Expanding the RpoS/σS-network by RNA sequencing and identification of σS-controlled small RNAs in Salmonella.

The RpoS/σS sigma subunit of RNA polymerase (RNAP) controls a global adaptive response that allows many Gram-negative bacteria to survive starvation and various stresses. σS also contributes to biofilm formation and virulence of the food-borne pathogen Salmonella enterica serovar Typhimurium (S. Typ...

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Autores principales:	Corinne Lévi-Meyrueis, Véronique Monteil, Odile Sismeiro, Marie-Agnès Dillies, Marc Monot, Bernd Jagla, Jean-Yves Coppée, Bruno Dupuy, Françoise Norel
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3b91becb620a4317aef71ed8c90ce4b7
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spelling	oai:doaj.org-article:3b91becb620a4317aef71ed8c90ce4b72021-11-18T08:20:10ZExpanding the RpoS/σS-network by RNA sequencing and identification of σS-controlled small RNAs in Salmonella.1932-620310.1371/journal.pone.0096918https://doaj.org/article/3b91becb620a4317aef71ed8c90ce4b72014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24810289/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The RpoS/σS sigma subunit of RNA polymerase (RNAP) controls a global adaptive response that allows many Gram-negative bacteria to survive starvation and various stresses. σS also contributes to biofilm formation and virulence of the food-borne pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium). In this study, we used directional RNA-sequencing and complementary assays to explore the σS-dependent transcriptome of S. Typhimurium during late stationary phase in rich medium. This study confirms the large regulatory scope of σS and provides insights into the physiological functions of σS in Salmonella. Extensive regulation by σS of genes involved in metabolism and membrane composition, and down-regulation of the respiratory chain functions, were important features of the σS effects on gene transcription that might confer fitness advantages to bacterial cells and/or populations under starving conditions. As an example, we show that arginine catabolism confers a competitive fitness advantage in stationary phase. This study also provides a firm basis for future studies to address molecular mechanisms of indirect regulation of gene expression by σS. Importantly, the σS-controlled downstream network includes small RNAs that might endow σS with post-transcriptional regulatory functions. Of these, four (RyhB-1/RyhB-2, SdsR, SraL) were known to be controlled by σS and deletion of the sdsR locus had a competitive fitness cost in stationary phase. The σS-dependent control of seven additional sRNAs was confirmed in Northern experiments. These findings will inspire future studies to investigate molecular mechanisms and the physiological impact of post-transcriptional regulation by σS.Corinne Lévi-MeyrueisVéronique MonteilOdile SismeiroMarie-Agnès DilliesMarc MonotBernd JaglaJean-Yves CoppéeBruno DupuyFrançoise NorelPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 5, p e96918 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Corinne Lévi-Meyrueis Véronique Monteil Odile Sismeiro Marie-Agnès Dillies Marc Monot Bernd Jagla Jean-Yves Coppée Bruno Dupuy Françoise Norel Expanding the RpoS/σS-network by RNA sequencing and identification of σS-controlled small RNAs in Salmonella.
description	The RpoS/σS sigma subunit of RNA polymerase (RNAP) controls a global adaptive response that allows many Gram-negative bacteria to survive starvation and various stresses. σS also contributes to biofilm formation and virulence of the food-borne pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium). In this study, we used directional RNA-sequencing and complementary assays to explore the σS-dependent transcriptome of S. Typhimurium during late stationary phase in rich medium. This study confirms the large regulatory scope of σS and provides insights into the physiological functions of σS in Salmonella. Extensive regulation by σS of genes involved in metabolism and membrane composition, and down-regulation of the respiratory chain functions, were important features of the σS effects on gene transcription that might confer fitness advantages to bacterial cells and/or populations under starving conditions. As an example, we show that arginine catabolism confers a competitive fitness advantage in stationary phase. This study also provides a firm basis for future studies to address molecular mechanisms of indirect regulation of gene expression by σS. Importantly, the σS-controlled downstream network includes small RNAs that might endow σS with post-transcriptional regulatory functions. Of these, four (RyhB-1/RyhB-2, SdsR, SraL) were known to be controlled by σS and deletion of the sdsR locus had a competitive fitness cost in stationary phase. The σS-dependent control of seven additional sRNAs was confirmed in Northern experiments. These findings will inspire future studies to investigate molecular mechanisms and the physiological impact of post-transcriptional regulation by σS.
format	article
author	Corinne Lévi-Meyrueis Véronique Monteil Odile Sismeiro Marie-Agnès Dillies Marc Monot Bernd Jagla Jean-Yves Coppée Bruno Dupuy Françoise Norel
author_facet	Corinne Lévi-Meyrueis Véronique Monteil Odile Sismeiro Marie-Agnès Dillies Marc Monot Bernd Jagla Jean-Yves Coppée Bruno Dupuy Françoise Norel
author_sort	Corinne Lévi-Meyrueis
title	Expanding the RpoS/σS-network by RNA sequencing and identification of σS-controlled small RNAs in Salmonella.
title_short	Expanding the RpoS/σS-network by RNA sequencing and identification of σS-controlled small RNAs in Salmonella.
title_full	Expanding the RpoS/σS-network by RNA sequencing and identification of σS-controlled small RNAs in Salmonella.
title_fullStr	Expanding the RpoS/σS-network by RNA sequencing and identification of σS-controlled small RNAs in Salmonella.
title_full_unstemmed	Expanding the RpoS/σS-network by RNA sequencing and identification of σS-controlled small RNAs in Salmonella.
title_sort	expanding the rpos/σs-network by rna sequencing and identification of σs-controlled small rnas in salmonella.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/3b91becb620a4317aef71ed8c90ce4b7
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Expanding the RpoS/σS-network by RNA sequencing and identification of σS-controlled small RNAs in Salmonella.

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