Automated home-cage for the evaluation of innate non-reflexive pain behaviors in a mouse model of inflammatory pain

Abstract The failure to develop analgesic drugs is attributed not only to the complex and diverse pathophysiology of pain in humans but also to the poor experimental design and poor preclinical assessment of pain. Although considerable efforts have been devoted to overcoming the relevant problems, m...

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Autores principales:	Hasriadi, Peththa Wadu Dasuni Wasana, Opa Vajragupta, Pornchai Rojsitthisak, Pasarapa Towiwat
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3ba4d6e95214489b9025bfddbe6368a2
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spelling	oai:doaj.org-article:3ba4d6e95214489b9025bfddbe6368a22021-12-02T14:58:14ZAutomated home-cage for the evaluation of innate non-reflexive pain behaviors in a mouse model of inflammatory pain10.1038/s41598-021-91444-42045-2322https://doaj.org/article/3ba4d6e95214489b9025bfddbe6368a22021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91444-4https://doaj.org/toc/2045-2322Abstract The failure to develop analgesic drugs is attributed not only to the complex and diverse pathophysiology of pain in humans but also to the poor experimental design and poor preclinical assessment of pain. Although considerable efforts have been devoted to overcoming the relevant problems, many features of the behavioral pain assessment remain to be characterized. For example, a decreased locomotor activity as a common presentation of pain-like behavior has yet to be described. Studies on mice experimentally induced with carrageenan have provided opportunities to explore pain-related behaviors in automated home-cage monitoring. Through this approach, the locomotor activities of mice with carrageenan-induced inflammatory pain can be precisely and objectively captured. Here, we found that the mobile behaviors of mice reduced, and their immobility increased, indicating that carrageenan induction in mice caused a significant decrease in locomotor activity. These non-reflexive pain behaviors were strongly correlated with the reflexive pain behaviors measured via von Frey and plantar tests. Furthermore, the pharmacological intervention using indomethacin improved the locomotor activity of mice with carrageenan-induced pain. Thus, the analysis of the locomotor activity in automated home-cage monitoring is useful for studying the behavioral analgesia and the pharmacological screening of analgesic drugs. The combined evaluation of reflexive and non-reflexive pain behaviors enhances the translational utility of preclinical pain research in rodents.HasriadiPeththa Wadu Dasuni WasanaOpa VajraguptaPornchai RojsitthisakPasarapa TowiwatNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hasriadi Peththa Wadu Dasuni Wasana Opa Vajragupta Pornchai Rojsitthisak Pasarapa Towiwat Automated home-cage for the evaluation of innate non-reflexive pain behaviors in a mouse model of inflammatory pain
description	Abstract The failure to develop analgesic drugs is attributed not only to the complex and diverse pathophysiology of pain in humans but also to the poor experimental design and poor preclinical assessment of pain. Although considerable efforts have been devoted to overcoming the relevant problems, many features of the behavioral pain assessment remain to be characterized. For example, a decreased locomotor activity as a common presentation of pain-like behavior has yet to be described. Studies on mice experimentally induced with carrageenan have provided opportunities to explore pain-related behaviors in automated home-cage monitoring. Through this approach, the locomotor activities of mice with carrageenan-induced inflammatory pain can be precisely and objectively captured. Here, we found that the mobile behaviors of mice reduced, and their immobility increased, indicating that carrageenan induction in mice caused a significant decrease in locomotor activity. These non-reflexive pain behaviors were strongly correlated with the reflexive pain behaviors measured via von Frey and plantar tests. Furthermore, the pharmacological intervention using indomethacin improved the locomotor activity of mice with carrageenan-induced pain. Thus, the analysis of the locomotor activity in automated home-cage monitoring is useful for studying the behavioral analgesia and the pharmacological screening of analgesic drugs. The combined evaluation of reflexive and non-reflexive pain behaviors enhances the translational utility of preclinical pain research in rodents.
format	article
author	Hasriadi Peththa Wadu Dasuni Wasana Opa Vajragupta Pornchai Rojsitthisak Pasarapa Towiwat
author_facet	Hasriadi Peththa Wadu Dasuni Wasana Opa Vajragupta Pornchai Rojsitthisak Pasarapa Towiwat
author_sort	Hasriadi
title	Automated home-cage for the evaluation of innate non-reflexive pain behaviors in a mouse model of inflammatory pain
title_short	Automated home-cage for the evaluation of innate non-reflexive pain behaviors in a mouse model of inflammatory pain
title_full	Automated home-cage for the evaluation of innate non-reflexive pain behaviors in a mouse model of inflammatory pain
title_fullStr	Automated home-cage for the evaluation of innate non-reflexive pain behaviors in a mouse model of inflammatory pain
title_full_unstemmed	Automated home-cage for the evaluation of innate non-reflexive pain behaviors in a mouse model of inflammatory pain
title_sort	automated home-cage for the evaluation of innate non-reflexive pain behaviors in a mouse model of inflammatory pain
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3ba4d6e95214489b9025bfddbe6368a2
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Automated home-cage for the evaluation of innate non-reflexive pain behaviors in a mouse model of inflammatory pain

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