Rapid fabricating technique for multi-layered human hepatic cell sheets by forceful contraction of the fibroblast monolayer.

Cell sheet engineering is attracting attention from investigators in various fields, from basic research scientists to clinicians focused on regenerative medicine. However, hepatocytes have a limited proliferation potential in vitro, and it generally takes a several days to form a sheet morphology a...

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Autores principales:	Yusuke Sakai, Makiko Koike, Hideko Hasegawa, Kosho Yamanouchi, Akihiko Soyama, Mitsuhisa Takatsuki, Tamotsu Kuroki, Kazuo Ohashi, Teruo Okano, Susumu Eguchi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3bd66a6285b44476b99318a25242c886
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spelling	oai:doaj.org-article:3bd66a6285b44476b99318a25242c8862021-11-18T09:02:33ZRapid fabricating technique for multi-layered human hepatic cell sheets by forceful contraction of the fibroblast monolayer.1932-620310.1371/journal.pone.0070970https://doaj.org/article/3bd66a6285b44476b99318a25242c8862013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23923035/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Cell sheet engineering is attracting attention from investigators in various fields, from basic research scientists to clinicians focused on regenerative medicine. However, hepatocytes have a limited proliferation potential in vitro, and it generally takes a several days to form a sheet morphology and multi-layered sheets. We herein report our rapid and efficient technique for generating multi-layered human hepatic cell (HepaRG® cell) sheets using pre-cultured fibroblast monolayers derived from human skin (TIG-118 cells) as a feeder layer on a temperature-responsive culture dish. Multi-layered TIG-118/HepaRG cell sheets with a thick morphology were harvested on day 4 of culturing HepaRG cells by forceful contraction of the TIG-118 cells, and the resulting sheet could be easily handled. In addition, the human albumin and alpha 1-antitrypsin synthesis activities of TIG-118/HepaRG cells were approximately 1.2 and 1.3 times higher than those of HepaRG cells, respectively. Therefore, this technique is considered to be a promising modality for rapidly fabricating multi-layered human hepatocyte sheets from cells with limited proliferation potential, and the engineered cell sheet could be used for cell transplantation with highly specific functions.Yusuke SakaiMakiko KoikeHideko HasegawaKosho YamanouchiAkihiko SoyamaMitsuhisa TakatsukiTamotsu KurokiKazuo OhashiTeruo OkanoSusumu EguchiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e70970 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yusuke Sakai Makiko Koike Hideko Hasegawa Kosho Yamanouchi Akihiko Soyama Mitsuhisa Takatsuki Tamotsu Kuroki Kazuo Ohashi Teruo Okano Susumu Eguchi Rapid fabricating technique for multi-layered human hepatic cell sheets by forceful contraction of the fibroblast monolayer.
description	Cell sheet engineering is attracting attention from investigators in various fields, from basic research scientists to clinicians focused on regenerative medicine. However, hepatocytes have a limited proliferation potential in vitro, and it generally takes a several days to form a sheet morphology and multi-layered sheets. We herein report our rapid and efficient technique for generating multi-layered human hepatic cell (HepaRG® cell) sheets using pre-cultured fibroblast monolayers derived from human skin (TIG-118 cells) as a feeder layer on a temperature-responsive culture dish. Multi-layered TIG-118/HepaRG cell sheets with a thick morphology were harvested on day 4 of culturing HepaRG cells by forceful contraction of the TIG-118 cells, and the resulting sheet could be easily handled. In addition, the human albumin and alpha 1-antitrypsin synthesis activities of TIG-118/HepaRG cells were approximately 1.2 and 1.3 times higher than those of HepaRG cells, respectively. Therefore, this technique is considered to be a promising modality for rapidly fabricating multi-layered human hepatocyte sheets from cells with limited proliferation potential, and the engineered cell sheet could be used for cell transplantation with highly specific functions.
format	article
author	Yusuke Sakai Makiko Koike Hideko Hasegawa Kosho Yamanouchi Akihiko Soyama Mitsuhisa Takatsuki Tamotsu Kuroki Kazuo Ohashi Teruo Okano Susumu Eguchi
author_facet	Yusuke Sakai Makiko Koike Hideko Hasegawa Kosho Yamanouchi Akihiko Soyama Mitsuhisa Takatsuki Tamotsu Kuroki Kazuo Ohashi Teruo Okano Susumu Eguchi
author_sort	Yusuke Sakai
title	Rapid fabricating technique for multi-layered human hepatic cell sheets by forceful contraction of the fibroblast monolayer.
title_short	Rapid fabricating technique for multi-layered human hepatic cell sheets by forceful contraction of the fibroblast monolayer.
title_full	Rapid fabricating technique for multi-layered human hepatic cell sheets by forceful contraction of the fibroblast monolayer.
title_fullStr	Rapid fabricating technique for multi-layered human hepatic cell sheets by forceful contraction of the fibroblast monolayer.
title_full_unstemmed	Rapid fabricating technique for multi-layered human hepatic cell sheets by forceful contraction of the fibroblast monolayer.
title_sort	rapid fabricating technique for multi-layered human hepatic cell sheets by forceful contraction of the fibroblast monolayer.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/3bd66a6285b44476b99318a25242c886
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Rapid fabricating technique for multi-layered human hepatic cell sheets by forceful contraction of the fibroblast monolayer.

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