Phenotypic characters and identification CYPs (Cyclophilin) gene in Cucumis melo L. cv. Gama Melon Parfum

Abstract. Wibowo WA, Maryanto SD, Daryono BS. 2021. Phenotypic characters and identification CYPs (Cyclophilin) gene in Cucumis melo L. cv. Gama Melon Parfum. Biodiversitas 22: 3007-3014. Cucumis melo L. cv. Gama Melon Parfum is a new cultivar with a very strong fragrance as its main character. As a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	WIKO ARIF WIBOWO, SIGIT DWI MARYANTO, BUDI SETIADI DARYONO
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	cyclophilin gene; gama melon parfum; morphology; ppiase; volatile compound. Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3bd88e7f14f34c95a97bf429d5f4eb23
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3bd88e7f14f34c95a97bf429d5f4eb23
record_format	dspace
spelling	oai:doaj.org-article:3bd88e7f14f34c95a97bf429d5f4eb232021-11-22T12:14:19ZPhenotypic characters and identification CYPs (Cyclophilin) gene in Cucumis melo L. cv. Gama Melon Parfum1412-033X2085-472210.13057/biodiv/d220601https://doaj.org/article/3bd88e7f14f34c95a97bf429d5f4eb232021-05-01T00:00:00Zhttps://smujo.id/biodiv/article/view/7422https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Wibowo WA, Maryanto SD, Daryono BS. 2021. Phenotypic characters and identification CYPs (Cyclophilin) gene in Cucumis melo L. cv. Gama Melon Parfum. Biodiversitas 22: 3007-3014. Cucumis melo L. cv. Gama Melon Parfum is a new cultivar with a very strong fragrance as its main character. As a new cultivar with unique characters, it is necessary to characterize phenotype and molecular related to the fragrant aroma. The research aimed to study the phenotypic of the fruit of C. melo cv. Gama Melon Parfum (F3) and to identify the CYPs gene as one of the genes that act in encoding volatile compounds. Analysis of qualitative characters was based on International Plant Genetic Resources Institute (IPGRI) and Plant Variety Protection (PVP), phenotypic characters in melons observed by abiding the Rules for Registration of Varieties from the Indonesian Minister of Agriculture Decree No. 700/Kpts/OT.320/D/12/2011, while analysis of quantitative characters was using the ANOVA methods and software PKBT-STAT-2. The methods of molecular characterization included RNA isolation, cDNA synthesis used Reverse Transcriptase-PCR, amplification of DNA target used PCR, visualization of DNA target used electrophoresis, and DNA sequencing. Analysis of the in silico approach was carried out on the CuGenDB Melon database. Analysis of protein sequences and classification was obtained from InterPro. Phylogeny analysis using MEGA-X Software. The results were 18 qualitative characters and 11 quantitative characters were stable and uniform, whereas the molecular characterization of the genes was predicted Cyclophilin with peptidylprolyl isomerase (PPIase) activity and located in chromosome 1 (17059021-17058899).WIKO ARIF WIBOWOSIGIT DWI MARYANTOBUDI SETIADI DARYONOMBI & UNS Soloarticlecyclophilin gene; gama melon parfum; morphology; ppiase; volatile compound.Biology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 6 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cyclophilin gene; gama melon parfum; morphology; ppiase; volatile compound. Biology (General) QH301-705.5
spellingShingle	cyclophilin gene; gama melon parfum; morphology; ppiase; volatile compound. Biology (General) QH301-705.5 WIKO ARIF WIBOWO SIGIT DWI MARYANTO BUDI SETIADI DARYONO Phenotypic characters and identification CYPs (Cyclophilin) gene in Cucumis melo L. cv. Gama Melon Parfum
description	Abstract. Wibowo WA, Maryanto SD, Daryono BS. 2021. Phenotypic characters and identification CYPs (Cyclophilin) gene in Cucumis melo L. cv. Gama Melon Parfum. Biodiversitas 22: 3007-3014. Cucumis melo L. cv. Gama Melon Parfum is a new cultivar with a very strong fragrance as its main character. As a new cultivar with unique characters, it is necessary to characterize phenotype and molecular related to the fragrant aroma. The research aimed to study the phenotypic of the fruit of C. melo cv. Gama Melon Parfum (F3) and to identify the CYPs gene as one of the genes that act in encoding volatile compounds. Analysis of qualitative characters was based on International Plant Genetic Resources Institute (IPGRI) and Plant Variety Protection (PVP), phenotypic characters in melons observed by abiding the Rules for Registration of Varieties from the Indonesian Minister of Agriculture Decree No. 700/Kpts/OT.320/D/12/2011, while analysis of quantitative characters was using the ANOVA methods and software PKBT-STAT-2. The methods of molecular characterization included RNA isolation, cDNA synthesis used Reverse Transcriptase-PCR, amplification of DNA target used PCR, visualization of DNA target used electrophoresis, and DNA sequencing. Analysis of the in silico approach was carried out on the CuGenDB Melon database. Analysis of protein sequences and classification was obtained from InterPro. Phylogeny analysis using MEGA-X Software. The results were 18 qualitative characters and 11 quantitative characters were stable and uniform, whereas the molecular characterization of the genes was predicted Cyclophilin with peptidylprolyl isomerase (PPIase) activity and located in chromosome 1 (17059021-17058899).
format	article
author	WIKO ARIF WIBOWO SIGIT DWI MARYANTO BUDI SETIADI DARYONO
author_facet	WIKO ARIF WIBOWO SIGIT DWI MARYANTO BUDI SETIADI DARYONO
author_sort	WIKO ARIF WIBOWO
title	Phenotypic characters and identification CYPs (Cyclophilin) gene in Cucumis melo L. cv. Gama Melon Parfum
title_short	Phenotypic characters and identification CYPs (Cyclophilin) gene in Cucumis melo L. cv. Gama Melon Parfum
title_full	Phenotypic characters and identification CYPs (Cyclophilin) gene in Cucumis melo L. cv. Gama Melon Parfum
title_fullStr	Phenotypic characters and identification CYPs (Cyclophilin) gene in Cucumis melo L. cv. Gama Melon Parfum
title_full_unstemmed	Phenotypic characters and identification CYPs (Cyclophilin) gene in Cucumis melo L. cv. Gama Melon Parfum
title_sort	phenotypic characters and identification cyps (cyclophilin) gene in cucumis melo l. cv. gama melon parfum
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/3bd88e7f14f34c95a97bf429d5f4eb23
work_keys_str_mv	AT wikoarifwibowo phenotypiccharactersandidentificationcypscyclophilingeneincucumismelolcvgamamelonparfum AT sigitdwimaryanto phenotypiccharactersandidentificationcypscyclophilingeneincucumismelolcvgamamelonparfum AT budisetiadidaryono phenotypiccharactersandidentificationcypscyclophilingeneincucumismelolcvgamamelonparfum
_version_	1718417669268439040

Phenotypic characters and identification CYPs (Cyclophilin) gene in Cucumis melo L. cv. Gama Melon Parfum

Ejemplares similares