The Lin28/Let-7 system in early human embryonic tissue and ectopic pregnancy.
Our objective was to determine the expression of the elements of the Lin28/Let-7 system, and related microRNAs (miRNAs), in early stages of human placentation and ectopic pregnancy, as a means to assess the potential role of this molecular hub in the pathogenesis of ectopic gestation. Seventeen pati...
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2014
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oai:doaj.org-article:3bb44425cee14b3c880f91ae68f8a3042021-11-18T08:34:29ZThe Lin28/Let-7 system in early human embryonic tissue and ectopic pregnancy.1932-620310.1371/journal.pone.0087698https://doaj.org/article/3bb44425cee14b3c880f91ae68f8a3042014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24498170/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Our objective was to determine the expression of the elements of the Lin28/Let-7 system, and related microRNAs (miRNAs), in early stages of human placentation and ectopic pregnancy, as a means to assess the potential role of this molecular hub in the pathogenesis of ectopic gestation. Seventeen patients suffering from tubal ectopic pregnancy (cases) and forty-three women with normal on-going gestation that desired voluntary termination of pregnancy (VTOP; controls) were recruited for the study. Embryonic tissues were subjected to RNA extraction and quantitative PCR analyses for LIN28B, Let-7a, miR-132, miR-145 and mir-323-3p were performed. Our results demonstrate that the expression of LIN28B mRNA was barely detectable in embryonic tissue from early stages of gestation and sharply increased thereafter to plateau between gestational weeks 7-9. In contrast, expression levels of Let-7, mir-132 and mir-145 were high in embryonic tissue from early gestations (≤ 6-weeks) and abruptly declined thereafter, especially for Let-7. Opposite trends were detected for mir-323-3p. Embryonic expression of LIN28B mRNA was higher in early stages (≤ 6-weeks) of ectopic pregnancy than in normal gestation. In contrast, Let-7a expression was significantly lower in early ectopic pregnancies, while miR-132 and miR-145 levels were not altered. Expression of mir-323-3p was also suppressed in ectopic embryonic tissue. We are the first to document reciprocal changes in the expression profiles of the gene encoding the RNA-binding protein, LIN28B, and the related miRNAs, Let-7a, mir-132 and mir-145, in early stages of human placentation. This finding suggests the potential involvement of LIN28B/Let-7 (de)regulated pathways in the pathophysiology of ectopic pregnancy in humans.Teresa LozoyaFrancisco DomínguezAntonio Romero-RuizLiliana SteffaniSebastián MartínezMercedes MonterdeBlanca FerriMaria Jose NúñezAinhoaRomero-EspinósOmar ZamoraMarta GurreaSusana Sangiao-AlvarellosOlivia VegaCarlos SimónAntonio PellicerManuel Tena-SemperePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e87698 (2014) |
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DOAJ |
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DOAJ |
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Medicine R Science Q |
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Medicine R Science Q Teresa Lozoya Francisco Domínguez Antonio Romero-Ruiz Liliana Steffani Sebastián Martínez Mercedes Monterde Blanca Ferri Maria Jose Núñez AinhoaRomero-Espinós Omar Zamora Marta Gurrea Susana Sangiao-Alvarellos Olivia Vega Carlos Simón Antonio Pellicer Manuel Tena-Sempere The Lin28/Let-7 system in early human embryonic tissue and ectopic pregnancy. |
| description |
Our objective was to determine the expression of the elements of the Lin28/Let-7 system, and related microRNAs (miRNAs), in early stages of human placentation and ectopic pregnancy, as a means to assess the potential role of this molecular hub in the pathogenesis of ectopic gestation. Seventeen patients suffering from tubal ectopic pregnancy (cases) and forty-three women with normal on-going gestation that desired voluntary termination of pregnancy (VTOP; controls) were recruited for the study. Embryonic tissues were subjected to RNA extraction and quantitative PCR analyses for LIN28B, Let-7a, miR-132, miR-145 and mir-323-3p were performed. Our results demonstrate that the expression of LIN28B mRNA was barely detectable in embryonic tissue from early stages of gestation and sharply increased thereafter to plateau between gestational weeks 7-9. In contrast, expression levels of Let-7, mir-132 and mir-145 were high in embryonic tissue from early gestations (≤ 6-weeks) and abruptly declined thereafter, especially for Let-7. Opposite trends were detected for mir-323-3p. Embryonic expression of LIN28B mRNA was higher in early stages (≤ 6-weeks) of ectopic pregnancy than in normal gestation. In contrast, Let-7a expression was significantly lower in early ectopic pregnancies, while miR-132 and miR-145 levels were not altered. Expression of mir-323-3p was also suppressed in ectopic embryonic tissue. We are the first to document reciprocal changes in the expression profiles of the gene encoding the RNA-binding protein, LIN28B, and the related miRNAs, Let-7a, mir-132 and mir-145, in early stages of human placentation. This finding suggests the potential involvement of LIN28B/Let-7 (de)regulated pathways in the pathophysiology of ectopic pregnancy in humans. |
| format |
article |
| author |
Teresa Lozoya Francisco Domínguez Antonio Romero-Ruiz Liliana Steffani Sebastián Martínez Mercedes Monterde Blanca Ferri Maria Jose Núñez AinhoaRomero-Espinós Omar Zamora Marta Gurrea Susana Sangiao-Alvarellos Olivia Vega Carlos Simón Antonio Pellicer Manuel Tena-Sempere |
| author_facet |
Teresa Lozoya Francisco Domínguez Antonio Romero-Ruiz Liliana Steffani Sebastián Martínez Mercedes Monterde Blanca Ferri Maria Jose Núñez AinhoaRomero-Espinós Omar Zamora Marta Gurrea Susana Sangiao-Alvarellos Olivia Vega Carlos Simón Antonio Pellicer Manuel Tena-Sempere |
| author_sort |
Teresa Lozoya |
| title |
The Lin28/Let-7 system in early human embryonic tissue and ectopic pregnancy. |
| title_short |
The Lin28/Let-7 system in early human embryonic tissue and ectopic pregnancy. |
| title_full |
The Lin28/Let-7 system in early human embryonic tissue and ectopic pregnancy. |
| title_fullStr |
The Lin28/Let-7 system in early human embryonic tissue and ectopic pregnancy. |
| title_full_unstemmed |
The Lin28/Let-7 system in early human embryonic tissue and ectopic pregnancy. |
| title_sort |
lin28/let-7 system in early human embryonic tissue and ectopic pregnancy. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/3bb44425cee14b3c880f91ae68f8a304 |
| work_keys_str_mv |
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