Transcriptional insights into the pyramided resistance to rice bacterial blight

Abstract The pyramiding of resistance (R) genes provides broad-spectrum and durable resistance to plant diseases. However, the genetic basis for bacterial blight (BB) resistance remains unclear. The BB R gene pyramided line IRBB54, which expresses xa5 and Xa21, possessed a higher level of resistance...

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Autores principales:	Lifen Gao, Zhiwei Fang, Junfei Zhou, Lun Li, Long Lu, Lili Li, Tiantian Li, Lihong Chen, Weixiong Zhang, Wenxue Zhai, Hai Peng
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3bee81b176f04b19a5ce2cd6cae4a7eb
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spelling	oai:doaj.org-article:3bee81b176f04b19a5ce2cd6cae4a7eb2021-12-02T15:08:14ZTranscriptional insights into the pyramided resistance to rice bacterial blight10.1038/s41598-018-29899-12045-2322https://doaj.org/article/3bee81b176f04b19a5ce2cd6cae4a7eb2018-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-29899-1https://doaj.org/toc/2045-2322Abstract The pyramiding of resistance (R) genes provides broad-spectrum and durable resistance to plant diseases. However, the genetic basis for bacterial blight (BB) resistance remains unclear. The BB R gene pyramided line IRBB54, which expresses xa5 and Xa21, possessed a higher level of resistance than both single R gene lines. Large-scale genotyping of genetic markers in this study revealed similar genetic backgrounds among the near-isogenic lines (NILs), suggesting that resistance in the resistant NILs was mainly conferred by the individual R genes or the interaction between them. Transcriptome analysis demonstrated that more than 50% of the differentially expressed genes (DEGs), and more than 70% of the differentially expressed functions, were shared between IRBB54 and IRBB5 or IRBB21. Most of the DEGs in the resistant NILs were downregulated and are predicted to function in cellular and biological process. The DEGs common among the resistant NILs mainly showed non-additive expression patterns and enrichment in stress-related pathways. The differential expression of agronomic trait-controlled genes in the resistant NILs, especially in IRBB54, indicated the existence of potential side-effects resulting from gene pyramiding. Our findings contribute to the understanding of R gene pyramiding, as well as its effects on targeted and non-targeted trait(s).Lifen GaoZhiwei FangJunfei ZhouLun LiLong LuLili LiTiantian LiLihong ChenWeixiong ZhangWenxue ZhaiHai PengNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lifen Gao Zhiwei Fang Junfei Zhou Lun Li Long Lu Lili Li Tiantian Li Lihong Chen Weixiong Zhang Wenxue Zhai Hai Peng Transcriptional insights into the pyramided resistance to rice bacterial blight
description	Abstract The pyramiding of resistance (R) genes provides broad-spectrum and durable resistance to plant diseases. However, the genetic basis for bacterial blight (BB) resistance remains unclear. The BB R gene pyramided line IRBB54, which expresses xa5 and Xa21, possessed a higher level of resistance than both single R gene lines. Large-scale genotyping of genetic markers in this study revealed similar genetic backgrounds among the near-isogenic lines (NILs), suggesting that resistance in the resistant NILs was mainly conferred by the individual R genes or the interaction between them. Transcriptome analysis demonstrated that more than 50% of the differentially expressed genes (DEGs), and more than 70% of the differentially expressed functions, were shared between IRBB54 and IRBB5 or IRBB21. Most of the DEGs in the resistant NILs were downregulated and are predicted to function in cellular and biological process. The DEGs common among the resistant NILs mainly showed non-additive expression patterns and enrichment in stress-related pathways. The differential expression of agronomic trait-controlled genes in the resistant NILs, especially in IRBB54, indicated the existence of potential side-effects resulting from gene pyramiding. Our findings contribute to the understanding of R gene pyramiding, as well as its effects on targeted and non-targeted trait(s).
format	article
author	Lifen Gao Zhiwei Fang Junfei Zhou Lun Li Long Lu Lili Li Tiantian Li Lihong Chen Weixiong Zhang Wenxue Zhai Hai Peng
author_facet	Lifen Gao Zhiwei Fang Junfei Zhou Lun Li Long Lu Lili Li Tiantian Li Lihong Chen Weixiong Zhang Wenxue Zhai Hai Peng
author_sort	Lifen Gao
title	Transcriptional insights into the pyramided resistance to rice bacterial blight
title_short	Transcriptional insights into the pyramided resistance to rice bacterial blight
title_full	Transcriptional insights into the pyramided resistance to rice bacterial blight
title_fullStr	Transcriptional insights into the pyramided resistance to rice bacterial blight
title_full_unstemmed	Transcriptional insights into the pyramided resistance to rice bacterial blight
title_sort	transcriptional insights into the pyramided resistance to rice bacterial blight
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/3bee81b176f04b19a5ce2cd6cae4a7eb
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Transcriptional insights into the pyramided resistance to rice bacterial blight

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