intensity of bacterial colonization and severity of dyspeptic symptoms. Material and methods Ninety subjects (aged 35–49 years) were enrolled in the study and separated into 3 groups of 30 subjects each. Group I – healthy volunteers without H. pylori infection, group II – subjects with asymptomatic H. pylori infection, group III – H. pylori-infected patients with dyspeptic symptoms, mainly fasting and nocturnal epigastric pain. To diagnose H. pylori infection the urea breath test (UBT-13C) and histological analysis were performed. The level of mRNA expression of tryptophan hydroxylase (TpH-1) was estimated in gastric mucosa with RT-PCR. Results The expression of this enzyme in antral mucosa was 2.69 ±0.97 in group I, 2.28 ±0.69 in group II (p > 0.05) and 4.40 ±1.64 in group III (p 0.05) and 3.40 ±1.51 (p < 0.001), respectively. In group III a positive correlation was found between intensity of H. pylori colonization and TpH-1 expression as well as between TpH-1 expression and severity of dyspeptic symptoms. Conclusions Increased expression of TpH-1 in gastric mucosa plays a role in pathogenesis of chronic dyspepsia.Cezary ChojnackiTomasz PopławskiAleksandra BłońskaJanusz BłasiakMarek RomanowskiJan ChojnackiTermedia Publishing Housearticlehelicobacter pylorigastric mucosatryptophan hydroxylasechronic dyspepsiaincreased expression of tph-1 in gastric mucosa plays a role in pathogenesis of chronic dyspepsiaMedicineRENArchives of Medical Science, Vol 15, Iss 2, Pp 416-423 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	helicobacter pylori gastric mucosa tryptophan hydroxylase chronic dyspepsia increased expression of tph-1 in gastric mucosa plays a role in pathogenesis of chronic dyspepsia Medicine R
spellingShingle	helicobacter pylori gastric mucosa tryptophan hydroxylase chronic dyspepsia increased expression of tph-1 in gastric mucosa plays a role in pathogenesis of chronic dyspepsia Medicine R Cezary Chojnacki Tomasz Popławski Aleksandra Błońska Janusz Błasiak Marek Romanowski Jan Chojnacki Expression of tryptophan hydroxylase in gastric mucosa in symptomatic and asymptomatic Helicobacter pylori infection
description	Introduction Helicobacter pylori infection induces clinical symptoms in 15–20% of subjects, and the reason for this variation is still not clear. The aim of the present study is to evaluate the expression of tryptophan hydroxylase (TpH-1) in gastric mucosa of patients with symptomatic and asymptomatic H. pylori infection in relation to the intensity of bacterial colonization and severity of dyspeptic symptoms. Material and methods Ninety subjects (aged 35–49 years) were enrolled in the study and separated into 3 groups of 30 subjects each. Group I – healthy volunteers without H. pylori infection, group II – subjects with asymptomatic H. pylori infection, group III – H. pylori-infected patients with dyspeptic symptoms, mainly fasting and nocturnal epigastric pain. To diagnose H. pylori infection the urea breath test (UBT-13C) and histological analysis were performed. The level of mRNA expression of tryptophan hydroxylase (TpH-1) was estimated in gastric mucosa with RT-PCR. Results The expression of this enzyme in antral mucosa was 2.69 ±0.97 in group I, 2.28 ±0.69 in group II (p > 0.05) and 4.40 ±1.64 in group III (p 0.05) and 3.40 ±1.51 (p < 0.001), respectively. In group III a positive correlation was found between intensity of H. pylori colonization and TpH-1 expression as well as between TpH-1 expression and severity of dyspeptic symptoms. Conclusions Increased expression of TpH-1 in gastric mucosa plays a role in pathogenesis of chronic dyspepsia.
format	article
author	Cezary Chojnacki Tomasz Popławski Aleksandra Błońska Janusz Błasiak Marek Romanowski Jan Chojnacki
author_facet	Cezary Chojnacki Tomasz Popławski Aleksandra Błońska Janusz Błasiak Marek Romanowski Jan Chojnacki
author_sort	Cezary Chojnacki
title	Expression of tryptophan hydroxylase in gastric mucosa in symptomatic and asymptomatic Helicobacter pylori infection
title_short	Expression of tryptophan hydroxylase in gastric mucosa in symptomatic and asymptomatic Helicobacter pylori infection
title_full	Expression of tryptophan hydroxylase in gastric mucosa in symptomatic and asymptomatic Helicobacter pylori infection
title_fullStr	Expression of tryptophan hydroxylase in gastric mucosa in symptomatic and asymptomatic Helicobacter pylori infection
title_full_unstemmed	Expression of tryptophan hydroxylase in gastric mucosa in symptomatic and asymptomatic Helicobacter pylori infection
title_sort	expression of tryptophan hydroxylase in gastric mucosa in symptomatic and asymptomatic helicobacter pylori infection
publisher	Termedia Publishing House
publishDate	2019
url	https://doaj.org/article/3b86cd94175743168cdea80dbba7adce
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Expression of tryptophan hydroxylase in gastric mucosa in symptomatic and asymptomatic Helicobacter pylori infection

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