Effect of Exogenous Ghrelin on Lipopolysaccharide Induced Change Livers Enzyme Level in Rats

BACKGROUND AND OBJECTIVE: Bacterial lipopolysaccharide (LPS) is a component of the gram-negative bacterial cell wall and is believed to mediate many of the sequela of infection. The liver plays a central role in the inflammatory response to LPS. Elevation of livers enzyme level is one of changes ind...

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Autores principales:	V Sabzifard, S Ebrahimi Vosta Kalai, J Cheraghi, K Sayeh Miri
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2011
Materias:	ghrelin hepatotoxicity lipopolysaccharide liver enzymes Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/3b98eb7923e343959f0bb97b6124447a
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spelling	oai:doaj.org-article:3b98eb7923e343959f0bb97b6124447a2021-11-10T08:56:34ZEffect of Exogenous Ghrelin on Lipopolysaccharide Induced Change Livers Enzyme Level in Rats1561-41072251-7170https://doaj.org/article/3b98eb7923e343959f0bb97b6124447a2011-05-01T00:00:00Zhttp://jbums.org/article-1-3801-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Bacterial lipopolysaccharide (LPS) is a component of the gram-negative bacterial cell wall and is believed to mediate many of the sequela of infection. The liver plays a central role in the inflammatory response to LPS. Elevation of livers enzyme level is one of changes induced in acute phase of inflammation by infection or tissue damage. Ghrelin is a peptide hormone mainly secreted by the mucosa of the stomach and stimulates growth hormone release. Based on anti-inflammatory effects of Ghrelin, in this study we examined the protective effect of Ghrelin on lipopolysaccharide-induced hepatotoxicity in rat.METHODS: In this study forty male Wistar rats (200-250 g) were randomly divided into 4 groups (N=10). Control group received normal saline, second group received LPS (10 mg/kg body weight, i.p), third group received Ghrelin (4 nmol/kg, i.p), and forth group received LPS + Ghrelin (10 mg/kg and 4nmol/kg, respectively) for 8 days. At the end of the 8th day, animals were anaesthetized and blood samples were collected directly from heart. Hepatotoxicity was evaluated by measuring aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and gamma-glutamyl-transpeptidase (GGT). FINDINGS: Injection of LPS induced a significant increase in AST, ALT, ALP and GGT compared with control group (p<0.001). LPS- induced increases in AST, ALT, ALP and GGT were significantly reduced by treatment with Ghrelin (p<0.001). CONCLUSION: In conclusion, our results suggest that Ghrelin has protective effect against LPS- induced hepatotoxicity and this effect may be mediated by anti inflammatory effect of Ghrelin.V SabzifardS Ebrahimi Vosta KalaiJ CheraghiK Sayeh MiriBabol University of Medical Sciencesarticleghrelinhepatotoxicitylipopolysaccharideliver enzymesMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 13, Iss 3, Pp 34-41 (2011)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	ghrelin hepatotoxicity lipopolysaccharide liver enzymes Medicine R Medicine (General) R5-920
spellingShingle	ghrelin hepatotoxicity lipopolysaccharide liver enzymes Medicine R Medicine (General) R5-920 V Sabzifard S Ebrahimi Vosta Kalai J Cheraghi K Sayeh Miri Effect of Exogenous Ghrelin on Lipopolysaccharide Induced Change Livers Enzyme Level in Rats
description	BACKGROUND AND OBJECTIVE: Bacterial lipopolysaccharide (LPS) is a component of the gram-negative bacterial cell wall and is believed to mediate many of the sequela of infection. The liver plays a central role in the inflammatory response to LPS. Elevation of livers enzyme level is one of changes induced in acute phase of inflammation by infection or tissue damage. Ghrelin is a peptide hormone mainly secreted by the mucosa of the stomach and stimulates growth hormone release. Based on anti-inflammatory effects of Ghrelin, in this study we examined the protective effect of Ghrelin on lipopolysaccharide-induced hepatotoxicity in rat.METHODS: In this study forty male Wistar rats (200-250 g) were randomly divided into 4 groups (N=10). Control group received normal saline, second group received LPS (10 mg/kg body weight, i.p), third group received Ghrelin (4 nmol/kg, i.p), and forth group received LPS + Ghrelin (10 mg/kg and 4nmol/kg, respectively) for 8 days. At the end of the 8th day, animals were anaesthetized and blood samples were collected directly from heart. Hepatotoxicity was evaluated by measuring aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and gamma-glutamyl-transpeptidase (GGT). FINDINGS: Injection of LPS induced a significant increase in AST, ALT, ALP and GGT compared with control group (p<0.001). LPS- induced increases in AST, ALT, ALP and GGT were significantly reduced by treatment with Ghrelin (p<0.001). CONCLUSION: In conclusion, our results suggest that Ghrelin has protective effect against LPS- induced hepatotoxicity and this effect may be mediated by anti inflammatory effect of Ghrelin.
format	article
author	V Sabzifard S Ebrahimi Vosta Kalai J Cheraghi K Sayeh Miri
author_facet	V Sabzifard S Ebrahimi Vosta Kalai J Cheraghi K Sayeh Miri
author_sort	V Sabzifard
title	Effect of Exogenous Ghrelin on Lipopolysaccharide Induced Change Livers Enzyme Level in Rats
title_short	Effect of Exogenous Ghrelin on Lipopolysaccharide Induced Change Livers Enzyme Level in Rats
title_full	Effect of Exogenous Ghrelin on Lipopolysaccharide Induced Change Livers Enzyme Level in Rats
title_fullStr	Effect of Exogenous Ghrelin on Lipopolysaccharide Induced Change Livers Enzyme Level in Rats
title_full_unstemmed	Effect of Exogenous Ghrelin on Lipopolysaccharide Induced Change Livers Enzyme Level in Rats
title_sort	effect of exogenous ghrelin on lipopolysaccharide induced change livers enzyme level in rats
publisher	Babol University of Medical Sciences
publishDate	2011
url	https://doaj.org/article/3b98eb7923e343959f0bb97b6124447a
work_keys_str_mv	AT vsabzifard effectofexogenousghrelinonlipopolysaccharideinducedchangeliversenzymelevelinrats AT sebrahimivostakalai effectofexogenousghrelinonlipopolysaccharideinducedchangeliversenzymelevelinrats AT jcheraghi effectofexogenousghrelinonlipopolysaccharideinducedchangeliversenzymelevelinrats AT ksayehmiri effectofexogenousghrelinonlipopolysaccharideinducedchangeliversenzymelevelinrats
_version_	1718440272617013248

Effect of Exogenous Ghrelin on Lipopolysaccharide Induced Change Livers Enzyme Level in Rats

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