Dendritic cells from Crohn's disease patients show aberrant STAT1 and STAT3 signaling.
Abnormalities of dendritic cells (DCs) and STAT proteins have been reported in Crohn's disease (CD). Studies on JAK/STAT signaling in DCs are, however, lacking in CD. We applied a flowcytometric single-cell-based phosphoepitope assay to evaluate STAT1 and STAT3 pathways in DC subsets from CD pa...
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2013
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oai:doaj.org-article:3bafaa11e0354d1e91441107affa76d92021-11-18T09:00:47ZDendritic cells from Crohn's disease patients show aberrant STAT1 and STAT3 signaling.1932-620310.1371/journal.pone.0070738https://doaj.org/article/3bafaa11e0354d1e91441107affa76d92013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23950992/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Abnormalities of dendritic cells (DCs) and STAT proteins have been reported in Crohn's disease (CD). Studies on JAK/STAT signaling in DCs are, however, lacking in CD. We applied a flowcytometric single-cell-based phosphoepitope assay to evaluate STAT1 and STAT3 pathways in DC subsets from CD patients. In addition, circulating DC counts were determined, together with the activation-related immunophenotype. We found that IL-6- and IFN-α-induced STAT3 phosphorylation and IFN-α-induced STAT1 phosphorylation were impaired in plasmacytoid DCs (pDCs) from CD patients (P = 0.005, P = 0.013, and P = 0.006, respectively). In myeloid DCs (mDCs), IFN-α-induced STAT1 and STAT3 phosphorylation were attenuated (P<0.001 and P = 0.048, respectively), but IL-10-induced STAT3 phosphorylation was enhanced (P = 0.026). IFN-γ-induced STAT1 signaling was intact in both DC subtypes. Elevated plasma IL-6 levels were detected in CD (P = 0.004), which strongly correlated with disease activity (ρ = 0.690, P<0.001) but not with IL-6-induced STAT3 phosphorylation. The numbers of pDCs and BDCA3+ mDCs were decreased, and CD40 expression on CD1c+ mDCs was increased in CD. When elucidating the effect of IL-6 signaling on pDC function, we observed that IL-6 treatment of healthy donor pDCs affected the maturation of and modified the T-cell priming by pDCs, favoring Th2 over Th1 type of response and the expression of IL-10 in T cells. Our results implicate DC signaling in human CD. Reduced IL-6 responsiveness in pDCs, together with the attenuated IFN-α-induced signaling in both DC subtypes, may contribute to the immunological dysregulation in CD patients.Janne K NieminenMirja NiemiTaina SipponenHarri M SaloPaula KlemettiMartti FärkkiläJukka VakkilaOuti VaaralaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e70738 (2013) |
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Medicine R Science Q Janne K Nieminen Mirja Niemi Taina Sipponen Harri M Salo Paula Klemetti Martti Färkkilä Jukka Vakkila Outi Vaarala Dendritic cells from Crohn's disease patients show aberrant STAT1 and STAT3 signaling. |
| description |
Abnormalities of dendritic cells (DCs) and STAT proteins have been reported in Crohn's disease (CD). Studies on JAK/STAT signaling in DCs are, however, lacking in CD. We applied a flowcytometric single-cell-based phosphoepitope assay to evaluate STAT1 and STAT3 pathways in DC subsets from CD patients. In addition, circulating DC counts were determined, together with the activation-related immunophenotype. We found that IL-6- and IFN-α-induced STAT3 phosphorylation and IFN-α-induced STAT1 phosphorylation were impaired in plasmacytoid DCs (pDCs) from CD patients (P = 0.005, P = 0.013, and P = 0.006, respectively). In myeloid DCs (mDCs), IFN-α-induced STAT1 and STAT3 phosphorylation were attenuated (P<0.001 and P = 0.048, respectively), but IL-10-induced STAT3 phosphorylation was enhanced (P = 0.026). IFN-γ-induced STAT1 signaling was intact in both DC subtypes. Elevated plasma IL-6 levels were detected in CD (P = 0.004), which strongly correlated with disease activity (ρ = 0.690, P<0.001) but not with IL-6-induced STAT3 phosphorylation. The numbers of pDCs and BDCA3+ mDCs were decreased, and CD40 expression on CD1c+ mDCs was increased in CD. When elucidating the effect of IL-6 signaling on pDC function, we observed that IL-6 treatment of healthy donor pDCs affected the maturation of and modified the T-cell priming by pDCs, favoring Th2 over Th1 type of response and the expression of IL-10 in T cells. Our results implicate DC signaling in human CD. Reduced IL-6 responsiveness in pDCs, together with the attenuated IFN-α-induced signaling in both DC subtypes, may contribute to the immunological dysregulation in CD patients. |
| format |
article |
| author |
Janne K Nieminen Mirja Niemi Taina Sipponen Harri M Salo Paula Klemetti Martti Färkkilä Jukka Vakkila Outi Vaarala |
| author_facet |
Janne K Nieminen Mirja Niemi Taina Sipponen Harri M Salo Paula Klemetti Martti Färkkilä Jukka Vakkila Outi Vaarala |
| author_sort |
Janne K Nieminen |
| title |
Dendritic cells from Crohn's disease patients show aberrant STAT1 and STAT3 signaling. |
| title_short |
Dendritic cells from Crohn's disease patients show aberrant STAT1 and STAT3 signaling. |
| title_full |
Dendritic cells from Crohn's disease patients show aberrant STAT1 and STAT3 signaling. |
| title_fullStr |
Dendritic cells from Crohn's disease patients show aberrant STAT1 and STAT3 signaling. |
| title_full_unstemmed |
Dendritic cells from Crohn's disease patients show aberrant STAT1 and STAT3 signaling. |
| title_sort |
dendritic cells from crohn's disease patients show aberrant stat1 and stat3 signaling. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/3bafaa11e0354d1e91441107affa76d9 |
| work_keys_str_mv |
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