Prophage-mediated dynamics of 'Candidatus Liberibacter asiaticus' populations, the destructive bacterial pathogens of citrus huanglongbing.

Prophages are highly dynamic components in the bacterial genome and play an important role in intraspecies variations. There are at least two prophages in the chromosomes of Candidatus Liberibacter asiaticus' (Las) Floridian isolates. Las is both unculturable and the most prevalent species of L...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lijuan Zhou, Charles A Powell, Wenbin Li, Mike Irey, Yongping Duan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3bb8e5c54ebc45ac9e36630cd8644d12
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3bb8e5c54ebc45ac9e36630cd8644d12
record_format	dspace
spelling	oai:doaj.org-article:3bb8e5c54ebc45ac9e36630cd8644d122021-11-18T08:42:03ZProphage-mediated dynamics of 'Candidatus Liberibacter asiaticus' populations, the destructive bacterial pathogens of citrus huanglongbing.1932-620310.1371/journal.pone.0082248https://doaj.org/article/3bb8e5c54ebc45ac9e36630cd8644d122013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24349235/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Prophages are highly dynamic components in the bacterial genome and play an important role in intraspecies variations. There are at least two prophages in the chromosomes of Candidatus Liberibacter asiaticus' (Las) Floridian isolates. Las is both unculturable and the most prevalent species of Liberibacter pathogens that cause huanglongbing (HLB), a worldwide destructive disease of citrus. In this study, seven new prophage variants resulting from two hyper-variable regions were identified by screening clone libraries of infected citrus, periwinkle and psyllids. Among them, Types A and B share highly conserved sequences and localize within the two prophages, FP1 and FP2, respectively. Although Types B and C were abundant in all three libraries, Type A was much more abundant in the libraries from the Las-infected psyllids than from the Las-infected plants, and Type D was only identified in libraries from the infected host plants but not from the infected psyllids. Sequence analysis of these variants revealed that the variations may result from recombination and rearrangement events. Conventional PCR results using type-specific molecular markers indicated that A, B, C and D are the four most abundant types in Las-infected citrus and periwinkle. However, only three types, A, B and C are abundant in Las-infected psyllids. Typing results for Las-infected citrus field samples indicated that mixed populations of Las bacteria present in Floridian isolates, but only the Type D population was correlated with the blotchy mottle symptom. Extended cloning and sequencing of the Type D region revealed a third prophage/phage in the Las genome, which may derive from the recombination of FP1 and FP2. Dramatic variations in these prophage regions were also found among the global Las isolates. These results are the first to demonstrate the prophage/phage-mediated dynamics of Las populations in plant and insect hosts, and their correlation with insect transmission and disease development.Lijuan ZhouCharles A PowellWenbin LiMike IreyYongping DuanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e82248 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lijuan Zhou Charles A Powell Wenbin Li Mike Irey Yongping Duan Prophage-mediated dynamics of 'Candidatus Liberibacter asiaticus' populations, the destructive bacterial pathogens of citrus huanglongbing.
description	Prophages are highly dynamic components in the bacterial genome and play an important role in intraspecies variations. There are at least two prophages in the chromosomes of Candidatus Liberibacter asiaticus' (Las) Floridian isolates. Las is both unculturable and the most prevalent species of Liberibacter pathogens that cause huanglongbing (HLB), a worldwide destructive disease of citrus. In this study, seven new prophage variants resulting from two hyper-variable regions were identified by screening clone libraries of infected citrus, periwinkle and psyllids. Among them, Types A and B share highly conserved sequences and localize within the two prophages, FP1 and FP2, respectively. Although Types B and C were abundant in all three libraries, Type A was much more abundant in the libraries from the Las-infected psyllids than from the Las-infected plants, and Type D was only identified in libraries from the infected host plants but not from the infected psyllids. Sequence analysis of these variants revealed that the variations may result from recombination and rearrangement events. Conventional PCR results using type-specific molecular markers indicated that A, B, C and D are the four most abundant types in Las-infected citrus and periwinkle. However, only three types, A, B and C are abundant in Las-infected psyllids. Typing results for Las-infected citrus field samples indicated that mixed populations of Las bacteria present in Floridian isolates, but only the Type D population was correlated with the blotchy mottle symptom. Extended cloning and sequencing of the Type D region revealed a third prophage/phage in the Las genome, which may derive from the recombination of FP1 and FP2. Dramatic variations in these prophage regions were also found among the global Las isolates. These results are the first to demonstrate the prophage/phage-mediated dynamics of Las populations in plant and insect hosts, and their correlation with insect transmission and disease development.
format	article
author	Lijuan Zhou Charles A Powell Wenbin Li Mike Irey Yongping Duan
author_facet	Lijuan Zhou Charles A Powell Wenbin Li Mike Irey Yongping Duan
author_sort	Lijuan Zhou
title	Prophage-mediated dynamics of 'Candidatus Liberibacter asiaticus' populations, the destructive bacterial pathogens of citrus huanglongbing.
title_short	Prophage-mediated dynamics of 'Candidatus Liberibacter asiaticus' populations, the destructive bacterial pathogens of citrus huanglongbing.
title_full	Prophage-mediated dynamics of 'Candidatus Liberibacter asiaticus' populations, the destructive bacterial pathogens of citrus huanglongbing.
title_fullStr	Prophage-mediated dynamics of 'Candidatus Liberibacter asiaticus' populations, the destructive bacterial pathogens of citrus huanglongbing.
title_full_unstemmed	Prophage-mediated dynamics of 'Candidatus Liberibacter asiaticus' populations, the destructive bacterial pathogens of citrus huanglongbing.
title_sort	prophage-mediated dynamics of 'candidatus liberibacter asiaticus' populations, the destructive bacterial pathogens of citrus huanglongbing.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/3bb8e5c54ebc45ac9e36630cd8644d12
work_keys_str_mv	AT lijuanzhou prophagemediateddynamicsofcandidatusliberibacterasiaticuspopulationsthedestructivebacterialpathogensofcitrushuanglongbing AT charlesapowell prophagemediateddynamicsofcandidatusliberibacterasiaticuspopulationsthedestructivebacterialpathogensofcitrushuanglongbing AT wenbinli prophagemediateddynamicsofcandidatusliberibacterasiaticuspopulationsthedestructivebacterialpathogensofcitrushuanglongbing AT mikeirey prophagemediateddynamicsofcandidatusliberibacterasiaticuspopulationsthedestructivebacterialpathogensofcitrushuanglongbing AT yongpingduan prophagemediateddynamicsofcandidatusliberibacterasiaticuspopulationsthedestructivebacterialpathogensofcitrushuanglongbing
_version_	1718421439433932800

Prophage-mediated dynamics of 'Candidatus Liberibacter asiaticus' populations, the destructive bacterial pathogens of citrus huanglongbing.

Ejemplares similares