Optimized extraction of polyphenolic antioxidants from the leaves of Himalayan Oak species.

In this study heat-assisted extraction conditions were optimized to enhance extraction yield of antioxidant polyphenols from leaves of Himalayan Quercus species. In initial experiments, a five-factor Plackett-Burman design including 12 experimental runs was tested against the total polyphenolic cont...

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Autores principales:	Aseesh Pandey, Tarun Belwal, Sushma Tamta, Ranbeer S Rawal
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3be1dee1d43e4e008fa5955b7af2ed45
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spelling	oai:doaj.org-article:3be1dee1d43e4e008fa5955b7af2ed452021-12-02T20:04:26ZOptimized extraction of polyphenolic antioxidants from the leaves of Himalayan Oak species.1932-620310.1371/journal.pone.0259350https://doaj.org/article/3be1dee1d43e4e008fa5955b7af2ed452021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0259350https://doaj.org/toc/1932-6203In this study heat-assisted extraction conditions were optimized to enhance extraction yield of antioxidant polyphenols from leaves of Himalayan Quercus species. In initial experiments, a five-factor Plackett-Burman design including 12 experimental runs was tested against the total polyphenolic content (TP). Amongst, XA: extraction temperature, XC: solvent concentration and XE: sample-to-solvent ratio had shown significant influence on yield. These influential factors were further subject to a three-factor-three-level Box-Wilson Central Composite Design; including 20 experimental runs and 3D response surface methodology plots were used to determine optimum conditions [i.e. XA: (80°C), XC:(87%), XE: (1g/40ml)].This optimized condition was further used in other Quercus species of western Himalaya, India. The High-Performance Liquid Chromatography (HPLC) revealed occurrence of 12 polyphenols in six screened Quercus species with the highest concentration of catechin followed by gallic acid. Amongest, Q. franchetii and Q. serrata shared maximum numbers of polyphenolic antioxidants (8 in each). This optimized extraction condition of Quercus species can be utilized for precise quantification of polyphenols and their use in pharmaceutical industries as a potential substitute of synthetic polyphenols.Aseesh PandeyTarun BelwalSushma TamtaRanbeer S RawalPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11, p e0259350 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aseesh Pandey Tarun Belwal Sushma Tamta Ranbeer S Rawal Optimized extraction of polyphenolic antioxidants from the leaves of Himalayan Oak species.
description	In this study heat-assisted extraction conditions were optimized to enhance extraction yield of antioxidant polyphenols from leaves of Himalayan Quercus species. In initial experiments, a five-factor Plackett-Burman design including 12 experimental runs was tested against the total polyphenolic content (TP). Amongst, XA: extraction temperature, XC: solvent concentration and XE: sample-to-solvent ratio had shown significant influence on yield. These influential factors were further subject to a three-factor-three-level Box-Wilson Central Composite Design; including 20 experimental runs and 3D response surface methodology plots were used to determine optimum conditions [i.e. XA: (80°C), XC:(87%), XE: (1g/40ml)].This optimized condition was further used in other Quercus species of western Himalaya, India. The High-Performance Liquid Chromatography (HPLC) revealed occurrence of 12 polyphenols in six screened Quercus species with the highest concentration of catechin followed by gallic acid. Amongest, Q. franchetii and Q. serrata shared maximum numbers of polyphenolic antioxidants (8 in each). This optimized extraction condition of Quercus species can be utilized for precise quantification of polyphenols and their use in pharmaceutical industries as a potential substitute of synthetic polyphenols.
format	article
author	Aseesh Pandey Tarun Belwal Sushma Tamta Ranbeer S Rawal
author_facet	Aseesh Pandey Tarun Belwal Sushma Tamta Ranbeer S Rawal
author_sort	Aseesh Pandey
title	Optimized extraction of polyphenolic antioxidants from the leaves of Himalayan Oak species.
title_short	Optimized extraction of polyphenolic antioxidants from the leaves of Himalayan Oak species.
title_full	Optimized extraction of polyphenolic antioxidants from the leaves of Himalayan Oak species.
title_fullStr	Optimized extraction of polyphenolic antioxidants from the leaves of Himalayan Oak species.
title_full_unstemmed	Optimized extraction of polyphenolic antioxidants from the leaves of Himalayan Oak species.
title_sort	optimized extraction of polyphenolic antioxidants from the leaves of himalayan oak species.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/3be1dee1d43e4e008fa5955b7af2ed45
work_keys_str_mv	AT aseeshpandey optimizedextractionofpolyphenolicantioxidantsfromtheleavesofhimalayanoakspecies AT tarunbelwal optimizedextractionofpolyphenolicantioxidantsfromtheleavesofhimalayanoakspecies AT sushmatamta optimizedextractionofpolyphenolicantioxidantsfromtheleavesofhimalayanoakspecies AT ranbeersrawal optimizedextractionofpolyphenolicantioxidantsfromtheleavesofhimalayanoakspecies
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Optimized extraction of polyphenolic antioxidants from the leaves of Himalayan Oak species.

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