A proline rich protein from the gingival seal around teeth exhibits antimicrobial properties against Porphyromonas gingivalis

Abstract The gingival seal around teeth prevents bacteria from destroying the tooth-supporting tissues and disseminating throughout the body. Porphyromonas gingivalis, a major periodontopathogen, degrades components of the specialized extracellular matrix that mediates attachment of the gingiva to t...

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Autores principales:	Aurélien Fouillen, Charline Mary, Katia Julissa Ponce, Pierre Moffatt, Antonio Nanci
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3b9c7539470d449683391adf587afdfd
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spelling	oai:doaj.org-article:3b9c7539470d449683391adf587afdfd2021-12-02T14:16:48ZA proline rich protein from the gingival seal around teeth exhibits antimicrobial properties against Porphyromonas gingivalis10.1038/s41598-021-81791-72045-2322https://doaj.org/article/3b9c7539470d449683391adf587afdfd2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81791-7https://doaj.org/toc/2045-2322Abstract The gingival seal around teeth prevents bacteria from destroying the tooth-supporting tissues and disseminating throughout the body. Porphyromonas gingivalis, a major periodontopathogen, degrades components of the specialized extracellular matrix that mediates attachment of the gingiva to the tooth. Of these, secretory calcium-binding phosphoprotein proline-glutamine rich 1 (SCPPPQ1) protein has a distinctive resistance to degradation, suggesting that it may offer resistance to bacterial attack. In silico analysis of its amino acid sequence was used to explore its molecular characteristics and to predict its two- and three-dimensional structure. SCPPPQ1 exhibits similarities with both proline-rich and cationic antimicrobial proteins, suggesting a putative antimicrobial potential. A combination of imaging approaches showed that incubation with 20 μM of purified SCPPPQ1 decrease bacterial number (p < 0.01). Fluorescence intensity decreased by 70% following a 2 h incubation of Porphyromonas gingivalis with the protein. Electron microscopy analyses revealed that SCPPPQ1 induced bacterial membrane disruption and breaches. While SCPPPQ1 has no effect on mammalian cells, our results suggest that it is bactericidal to Porphyromonas gingivalis, and that this protein, normally present in the gingival seal, may be exploited to maintain a healthy seal and prevent systemic dissemination of bacteria.Aurélien FouillenCharline MaryKatia Julissa PoncePierre MoffattAntonio NanciNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aurélien Fouillen Charline Mary Katia Julissa Ponce Pierre Moffatt Antonio Nanci A proline rich protein from the gingival seal around teeth exhibits antimicrobial properties against Porphyromonas gingivalis
description	Abstract The gingival seal around teeth prevents bacteria from destroying the tooth-supporting tissues and disseminating throughout the body. Porphyromonas gingivalis, a major periodontopathogen, degrades components of the specialized extracellular matrix that mediates attachment of the gingiva to the tooth. Of these, secretory calcium-binding phosphoprotein proline-glutamine rich 1 (SCPPPQ1) protein has a distinctive resistance to degradation, suggesting that it may offer resistance to bacterial attack. In silico analysis of its amino acid sequence was used to explore its molecular characteristics and to predict its two- and three-dimensional structure. SCPPPQ1 exhibits similarities with both proline-rich and cationic antimicrobial proteins, suggesting a putative antimicrobial potential. A combination of imaging approaches showed that incubation with 20 μM of purified SCPPPQ1 decrease bacterial number (p < 0.01). Fluorescence intensity decreased by 70% following a 2 h incubation of Porphyromonas gingivalis with the protein. Electron microscopy analyses revealed that SCPPPQ1 induced bacterial membrane disruption and breaches. While SCPPPQ1 has no effect on mammalian cells, our results suggest that it is bactericidal to Porphyromonas gingivalis, and that this protein, normally present in the gingival seal, may be exploited to maintain a healthy seal and prevent systemic dissemination of bacteria.
format	article
author	Aurélien Fouillen Charline Mary Katia Julissa Ponce Pierre Moffatt Antonio Nanci
author_facet	Aurélien Fouillen Charline Mary Katia Julissa Ponce Pierre Moffatt Antonio Nanci
author_sort	Aurélien Fouillen
title	A proline rich protein from the gingival seal around teeth exhibits antimicrobial properties against Porphyromonas gingivalis
title_short	A proline rich protein from the gingival seal around teeth exhibits antimicrobial properties against Porphyromonas gingivalis
title_full	A proline rich protein from the gingival seal around teeth exhibits antimicrobial properties against Porphyromonas gingivalis
title_fullStr	A proline rich protein from the gingival seal around teeth exhibits antimicrobial properties against Porphyromonas gingivalis
title_full_unstemmed	A proline rich protein from the gingival seal around teeth exhibits antimicrobial properties against Porphyromonas gingivalis
title_sort	proline rich protein from the gingival seal around teeth exhibits antimicrobial properties against porphyromonas gingivalis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3b9c7539470d449683391adf587afdfd
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A proline rich protein from the gingival seal around teeth exhibits antimicrobial properties against Porphyromonas gingivalis

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