Metasequoia glyptostroboides potentiates anticancer effect against cervical cancer via intrinsic apoptosis pathway

Abstract This study was undertaken to investigate the anticancer effects of organic extracts derived from the floral cones of Metasequoia glyptostroboides. Dried powder of M. glyptostroboides floral cones was subjected to methanol extraction, and the resulting extract was further partitioned by liqu...

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Autores principales:	Hoomin Lee, Cheolwoo Oh, Suji Kim, Debasish Kumar Dey, Hyung Kyo Kim, Vivek K. Bajpai, Young-Kyu Han, Yun Suk Huh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3bc4cd7278fc4c668c21346a830f9729
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spelling	oai:doaj.org-article:3bc4cd7278fc4c668c21346a830f97292021-12-02T15:23:06ZMetasequoia glyptostroboides potentiates anticancer effect against cervical cancer via intrinsic apoptosis pathway10.1038/s41598-020-79573-82045-2322https://doaj.org/article/3bc4cd7278fc4c668c21346a830f97292021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79573-8https://doaj.org/toc/2045-2322Abstract This study was undertaken to investigate the anticancer effects of organic extracts derived from the floral cones of Metasequoia glyptostroboides. Dried powder of M. glyptostroboides floral cones was subjected to methanol extraction, and the resulting extract was further partitioned by liquid–liquid extraction using the organic solvents n-hexane, dichloromethane (DME), chloroform, and ethyl acetate in addition to deionized water. HeLa cervical and COS-7 cells were used as a cancer cell model and normal cell control, respectively. The anticancer effect was evaluated by using the Cell Counting Kit-8 assay. The viability of COS-7 cells was found to be 12-fold higher than that of the HeLa cells under the administration of 50 µg/ml of the DME extract. Further, the sub-G1 population was determined by FACS analysis. The number of cells at the sub-G1 phase, which indicates apoptotic cells, was increased approximately fourfold upon treatment with the DME and CE extracts compared with that in the negative control. Furthermore, RT-qPCR and western blotting were used to quantitate the relative RNA and protein levels of the cell death pathway components, respectively. Our results suggest that the extracts of M. glyptostroboides floral cones, especially the DME extract, which possesses several anticancer components, as determined by GC–MS analysis, could a potential natural anticancer agent.Hoomin LeeCheolwoo OhSuji KimDebasish Kumar DeyHyung Kyo KimVivek K. BajpaiYoung-Kyu HanYun Suk HuhNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hoomin Lee Cheolwoo Oh Suji Kim Debasish Kumar Dey Hyung Kyo Kim Vivek K. Bajpai Young-Kyu Han Yun Suk Huh Metasequoia glyptostroboides potentiates anticancer effect against cervical cancer via intrinsic apoptosis pathway
description	Abstract This study was undertaken to investigate the anticancer effects of organic extracts derived from the floral cones of Metasequoia glyptostroboides. Dried powder of M. glyptostroboides floral cones was subjected to methanol extraction, and the resulting extract was further partitioned by liquid–liquid extraction using the organic solvents n-hexane, dichloromethane (DME), chloroform, and ethyl acetate in addition to deionized water. HeLa cervical and COS-7 cells were used as a cancer cell model and normal cell control, respectively. The anticancer effect was evaluated by using the Cell Counting Kit-8 assay. The viability of COS-7 cells was found to be 12-fold higher than that of the HeLa cells under the administration of 50 µg/ml of the DME extract. Further, the sub-G1 population was determined by FACS analysis. The number of cells at the sub-G1 phase, which indicates apoptotic cells, was increased approximately fourfold upon treatment with the DME and CE extracts compared with that in the negative control. Furthermore, RT-qPCR and western blotting were used to quantitate the relative RNA and protein levels of the cell death pathway components, respectively. Our results suggest that the extracts of M. glyptostroboides floral cones, especially the DME extract, which possesses several anticancer components, as determined by GC–MS analysis, could a potential natural anticancer agent.
format	article
author	Hoomin Lee Cheolwoo Oh Suji Kim Debasish Kumar Dey Hyung Kyo Kim Vivek K. Bajpai Young-Kyu Han Yun Suk Huh
author_facet	Hoomin Lee Cheolwoo Oh Suji Kim Debasish Kumar Dey Hyung Kyo Kim Vivek K. Bajpai Young-Kyu Han Yun Suk Huh
author_sort	Hoomin Lee
title	Metasequoia glyptostroboides potentiates anticancer effect against cervical cancer via intrinsic apoptosis pathway
title_short	Metasequoia glyptostroboides potentiates anticancer effect against cervical cancer via intrinsic apoptosis pathway
title_full	Metasequoia glyptostroboides potentiates anticancer effect against cervical cancer via intrinsic apoptosis pathway
title_fullStr	Metasequoia glyptostroboides potentiates anticancer effect against cervical cancer via intrinsic apoptosis pathway
title_full_unstemmed	Metasequoia glyptostroboides potentiates anticancer effect against cervical cancer via intrinsic apoptosis pathway
title_sort	metasequoia glyptostroboides potentiates anticancer effect against cervical cancer via intrinsic apoptosis pathway
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3bc4cd7278fc4c668c21346a830f9729
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Metasequoia glyptostroboides potentiates anticancer effect against cervical cancer via intrinsic apoptosis pathway

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