NF-κB induced the donor liver cold preservation related acute lung injury in rat liver transplantation model.

<h4>Background</h4>We have observed at our clinical work that acute lung injury (ALI) often occurs in patients transplanted with donor livers persevered for long time. So, we conducted this study to investigate the influence of cold preservation time (CPT) of donor liver on ALI induced b...

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Autores principales:	An Jiang, Chang Liu, Yulong Song, Feng Liu, Quanyuan Li, Zheng Wu, Liang Yu, Yi Lv
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3bb186f0e3af4f96b0a0ee5b1d8bf527
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spelling	oai:doaj.org-article:3bb186f0e3af4f96b0a0ee5b1d8bf5272021-11-04T06:08:22ZNF-κB induced the donor liver cold preservation related acute lung injury in rat liver transplantation model.1932-620310.1371/journal.pone.0024960https://doaj.org/article/3bb186f0e3af4f96b0a0ee5b1d8bf5272011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21949808/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>We have observed at our clinical work that acute lung injury (ALI) often occurs in patients transplanted with donor livers persevered for long time. So, we conducted this study to investigate the influence of cold preservation time (CPT) of donor liver on ALI induced by liver transplantation (LT), and further study the role of nuclear factor-κB (NF-κB) in the process.<h4>Methods</h4>Wistar rats were used as donors and recipients to establish orthotopic rat liver transplantation models. Donor livers were preserved at 4°C for different lengths of time. The effect of NF-κB inhibitor, ammonium pyrrolidinedithiocarbamate (PDTC), on ALI was detected. All samples were harvested after 3 h reperfusion. The severity of liver injury was evaluated first. The expressions of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in liver tissue and liver outflow serum were measured respectively. The severity indexes of ALI, the activity of NF-κB and inhibitor-κBα (I-κBα) in lung/liver were measured accordingly.<h4>Results</h4>With the prolonged liver CPT, the liver damage associated indexes and ALI-related indexes all increased significantly. TNF-α and IL-1β in liver outflow serum increased accordingly, and the activity of NF-κB in liver/lung increased correspondingly. All these ALI-associated indexes could be partially reversed by the use of PDTC.<h4>Conclusions</h4>Extended CPT aggravates the damage of donor liver and induces the expressions of TNF-α and IL-1β in liver. These inflammatory factors migrate to lung via liver outflow blood and activate NF-κB in lung, inducing ALI finally. NF-κB may play a critical role in LT-related ALI. Patients with or at risk of ALI may benefit from acute anti-inflammatory treatment with PDTC.An JiangChang LiuYulong SongFeng LiuQuanyuan LiZheng WuLiang YuYi LvPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 9, p e24960 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q An Jiang Chang Liu Yulong Song Feng Liu Quanyuan Li Zheng Wu Liang Yu Yi Lv NF-κB induced the donor liver cold preservation related acute lung injury in rat liver transplantation model.
description	<h4>Background</h4>We have observed at our clinical work that acute lung injury (ALI) often occurs in patients transplanted with donor livers persevered for long time. So, we conducted this study to investigate the influence of cold preservation time (CPT) of donor liver on ALI induced by liver transplantation (LT), and further study the role of nuclear factor-κB (NF-κB) in the process.<h4>Methods</h4>Wistar rats were used as donors and recipients to establish orthotopic rat liver transplantation models. Donor livers were preserved at 4°C for different lengths of time. The effect of NF-κB inhibitor, ammonium pyrrolidinedithiocarbamate (PDTC), on ALI was detected. All samples were harvested after 3 h reperfusion. The severity of liver injury was evaluated first. The expressions of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in liver tissue and liver outflow serum were measured respectively. The severity indexes of ALI, the activity of NF-κB and inhibitor-κBα (I-κBα) in lung/liver were measured accordingly.<h4>Results</h4>With the prolonged liver CPT, the liver damage associated indexes and ALI-related indexes all increased significantly. TNF-α and IL-1β in liver outflow serum increased accordingly, and the activity of NF-κB in liver/lung increased correspondingly. All these ALI-associated indexes could be partially reversed by the use of PDTC.<h4>Conclusions</h4>Extended CPT aggravates the damage of donor liver and induces the expressions of TNF-α and IL-1β in liver. These inflammatory factors migrate to lung via liver outflow blood and activate NF-κB in lung, inducing ALI finally. NF-κB may play a critical role in LT-related ALI. Patients with or at risk of ALI may benefit from acute anti-inflammatory treatment with PDTC.
format	article
author	An Jiang Chang Liu Yulong Song Feng Liu Quanyuan Li Zheng Wu Liang Yu Yi Lv
author_facet	An Jiang Chang Liu Yulong Song Feng Liu Quanyuan Li Zheng Wu Liang Yu Yi Lv
author_sort	An Jiang
title	NF-κB induced the donor liver cold preservation related acute lung injury in rat liver transplantation model.
title_short	NF-κB induced the donor liver cold preservation related acute lung injury in rat liver transplantation model.
title_full	NF-κB induced the donor liver cold preservation related acute lung injury in rat liver transplantation model.
title_fullStr	NF-κB induced the donor liver cold preservation related acute lung injury in rat liver transplantation model.
title_full_unstemmed	NF-κB induced the donor liver cold preservation related acute lung injury in rat liver transplantation model.
title_sort	nf-κb induced the donor liver cold preservation related acute lung injury in rat liver transplantation model.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/3bb186f0e3af4f96b0a0ee5b1d8bf527
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NF-κB induced the donor liver cold preservation related acute lung injury in rat liver transplantation model.

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