Seasonal Variability of Photosynthetic Microbial Eukaryotes (<3 µm) in the Kara Sea Revealed by 18S rDNA Metabarcoding of Sediment Trap Fluxes

This survey is the first to explore the seasonal cycle of microbial eukaryote diversity (<3 µm) using the NGS method and a 10-month sediment trap (2018–2019). The long-term trap was deployed from September to June in the northwestern part of the Kara Sea. A water sample collected before the sedim...

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Autores principales:	Tatiana A. Belevich, Irina A. Milyutina, Aleksey V. Troitsky
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	microbial eukaryotes photosynthetic picoeukaryotes sediment trap seasonal succession NGS the Kara Sea Botany QK1-989
Acceso en línea:	https://doaj.org/article/3bf6b4d5145f412687aa8cf67027e705
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spelling	oai:doaj.org-article:3bf6b4d5145f412687aa8cf67027e7052021-11-25T18:46:15ZSeasonal Variability of Photosynthetic Microbial Eukaryotes (<3 µm) in the Kara Sea Revealed by 18S rDNA Metabarcoding of Sediment Trap Fluxes10.3390/plants101123942223-7747https://doaj.org/article/3bf6b4d5145f412687aa8cf67027e7052021-11-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2394https://doaj.org/toc/2223-7747This survey is the first to explore the seasonal cycle of microbial eukaryote diversity (<3 µm) using the NGS method and a 10-month sediment trap (2018–2019). The long-term trap was deployed from September to June in the northwestern part of the Kara Sea. A water sample collected before the sediment trap was deployed and also analyzed. The taxonomic composition of microbial eukaryotes in the water sample significantly differed from sediment trap samples, characterized by a high abundance of Ciliophora reads and low abundance of Fungi while trap samples contained an order of magnitude less Ciliophora sequences and high contribution of Fungi. Photosynthetic eukaryotes (PEs) accounting for about 34% of total protists reads were assigned to five major divisions: Chlorophyta, Cryptophyta, Dinoflagellata, Haptophyta, and Ochrophyta. The domination of phototrophic algae was revealed in late autumn. Mamiellophyceae and Trebouxiophyceae were the predominant PEs in mostly all of the studied seasons. <i>Micromonas polaris</i> was constantly present throughout the September–June period in the PE community. The obtained results determine the seasonal dynamics of picoplankton in order to improve our understanding of their role in polar ecosystems.Tatiana A. BelevichIrina A. MilyutinaAleksey V. TroitskyMDPI AGarticlemicrobial eukaryotesphotosynthetic picoeukaryotessediment trapseasonal successionNGSthe Kara SeaBotanyQK1-989ENPlants, Vol 10, Iss 2394, p 2394 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	microbial eukaryotes photosynthetic picoeukaryotes sediment trap seasonal succession NGS the Kara Sea Botany QK1-989
spellingShingle	microbial eukaryotes photosynthetic picoeukaryotes sediment trap seasonal succession NGS the Kara Sea Botany QK1-989 Tatiana A. Belevich Irina A. Milyutina Aleksey V. Troitsky Seasonal Variability of Photosynthetic Microbial Eukaryotes (<3 µm) in the Kara Sea Revealed by 18S rDNA Metabarcoding of Sediment Trap Fluxes
description	This survey is the first to explore the seasonal cycle of microbial eukaryote diversity (<3 µm) using the NGS method and a 10-month sediment trap (2018–2019). The long-term trap was deployed from September to June in the northwestern part of the Kara Sea. A water sample collected before the sediment trap was deployed and also analyzed. The taxonomic composition of microbial eukaryotes in the water sample significantly differed from sediment trap samples, characterized by a high abundance of Ciliophora reads and low abundance of Fungi while trap samples contained an order of magnitude less Ciliophora sequences and high contribution of Fungi. Photosynthetic eukaryotes (PEs) accounting for about 34% of total protists reads were assigned to five major divisions: Chlorophyta, Cryptophyta, Dinoflagellata, Haptophyta, and Ochrophyta. The domination of phototrophic algae was revealed in late autumn. Mamiellophyceae and Trebouxiophyceae were the predominant PEs in mostly all of the studied seasons. <i>Micromonas polaris</i> was constantly present throughout the September–June period in the PE community. The obtained results determine the seasonal dynamics of picoplankton in order to improve our understanding of their role in polar ecosystems.
format	article
author	Tatiana A. Belevich Irina A. Milyutina Aleksey V. Troitsky
author_facet	Tatiana A. Belevich Irina A. Milyutina Aleksey V. Troitsky
author_sort	Tatiana A. Belevich
title	Seasonal Variability of Photosynthetic Microbial Eukaryotes (<3 µm) in the Kara Sea Revealed by 18S rDNA Metabarcoding of Sediment Trap Fluxes
title_short	Seasonal Variability of Photosynthetic Microbial Eukaryotes (<3 µm) in the Kara Sea Revealed by 18S rDNA Metabarcoding of Sediment Trap Fluxes
title_full	Seasonal Variability of Photosynthetic Microbial Eukaryotes (<3 µm) in the Kara Sea Revealed by 18S rDNA Metabarcoding of Sediment Trap Fluxes
title_fullStr	Seasonal Variability of Photosynthetic Microbial Eukaryotes (<3 µm) in the Kara Sea Revealed by 18S rDNA Metabarcoding of Sediment Trap Fluxes
title_full_unstemmed	Seasonal Variability of Photosynthetic Microbial Eukaryotes (<3 µm) in the Kara Sea Revealed by 18S rDNA Metabarcoding of Sediment Trap Fluxes
title_sort	seasonal variability of photosynthetic microbial eukaryotes (<3 µm) in the kara sea revealed by 18s rdna metabarcoding of sediment trap fluxes
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3bf6b4d5145f412687aa8cf67027e705
work_keys_str_mv	AT tatianaabelevich seasonalvariabilityofphotosyntheticmicrobialeukaryotes3μminthekarasearevealedby18srdnametabarcodingofsedimenttrapfluxes AT irinaamilyutina seasonalvariabilityofphotosyntheticmicrobialeukaryotes3μminthekarasearevealedby18srdnametabarcodingofsedimenttrapfluxes AT alekseyvtroitsky seasonalvariabilityofphotosyntheticmicrobialeukaryotes3μminthekarasearevealedby18srdnametabarcodingofsedimenttrapfluxes
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Seasonal Variability of Photosynthetic Microbial Eukaryotes (<3 µm) in the Kara Sea Revealed by 18S rDNA Metabarcoding of Sediment Trap Fluxes

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