Effect of young exosomes injected in aged mice

Bo-Ram Lee,1,* Jung-Hee Kim,1,* Eun-Sook Choi,1,2 Jung-Hoon Cho,3 Eunjoo Kim1,2 1Companion Diagnostics and Medical Technology Research Group, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu 42988, Republic of Korea; 2Global Center for Bio-Convergence Spin System (BicSPINS), Daegu...

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Autores principales:	Lee BR, Kim JH, Choi ES, Cho JH, Kim E
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	exosome injection reverse aging telomerase biomarker molecular therapy Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/3ba061f3c83740bb809cea37e1facf54
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spelling	oai:doaj.org-article:3ba061f3c83740bb809cea37e1facf542021-12-02T00:52:38ZEffect of young exosomes injected in aged mice1178-2013https://doaj.org/article/3ba061f3c83740bb809cea37e1facf542018-09-01T00:00:00Zhttps://www.dovepress.com/effect-of-young-exosomes-injected-in-aged-mice-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Bo-Ram Lee,1,* Jung-Hee Kim,1,* Eun-Sook Choi,1,2 Jung-Hoon Cho,3 Eunjoo Kim1,2 1Companion Diagnostics and Medical Technology Research Group, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu 42988, Republic of Korea; 2Global Center for Bio-Convergence Spin System (BicSPINS), Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu 42988, Republic of Korea; 3School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), Pohang 37666, Republic of Korea *These authors contributed equally to this work Introduction: Exosomes, nano-sized extracellular vesicles, are known to circulate through the blood stream to transfer molecular signals from tissue to tissue. Methods: To determine whether exosomes affect aging in animals, we primarily identified the changes in exosomal miRNA contents during the aging process. In exosomes from 12-month-old mice, mmu-miR-126-5p and mmu-miR-466c-5p levels were decreased and mmu-miR-184-3p and mmu-miR-200b-5p levels were increased significantly compared with those of 3-month-old mice. Their levels in exosomes were partially correlated with those in tissues: levels of only mmu-miR-126-5p and mmu-miR-466c-5p in lungs and/or liver were decreased, but those of mmu-miR-184-3p and mmu-miR-200b-5p in tissues did not coincide with those of exosomes. Results and discussion: In the aged tissues injected with young exosomes isolated from serum, mmu-miR-126b-5p levels were reversed in the lungs and liver. Expression changes in aging-associated molecules in young exosome-injected mice were obvious: p16Ink4A, MTOR, and IGF1R were significantly downregulated in the lungs and/or liver of old mice. In addition, telomerase-related genes such as Men1, Mre11a, Tep1, Terf2, Tert, and Tnks were significantly upregulated in the liver of old mice after injection of young exosomes. Conclusion: These results indicate that exosomes from young mice could reverse the expression pattern of aging-associated molecules in aged mice. Eventually, exosomes may be used as a novel approach for the treatment and diagnosis of aging animals. Keywords: exosome, injection, reverse aging, telomerase, biomarker, molecular therapyLee BRKim JHChoi ESCho JHKim EDove Medical Pressarticleexosomeinjectionreverse agingtelomerasebiomarkermolecular therapyMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 5335-5345 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	exosome injection reverse aging telomerase biomarker molecular therapy Medicine (General) R5-920
spellingShingle	exosome injection reverse aging telomerase biomarker molecular therapy Medicine (General) R5-920 Lee BR Kim JH Choi ES Cho JH Kim E Effect of young exosomes injected in aged mice
description	Bo-Ram Lee,1,* Jung-Hee Kim,1,* Eun-Sook Choi,1,2 Jung-Hoon Cho,3 Eunjoo Kim1,2 1Companion Diagnostics and Medical Technology Research Group, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu 42988, Republic of Korea; 2Global Center for Bio-Convergence Spin System (BicSPINS), Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu 42988, Republic of Korea; 3School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), Pohang 37666, Republic of Korea *These authors contributed equally to this work Introduction: Exosomes, nano-sized extracellular vesicles, are known to circulate through the blood stream to transfer molecular signals from tissue to tissue. Methods: To determine whether exosomes affect aging in animals, we primarily identified the changes in exosomal miRNA contents during the aging process. In exosomes from 12-month-old mice, mmu-miR-126-5p and mmu-miR-466c-5p levels were decreased and mmu-miR-184-3p and mmu-miR-200b-5p levels were increased significantly compared with those of 3-month-old mice. Their levels in exosomes were partially correlated with those in tissues: levels of only mmu-miR-126-5p and mmu-miR-466c-5p in lungs and/or liver were decreased, but those of mmu-miR-184-3p and mmu-miR-200b-5p in tissues did not coincide with those of exosomes. Results and discussion: In the aged tissues injected with young exosomes isolated from serum, mmu-miR-126b-5p levels were reversed in the lungs and liver. Expression changes in aging-associated molecules in young exosome-injected mice were obvious: p16Ink4A, MTOR, and IGF1R were significantly downregulated in the lungs and/or liver of old mice. In addition, telomerase-related genes such as Men1, Mre11a, Tep1, Terf2, Tert, and Tnks were significantly upregulated in the liver of old mice after injection of young exosomes. Conclusion: These results indicate that exosomes from young mice could reverse the expression pattern of aging-associated molecules in aged mice. Eventually, exosomes may be used as a novel approach for the treatment and diagnosis of aging animals. Keywords: exosome, injection, reverse aging, telomerase, biomarker, molecular therapy
format	article
author	Lee BR Kim JH Choi ES Cho JH Kim E
author_facet	Lee BR Kim JH Choi ES Cho JH Kim E
author_sort	Lee BR
title	Effect of young exosomes injected in aged mice
title_short	Effect of young exosomes injected in aged mice
title_full	Effect of young exosomes injected in aged mice
title_fullStr	Effect of young exosomes injected in aged mice
title_full_unstemmed	Effect of young exosomes injected in aged mice
title_sort	effect of young exosomes injected in aged mice
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/3ba061f3c83740bb809cea37e1facf54
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Effect of young exosomes injected in aged mice

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