Single-molecule trapping and spectroscopy reveals photophysical heterogeneity of phycobilisomes quenched by Orange Carotenoid Protein

Upon photoactivation the Orange Carotenoid Protein (OCP) binds to the phycobilisome and prevents damage by thermally dissipating excess energy. Here authors use an Anti-Brownian ELectrokinetic trap to determine the photophysics of single OCP-quenched phycobilisomes and observe two distinct OCP-quenc...

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Autores principales: Allison H. Squires, Peter D. Dahlberg, Haijun Liu, Nikki Cecil M. Magdaong, Robert E. Blankenship, W. E. Moerner
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
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Acceso en línea:https://doaj.org/article/3bb3c1b07c6e4803980d150d3df8d7b4
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spelling oai:doaj.org-article:3bb3c1b07c6e4803980d150d3df8d7b42021-12-02T14:39:00ZSingle-molecule trapping and spectroscopy reveals photophysical heterogeneity of phycobilisomes quenched by Orange Carotenoid Protein10.1038/s41467-019-09084-22041-1723https://doaj.org/article/3bb3c1b07c6e4803980d150d3df8d7b42019-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-09084-2https://doaj.org/toc/2041-1723Upon photoactivation the Orange Carotenoid Protein (OCP) binds to the phycobilisome and prevents damage by thermally dissipating excess energy. Here authors use an Anti-Brownian ELectrokinetic trap to determine the photophysics of single OCP-quenched phycobilisomes and observe two distinct OCP-quenched states with either one or two OCPs bound.Allison H. SquiresPeter D. DahlbergHaijun LiuNikki Cecil M. MagdaongRobert E. BlankenshipW. E. MoernerNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-12 (2019)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Allison H. Squires
Peter D. Dahlberg
Haijun Liu
Nikki Cecil M. Magdaong
Robert E. Blankenship
W. E. Moerner
Single-molecule trapping and spectroscopy reveals photophysical heterogeneity of phycobilisomes quenched by Orange Carotenoid Protein
description Upon photoactivation the Orange Carotenoid Protein (OCP) binds to the phycobilisome and prevents damage by thermally dissipating excess energy. Here authors use an Anti-Brownian ELectrokinetic trap to determine the photophysics of single OCP-quenched phycobilisomes and observe two distinct OCP-quenched states with either one or two OCPs bound.
format article
author Allison H. Squires
Peter D. Dahlberg
Haijun Liu
Nikki Cecil M. Magdaong
Robert E. Blankenship
W. E. Moerner
author_facet Allison H. Squires
Peter D. Dahlberg
Haijun Liu
Nikki Cecil M. Magdaong
Robert E. Blankenship
W. E. Moerner
author_sort Allison H. Squires
title Single-molecule trapping and spectroscopy reveals photophysical heterogeneity of phycobilisomes quenched by Orange Carotenoid Protein
title_short Single-molecule trapping and spectroscopy reveals photophysical heterogeneity of phycobilisomes quenched by Orange Carotenoid Protein
title_full Single-molecule trapping and spectroscopy reveals photophysical heterogeneity of phycobilisomes quenched by Orange Carotenoid Protein
title_fullStr Single-molecule trapping and spectroscopy reveals photophysical heterogeneity of phycobilisomes quenched by Orange Carotenoid Protein
title_full_unstemmed Single-molecule trapping and spectroscopy reveals photophysical heterogeneity of phycobilisomes quenched by Orange Carotenoid Protein
title_sort single-molecule trapping and spectroscopy reveals photophysical heterogeneity of phycobilisomes quenched by orange carotenoid protein
publisher Nature Portfolio
publishDate 2019
url https://doaj.org/article/3bb3c1b07c6e4803980d150d3df8d7b4
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