Development to term of cloned cattle derived from donor cells treated with valproic acid.

Cloning of mammals by somatic cell nuclear transfer (SCNT) is still plagued by low efficiency. The epigenetic modifications established during cellular differentiation are a major factor determining this low efficiency as they act as epigenetic barriers restricting reprogramming of somatic nuclei. I...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Juliano Rodrigues Sangalli, Marcos Roberto Chiaratti, Tiago Henrique Camara De Bem, Reno Roldi de Araújo, Fabiana Fernandes Bressan, Rafael Vilar Sampaio, Felipe Perecin, Lawrence Charles Smith, Willian Allan King, Flávio Vieira Meirelles
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3bac1628e3f042aaa04df9eda5fd09f2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3bac1628e3f042aaa04df9eda5fd09f2
record_format	dspace
spelling	oai:doaj.org-article:3bac1628e3f042aaa04df9eda5fd09f22021-11-11T08:21:53ZDevelopment to term of cloned cattle derived from donor cells treated with valproic acid.1932-620310.1371/journal.pone.0101022https://doaj.org/article/3bac1628e3f042aaa04df9eda5fd09f22014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24959750/?tool=EBIhttps://doaj.org/toc/1932-6203Cloning of mammals by somatic cell nuclear transfer (SCNT) is still plagued by low efficiency. The epigenetic modifications established during cellular differentiation are a major factor determining this low efficiency as they act as epigenetic barriers restricting reprogramming of somatic nuclei. In this regard, most factors that promote chromatin decondensation, including histone deacetylase inhibitors (HDACis), have been found to increase nuclear reprogramming efficiency, making their use common to improve SCNT rates. Herein we used valproic acid (VPA) in SCNT to test whether the treatment of nuclear donor cells with this HDACi improves pre- and post-implantation development of cloned cattle. We found that the treatment of fibroblasts with VPA increased histone acetylation without affecting DNA methylation. Moreover, the treatment with VPA resulted in increased expression of IGF2R and PPARGC1A, but not of POU5F1. However, when treated cells were used as nuclear donors no difference of histone acetylation was found after oocyte reconstruction compared to the use of untreated cells. Moreover, shortly after artificial activation the histone acetylation levels were decreased in the embryos produced with VPA-treated cells. With respect to developmental rates, the use of treated cells as donors resulted in no difference during pre- and post-implantation development. In total, five clones developed to term; three produced with untreated cells and two with VPA-treated cells. Among the calves from treated group, one stillborn calf was delivered at day 270 of gestation whereas the other one was delivered at term but died shortly after birth. Among the calves from the control group, one died seven days after birth whereas the other two are still alive and healthy. Altogether, these results show that in spite of the alterations in fibroblasts resulting from the treatment with VPA, their use as donor cells in SCNT did not improve pre- and post-implantation development of cloned cattle.Juliano Rodrigues SangalliMarcos Roberto ChiarattiTiago Henrique Camara De BemReno Roldi de AraújoFabiana Fernandes BressanRafael Vilar SampaioFelipe PerecinLawrence Charles SmithWillian Allan KingFlávio Vieira MeirellesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e101022 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Juliano Rodrigues Sangalli Marcos Roberto Chiaratti Tiago Henrique Camara De Bem Reno Roldi de Araújo Fabiana Fernandes Bressan Rafael Vilar Sampaio Felipe Perecin Lawrence Charles Smith Willian Allan King Flávio Vieira Meirelles Development to term of cloned cattle derived from donor cells treated with valproic acid.
description	Cloning of mammals by somatic cell nuclear transfer (SCNT) is still plagued by low efficiency. The epigenetic modifications established during cellular differentiation are a major factor determining this low efficiency as they act as epigenetic barriers restricting reprogramming of somatic nuclei. In this regard, most factors that promote chromatin decondensation, including histone deacetylase inhibitors (HDACis), have been found to increase nuclear reprogramming efficiency, making their use common to improve SCNT rates. Herein we used valproic acid (VPA) in SCNT to test whether the treatment of nuclear donor cells with this HDACi improves pre- and post-implantation development of cloned cattle. We found that the treatment of fibroblasts with VPA increased histone acetylation without affecting DNA methylation. Moreover, the treatment with VPA resulted in increased expression of IGF2R and PPARGC1A, but not of POU5F1. However, when treated cells were used as nuclear donors no difference of histone acetylation was found after oocyte reconstruction compared to the use of untreated cells. Moreover, shortly after artificial activation the histone acetylation levels were decreased in the embryos produced with VPA-treated cells. With respect to developmental rates, the use of treated cells as donors resulted in no difference during pre- and post-implantation development. In total, five clones developed to term; three produced with untreated cells and two with VPA-treated cells. Among the calves from treated group, one stillborn calf was delivered at day 270 of gestation whereas the other one was delivered at term but died shortly after birth. Among the calves from the control group, one died seven days after birth whereas the other two are still alive and healthy. Altogether, these results show that in spite of the alterations in fibroblasts resulting from the treatment with VPA, their use as donor cells in SCNT did not improve pre- and post-implantation development of cloned cattle.
format	article
author	Juliano Rodrigues Sangalli Marcos Roberto Chiaratti Tiago Henrique Camara De Bem Reno Roldi de Araújo Fabiana Fernandes Bressan Rafael Vilar Sampaio Felipe Perecin Lawrence Charles Smith Willian Allan King Flávio Vieira Meirelles
author_facet	Juliano Rodrigues Sangalli Marcos Roberto Chiaratti Tiago Henrique Camara De Bem Reno Roldi de Araújo Fabiana Fernandes Bressan Rafael Vilar Sampaio Felipe Perecin Lawrence Charles Smith Willian Allan King Flávio Vieira Meirelles
author_sort	Juliano Rodrigues Sangalli
title	Development to term of cloned cattle derived from donor cells treated with valproic acid.
title_short	Development to term of cloned cattle derived from donor cells treated with valproic acid.
title_full	Development to term of cloned cattle derived from donor cells treated with valproic acid.
title_fullStr	Development to term of cloned cattle derived from donor cells treated with valproic acid.
title_full_unstemmed	Development to term of cloned cattle derived from donor cells treated with valproic acid.
title_sort	development to term of cloned cattle derived from donor cells treated with valproic acid.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/3bac1628e3f042aaa04df9eda5fd09f2
work_keys_str_mv	AT julianorodriguessangalli developmenttotermofclonedcattlederivedfromdonorcellstreatedwithvalproicacid AT marcosrobertochiaratti developmenttotermofclonedcattlederivedfromdonorcellstreatedwithvalproicacid AT tiagohenriquecamaradebem developmenttotermofclonedcattlederivedfromdonorcellstreatedwithvalproicacid AT renoroldidearaujo developmenttotermofclonedcattlederivedfromdonorcellstreatedwithvalproicacid AT fabianafernandesbressan developmenttotermofclonedcattlederivedfromdonorcellstreatedwithvalproicacid AT rafaelvilarsampaio developmenttotermofclonedcattlederivedfromdonorcellstreatedwithvalproicacid AT felipeperecin developmenttotermofclonedcattlederivedfromdonorcellstreatedwithvalproicacid AT lawrencecharlessmith developmenttotermofclonedcattlederivedfromdonorcellstreatedwithvalproicacid AT willianallanking developmenttotermofclonedcattlederivedfromdonorcellstreatedwithvalproicacid AT flaviovieirameirelles developmenttotermofclonedcattlederivedfromdonorcellstreatedwithvalproicacid
_version_	1718439302536364032

Development to term of cloned cattle derived from donor cells treated with valproic acid.

Ejemplares similares