Metabolomic and Lipidomic Profiling Identifies The Role of the RNA Editing Pathway in Endometrial Carcinogenesis

Abstract Endometrial cancer (EC) remains the most common malignancy of the genital tract among women in developed countries. Although much research has been performed at genomic, transcriptomic and proteomic level, there is still a significant gap in the metabolomic studies of EC. In order to gain i...

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Autores principales:	Tatiana Altadill, Tyrone M. Dowdy, Kirandeep Gill, Armando Reques, Smrithi S. Menon, Cristian P. Moiola, Carlos Lopez-Gil, Eva Coll, Xavier Matias-Guiu, Silvia Cabrera, Angel Garcia, Jaume Reventos, Stephen W. Byers, Antonio Gil-Moreno, Amrita K. Cheema, Eva Colas
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3bb239e64b3648ca92e1d93bdc810fd3
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spelling	oai:doaj.org-article:3bb239e64b3648ca92e1d93bdc810fd32021-12-02T15:06:02ZMetabolomic and Lipidomic Profiling Identifies The Role of the RNA Editing Pathway in Endometrial Carcinogenesis10.1038/s41598-017-09169-22045-2322https://doaj.org/article/3bb239e64b3648ca92e1d93bdc810fd32017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09169-2https://doaj.org/toc/2045-2322Abstract Endometrial cancer (EC) remains the most common malignancy of the genital tract among women in developed countries. Although much research has been performed at genomic, transcriptomic and proteomic level, there is still a significant gap in the metabolomic studies of EC. In order to gain insights into altered metabolic pathways in the onset and progression of EC carcinogenesis, we used high resolution mass spectrometry to characterize the metabolomic and lipidomic profile of 39 human EC and 17 healthy endometrial tissue samples. Several pathways including lipids, Kynurenine pathway, endocannabinoids signaling pathway and the RNA editing pathway were found to be dysregulated in EC. The dysregulation of the RNA editing pathway was further investigated in an independent set of 183 human EC tissues and matched controls, using orthogonal approaches. We found that ADAR2 is overexpressed in EC and that the increase in expression positively correlates with the aggressiveness of the tumor. Furthermore, silencing of ADAR2 in three EC cell lines resulted in a decreased proliferation rate, increased apoptosis, and reduced migration capabilities in vitro. Taken together, our results suggest that ADAR2 functions as an oncogene in endometrial carcinogenesis and could be a potential target for improving EC treatment strategies.Tatiana AltadillTyrone M. DowdyKirandeep GillArmando RequesSmrithi S. MenonCristian P. MoiolaCarlos Lopez-GilEva CollXavier Matias-GuiuSilvia CabreraAngel GarciaJaume ReventosStephen W. ByersAntonio Gil-MorenoAmrita K. CheemaEva ColasNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tatiana Altadill Tyrone M. Dowdy Kirandeep Gill Armando Reques Smrithi S. Menon Cristian P. Moiola Carlos Lopez-Gil Eva Coll Xavier Matias-Guiu Silvia Cabrera Angel Garcia Jaume Reventos Stephen W. Byers Antonio Gil-Moreno Amrita K. Cheema Eva Colas Metabolomic and Lipidomic Profiling Identifies The Role of the RNA Editing Pathway in Endometrial Carcinogenesis
description	Abstract Endometrial cancer (EC) remains the most common malignancy of the genital tract among women in developed countries. Although much research has been performed at genomic, transcriptomic and proteomic level, there is still a significant gap in the metabolomic studies of EC. In order to gain insights into altered metabolic pathways in the onset and progression of EC carcinogenesis, we used high resolution mass spectrometry to characterize the metabolomic and lipidomic profile of 39 human EC and 17 healthy endometrial tissue samples. Several pathways including lipids, Kynurenine pathway, endocannabinoids signaling pathway and the RNA editing pathway were found to be dysregulated in EC. The dysregulation of the RNA editing pathway was further investigated in an independent set of 183 human EC tissues and matched controls, using orthogonal approaches. We found that ADAR2 is overexpressed in EC and that the increase in expression positively correlates with the aggressiveness of the tumor. Furthermore, silencing of ADAR2 in three EC cell lines resulted in a decreased proliferation rate, increased apoptosis, and reduced migration capabilities in vitro. Taken together, our results suggest that ADAR2 functions as an oncogene in endometrial carcinogenesis and could be a potential target for improving EC treatment strategies.
format	article
author	Tatiana Altadill Tyrone M. Dowdy Kirandeep Gill Armando Reques Smrithi S. Menon Cristian P. Moiola Carlos Lopez-Gil Eva Coll Xavier Matias-Guiu Silvia Cabrera Angel Garcia Jaume Reventos Stephen W. Byers Antonio Gil-Moreno Amrita K. Cheema Eva Colas
author_facet	Tatiana Altadill Tyrone M. Dowdy Kirandeep Gill Armando Reques Smrithi S. Menon Cristian P. Moiola Carlos Lopez-Gil Eva Coll Xavier Matias-Guiu Silvia Cabrera Angel Garcia Jaume Reventos Stephen W. Byers Antonio Gil-Moreno Amrita K. Cheema Eva Colas
author_sort	Tatiana Altadill
title	Metabolomic and Lipidomic Profiling Identifies The Role of the RNA Editing Pathway in Endometrial Carcinogenesis
title_short	Metabolomic and Lipidomic Profiling Identifies The Role of the RNA Editing Pathway in Endometrial Carcinogenesis
title_full	Metabolomic and Lipidomic Profiling Identifies The Role of the RNA Editing Pathway in Endometrial Carcinogenesis
title_fullStr	Metabolomic and Lipidomic Profiling Identifies The Role of the RNA Editing Pathway in Endometrial Carcinogenesis
title_full_unstemmed	Metabolomic and Lipidomic Profiling Identifies The Role of the RNA Editing Pathway in Endometrial Carcinogenesis
title_sort	metabolomic and lipidomic profiling identifies the role of the rna editing pathway in endometrial carcinogenesis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3bb239e64b3648ca92e1d93bdc810fd3
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Metabolomic and Lipidomic Profiling Identifies The Role of the RNA Editing Pathway in Endometrial Carcinogenesis

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