Dose and time effect of CdTe quantum dots on antioxidant capacities of the liver and kidneys in mice

Jilong Wang,1,2,* Hubo Sun,1,2,* Peijun Meng,1,2 Mengmeng Wang,1,2 Mi Tian,3 Yamin Xiong,1,2 Xueying Zhang,1,2 Peili Huang1,2 1School of Public Health, Capital Medical University, 2Beijing Key Laboratory of Environmental Toxicology, 3Medical Experiment and Test Center, Capital Medical University, B...

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Autores principales:	Wang J, Sun H, Meng P, Wang M, Tian M, Xiong Y, Zhang X, Huang P
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	quantum dot antioxidative capacity free-radical elimination hydroxyl radical superoxide radical spin-trapping Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/3bc575557ca1408b8dcab00a713859ea
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spelling	oai:doaj.org-article:3bc575557ca1408b8dcab00a713859ea2021-12-02T00:43:23ZDose and time effect of CdTe quantum dots on antioxidant capacities of the liver and kidneys in mice1178-2013https://doaj.org/article/3bc575557ca1408b8dcab00a713859ea2017-09-01T00:00:00Zhttps://www.dovepress.com/dose-and-time-effect-of-cdte-quantum-dots-on-antioxidant-capacities-of-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Jilong Wang,1,2,* Hubo Sun,1,2,* Peijun Meng,1,2 Mengmeng Wang,1,2 Mi Tian,3 Yamin Xiong,1,2 Xueying Zhang,1,2 Peili Huang1,2 1School of Public Health, Capital Medical University, 2Beijing Key Laboratory of Environmental Toxicology, 3Medical Experiment and Test Center, Capital Medical University, Beijing, People’s Republic of China *These authors contributed equally to this work Abstract: Although quantum dot (QD)-induced toxicity occurs due to free radicals, generation of oxidative stress mediated by reactive oxygen species (ROS) formation is considered an important mechanism. However, free radical mechanisms are essentially difficult to elucidate at the molecular level because most biologically relevant free radicals are highly reactive and short-lived, making them difficult to directly detect, especially in vivo. Antioxidants play an important role in preventing or, in most cases, limiting the damage caused by ROS. Healthy people and animals possess many endogenous antioxidative substances that scavenge free radicals in vivo to maintain the redox balance and genome integrity. The antioxidant capacity of an organism is highly important but seldom studied. In this study, the dose and time effects of CdTe QDs on the antioxidant capacities of the liver and kidneys were investigated in mice using the electron paramagnetic resonance (EPR) spin-trapping technique. We found that the liver and kidneys of healthy mice contain specific antioxidant capacities that scavenge ·OH and ·O2-. Furthermore, oxidative stress markers (superoxide dismutase [SOD], catalase [CAT], glutathione peroxidase [GPx], glutathione [GSH] and malondialdehyde [MDA]) were examined. In dose course studies, the free radical scavenging efficiencies of the liver and kidneys were found to gradually decrease with increasing concentration of CdTe QD exposure. The activities and levels of SOD, CAT, GPx and MDA were observed to increase in treated groups, whereas those of GSH were reduced. The time course studies revealed that the QD-induced antioxidant efficiency reduction was time dependent with GSH decrease and could recover after a period of time. These experimental results offer new information on QD toxicity in vivo. Specifically, CdTe QDs can deplete GSH to reduce the elimination ability of the liver and kidneys for ·OH and ·O2-, thus inducing oxidative damage to tissues. Keywords: quantum dot, antioxidative capacity, free radical elimination, hydroxyl radical, superoxide anion radical, spin-trapping Wang JSun HMeng PWang MTian MXiong YZhang XHuang PDove Medical Pressarticlequantum dotantioxidative capacityfree-radical eliminationhydroxyl radicalsuperoxide radicalspin-trappingMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 6425-6435 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	quantum dot antioxidative capacity free-radical elimination hydroxyl radical superoxide radical spin-trapping Medicine (General) R5-920
spellingShingle	quantum dot antioxidative capacity free-radical elimination hydroxyl radical superoxide radical spin-trapping Medicine (General) R5-920 Wang J Sun H Meng P Wang M Tian M Xiong Y Zhang X Huang P Dose and time effect of CdTe quantum dots on antioxidant capacities of the liver and kidneys in mice
description	Jilong Wang,1,2,* Hubo Sun,1,2,* Peijun Meng,1,2 Mengmeng Wang,1,2 Mi Tian,3 Yamin Xiong,1,2 Xueying Zhang,1,2 Peili Huang1,2 1School of Public Health, Capital Medical University, 2Beijing Key Laboratory of Environmental Toxicology, 3Medical Experiment and Test Center, Capital Medical University, Beijing, People’s Republic of China *These authors contributed equally to this work Abstract: Although quantum dot (QD)-induced toxicity occurs due to free radicals, generation of oxidative stress mediated by reactive oxygen species (ROS) formation is considered an important mechanism. However, free radical mechanisms are essentially difficult to elucidate at the molecular level because most biologically relevant free radicals are highly reactive and short-lived, making them difficult to directly detect, especially in vivo. Antioxidants play an important role in preventing or, in most cases, limiting the damage caused by ROS. Healthy people and animals possess many endogenous antioxidative substances that scavenge free radicals in vivo to maintain the redox balance and genome integrity. The antioxidant capacity of an organism is highly important but seldom studied. In this study, the dose and time effects of CdTe QDs on the antioxidant capacities of the liver and kidneys were investigated in mice using the electron paramagnetic resonance (EPR) spin-trapping technique. We found that the liver and kidneys of healthy mice contain specific antioxidant capacities that scavenge ·OH and ·O2-. Furthermore, oxidative stress markers (superoxide dismutase [SOD], catalase [CAT], glutathione peroxidase [GPx], glutathione [GSH] and malondialdehyde [MDA]) were examined. In dose course studies, the free radical scavenging efficiencies of the liver and kidneys were found to gradually decrease with increasing concentration of CdTe QD exposure. The activities and levels of SOD, CAT, GPx and MDA were observed to increase in treated groups, whereas those of GSH were reduced. The time course studies revealed that the QD-induced antioxidant efficiency reduction was time dependent with GSH decrease and could recover after a period of time. These experimental results offer new information on QD toxicity in vivo. Specifically, CdTe QDs can deplete GSH to reduce the elimination ability of the liver and kidneys for ·OH and ·O2-, thus inducing oxidative damage to tissues. Keywords: quantum dot, antioxidative capacity, free radical elimination, hydroxyl radical, superoxide anion radical, spin-trapping
format	article
author	Wang J Sun H Meng P Wang M Tian M Xiong Y Zhang X Huang P
author_facet	Wang J Sun H Meng P Wang M Tian M Xiong Y Zhang X Huang P
author_sort	Wang J
title	Dose and time effect of CdTe quantum dots on antioxidant capacities of the liver and kidneys in mice
title_short	Dose and time effect of CdTe quantum dots on antioxidant capacities of the liver and kidneys in mice
title_full	Dose and time effect of CdTe quantum dots on antioxidant capacities of the liver and kidneys in mice
title_fullStr	Dose and time effect of CdTe quantum dots on antioxidant capacities of the liver and kidneys in mice
title_full_unstemmed	Dose and time effect of CdTe quantum dots on antioxidant capacities of the liver and kidneys in mice
title_sort	dose and time effect of cdte quantum dots on antioxidant capacities of the liver and kidneys in mice
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/3bc575557ca1408b8dcab00a713859ea
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Dose and time effect of CdTe quantum dots on antioxidant capacities of the liver and kidneys in mice

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