CD38 Defines a Subset of B Cells in Rainbow Trout Kidney With High IgM Secreting Capacities

CD38 is a multifunctional molecule that functions both as a transmembrane signaling receptor and as an ectoenzyme with important roles in cell adhesion, calcium regulation and signal transduction. Within the B cell linage, CD38 is expressed in diverse murine B cell subsets, with highest levels in in...

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Autores principales:	Diana Martín, Pedro Perdiguero, Esther Morel, Irene Soleto, J. German Herranz-Jusdado, Luis A. Ramón, Beatriz Abós, Tiehui Wang, Patricia Díaz-Rosales, Carolina Tafalla
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	teleosts B cells CD38 IgM plasmablasts kidney Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/3be8eee27a4a418ead04ad9741fb9177
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spelling	oai:doaj.org-article:3be8eee27a4a418ead04ad9741fb91772021-12-01T17:57:24ZCD38 Defines a Subset of B Cells in Rainbow Trout Kidney With High IgM Secreting Capacities1664-322410.3389/fimmu.2021.773888https://doaj.org/article/3be8eee27a4a418ead04ad9741fb91772021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.773888/fullhttps://doaj.org/toc/1664-3224CD38 is a multifunctional molecule that functions both as a transmembrane signaling receptor and as an ectoenzyme with important roles in cell adhesion, calcium regulation and signal transduction. Within the B cell linage, CD38 is expressed in diverse murine B cell subsets, with highest levels in innate B cell subpopulations such as marginal zone (MZ) B cells or B1 cells. In humans, however, CD38 is transiently expressed on early lymphocyte precursors, is lost on mature B cells and is consistently expressed on terminally differentiated plasma cells. In the present work, we have identified two homologues of mammalian CD38 in rainbow trout (Oncorhynchus mykiss), designating them as CD38A and CD38B. Although constitutively transcribed throughout different tissues in homeostasis, both CD38A and CD38B mRNA levels were significantly up-regulated in head kidney (HK) in response to a viral infection. In this organ, after the generation of a specific monoclonal antibody (mAb) against CD38A, the presence of CD38A+ populations among IgM+ B cells and IgM- leukocytes was investigated by flow cytometry. Interestingly, the percentage of IgM+CD38A+ B cells increased in response to an in vitro stimulation with inactivated Aeromonas salmonicida. Finally, we demonstrated that HK IgM+CD38A+ B cells had an increased IgM secreting capacity than that of cells lacking CD38A on the cell surface, also showing increased transcription levels of genes associated with B cell differentiation. This study strongly suggests a role for CD38 on the B cell differentiation process in teleosts, and provides us with novel tools to discern between B cell subsets in these species.Diana MartínPedro PerdigueroEsther MorelIrene SoletoJ. German Herranz-JusdadoLuis A. RamónBeatriz AbósTiehui WangPatricia Díaz-RosalesCarolina TafallaFrontiers Media S.A.articleteleostsB cellsCD38IgMplasmablastskidneyImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	teleosts B cells CD38 IgM plasmablasts kidney Immunologic diseases. Allergy RC581-607
spellingShingle	teleosts B cells CD38 IgM plasmablasts kidney Immunologic diseases. Allergy RC581-607 Diana Martín Pedro Perdiguero Esther Morel Irene Soleto J. German Herranz-Jusdado Luis A. Ramón Beatriz Abós Tiehui Wang Patricia Díaz-Rosales Carolina Tafalla CD38 Defines a Subset of B Cells in Rainbow Trout Kidney With High IgM Secreting Capacities
description	CD38 is a multifunctional molecule that functions both as a transmembrane signaling receptor and as an ectoenzyme with important roles in cell adhesion, calcium regulation and signal transduction. Within the B cell linage, CD38 is expressed in diverse murine B cell subsets, with highest levels in innate B cell subpopulations such as marginal zone (MZ) B cells or B1 cells. In humans, however, CD38 is transiently expressed on early lymphocyte precursors, is lost on mature B cells and is consistently expressed on terminally differentiated plasma cells. In the present work, we have identified two homologues of mammalian CD38 in rainbow trout (Oncorhynchus mykiss), designating them as CD38A and CD38B. Although constitutively transcribed throughout different tissues in homeostasis, both CD38A and CD38B mRNA levels were significantly up-regulated in head kidney (HK) in response to a viral infection. In this organ, after the generation of a specific monoclonal antibody (mAb) against CD38A, the presence of CD38A+ populations among IgM+ B cells and IgM- leukocytes was investigated by flow cytometry. Interestingly, the percentage of IgM+CD38A+ B cells increased in response to an in vitro stimulation with inactivated Aeromonas salmonicida. Finally, we demonstrated that HK IgM+CD38A+ B cells had an increased IgM secreting capacity than that of cells lacking CD38A on the cell surface, also showing increased transcription levels of genes associated with B cell differentiation. This study strongly suggests a role for CD38 on the B cell differentiation process in teleosts, and provides us with novel tools to discern between B cell subsets in these species.
format	article
author	Diana Martín Pedro Perdiguero Esther Morel Irene Soleto J. German Herranz-Jusdado Luis A. Ramón Beatriz Abós Tiehui Wang Patricia Díaz-Rosales Carolina Tafalla
author_facet	Diana Martín Pedro Perdiguero Esther Morel Irene Soleto J. German Herranz-Jusdado Luis A. Ramón Beatriz Abós Tiehui Wang Patricia Díaz-Rosales Carolina Tafalla
author_sort	Diana Martín
title	CD38 Defines a Subset of B Cells in Rainbow Trout Kidney With High IgM Secreting Capacities
title_short	CD38 Defines a Subset of B Cells in Rainbow Trout Kidney With High IgM Secreting Capacities
title_full	CD38 Defines a Subset of B Cells in Rainbow Trout Kidney With High IgM Secreting Capacities
title_fullStr	CD38 Defines a Subset of B Cells in Rainbow Trout Kidney With High IgM Secreting Capacities
title_full_unstemmed	CD38 Defines a Subset of B Cells in Rainbow Trout Kidney With High IgM Secreting Capacities
title_sort	cd38 defines a subset of b cells in rainbow trout kidney with high igm secreting capacities
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/3be8eee27a4a418ead04ad9741fb9177
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CD38 Defines a Subset of B Cells in Rainbow Trout Kidney With High IgM Secreting Capacities

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