IMMUNOHISTOCHEMICAL EXPRESSION OF TRANSDUCER LIKE ENHANCER OF SPLIT 1 (TLE-1) IN CASES OF SYNOVIAL SARCOMA

Objective: To determine the immunohistochemical (IHC) expression of transducer like enhancer of split 1 (TLE-1) in diagnosed cases of synovial sarcoma (SS). Study Design: Cross sectional study. Place and Duration of Study: Department of Histopathology, Armed Forces Institute of Pathology (AFIP...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hamza Mansur, Muhammad Asif, Muhammad Tahir Khadim, Rabia Ahmed, Madiha Anwer, Anza Azhar
Formato:	article
Lenguaje:	EN
Publicado:	Army Medical College Rawalpindi 2019
Materias:	immunohistochemistry spindle cell sarcomas synovial sarcoma Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/3bff45c10771426c802279eefe68385d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3bff45c10771426c802279eefe68385d
record_format	dspace
spelling	oai:doaj.org-article:3bff45c10771426c802279eefe68385d2021-11-10T03:25:17ZIMMUNOHISTOCHEMICAL EXPRESSION OF TRANSDUCER LIKE ENHANCER OF SPLIT 1 (TLE-1) IN CASES OF SYNOVIAL SARCOMA0030-96482411-8842https://doaj.org/article/3bff45c10771426c802279eefe68385d2019-10-01T00:00:00Zhttps://www.pafmj.org/index.php/PAFMJ/article/view/3421/2396https://doaj.org/toc/0030-9648https://doaj.org/toc/2411-8842Objective: To determine the immunohistochemical (IHC) expression of transducer like enhancer of split 1 (TLE-1) in diagnosed cases of synovial sarcoma (SS). Study Design: Cross sectional study. Place and Duration of Study: Department of Histopathology, Armed Forces Institute of Pathology (AFIP), Rawalpindi, Pakistan, from Jan 2017 to Jun 2018. Methodology: In our study, 60 cases of SS diagnosed on hematoxylin & eosin (H&E) morphology, IHC expression of CD99 and epithelial membrane antigen (EMA) were retrieved from archive of Histopathology department AFIP Rawalpindi. Patient’s age, gender, tumor site and histologic type were noted. IHC for TLE-1 was applied and results were recorded. Data was analyzed using SPSS version 22. Results: A total of 60 cases were included in which 33 were males and 27 were females. The male to female ratio was 1.2:1. Mean age of the study patients was 36.6 ± 15 years. SS was found be more common in soft tissues of limbs/ extremities around tendons sheaths (76%); other sites being lungs, head & neck and retroperitoneum. Out of 60 cases of SS, 42 (70%) cases were monophasic, while 15 (25%) and 3 (5%) cases were biphasic and poorly differentiated SS, respectively. A total of 90% samples (n=54) showed immunoreactivity for TLE-1 (p=0.023) out of which 61% samples (n=33) had diffuse (2+) nuclear staining while 21 (39%) showed weak to moderate nuclear staining for TLE-1. However, 6 (10%) cases did not show nuclear staining for TLE-1. Conclusion: TLE-1 was found to be a novel marker which was extremely helpful in diagnosis of SS, especially in cases where diagnostic challenge arises when other spindle cell neoplasms are considered in the differential diagnosis and in laboratory set ups where advanced facilities of molecular testing by fluorescent in situ hybridization (FISH) is not available.Hamza MansurMuhammad AsifMuhammad Tahir KhadimRabia AhmedMadiha AnwerAnza AzharArmy Medical College Rawalpindiarticleimmunohistochemistryspindle cell sarcomassynovial sarcomaMedicineRMedicine (General)R5-920ENPakistan Armed Forces Medical Journal, Vol 69, Iss 5, Pp 1098-1102 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	immunohistochemistry spindle cell sarcomas synovial sarcoma Medicine R Medicine (General) R5-920
spellingShingle	immunohistochemistry spindle cell sarcomas synovial sarcoma Medicine R Medicine (General) R5-920 Hamza Mansur Muhammad Asif Muhammad Tahir Khadim Rabia Ahmed Madiha Anwer Anza Azhar IMMUNOHISTOCHEMICAL EXPRESSION OF TRANSDUCER LIKE ENHANCER OF SPLIT 1 (TLE-1) IN CASES OF SYNOVIAL SARCOMA
description	Objective: To determine the immunohistochemical (IHC) expression of transducer like enhancer of split 1 (TLE-1) in diagnosed cases of synovial sarcoma (SS). Study Design: Cross sectional study. Place and Duration of Study: Department of Histopathology, Armed Forces Institute of Pathology (AFIP), Rawalpindi, Pakistan, from Jan 2017 to Jun 2018. Methodology: In our study, 60 cases of SS diagnosed on hematoxylin & eosin (H&E) morphology, IHC expression of CD99 and epithelial membrane antigen (EMA) were retrieved from archive of Histopathology department AFIP Rawalpindi. Patient’s age, gender, tumor site and histologic type were noted. IHC for TLE-1 was applied and results were recorded. Data was analyzed using SPSS version 22. Results: A total of 60 cases were included in which 33 were males and 27 were females. The male to female ratio was 1.2:1. Mean age of the study patients was 36.6 ± 15 years. SS was found be more common in soft tissues of limbs/ extremities around tendons sheaths (76%); other sites being lungs, head & neck and retroperitoneum. Out of 60 cases of SS, 42 (70%) cases were monophasic, while 15 (25%) and 3 (5%) cases were biphasic and poorly differentiated SS, respectively. A total of 90% samples (n=54) showed immunoreactivity for TLE-1 (p=0.023) out of which 61% samples (n=33) had diffuse (2+) nuclear staining while 21 (39%) showed weak to moderate nuclear staining for TLE-1. However, 6 (10%) cases did not show nuclear staining for TLE-1. Conclusion: TLE-1 was found to be a novel marker which was extremely helpful in diagnosis of SS, especially in cases where diagnostic challenge arises when other spindle cell neoplasms are considered in the differential diagnosis and in laboratory set ups where advanced facilities of molecular testing by fluorescent in situ hybridization (FISH) is not available.
format	article
author	Hamza Mansur Muhammad Asif Muhammad Tahir Khadim Rabia Ahmed Madiha Anwer Anza Azhar
author_facet	Hamza Mansur Muhammad Asif Muhammad Tahir Khadim Rabia Ahmed Madiha Anwer Anza Azhar
author_sort	Hamza Mansur
title	IMMUNOHISTOCHEMICAL EXPRESSION OF TRANSDUCER LIKE ENHANCER OF SPLIT 1 (TLE-1) IN CASES OF SYNOVIAL SARCOMA
title_short	IMMUNOHISTOCHEMICAL EXPRESSION OF TRANSDUCER LIKE ENHANCER OF SPLIT 1 (TLE-1) IN CASES OF SYNOVIAL SARCOMA
title_full	IMMUNOHISTOCHEMICAL EXPRESSION OF TRANSDUCER LIKE ENHANCER OF SPLIT 1 (TLE-1) IN CASES OF SYNOVIAL SARCOMA
title_fullStr	IMMUNOHISTOCHEMICAL EXPRESSION OF TRANSDUCER LIKE ENHANCER OF SPLIT 1 (TLE-1) IN CASES OF SYNOVIAL SARCOMA
title_full_unstemmed	IMMUNOHISTOCHEMICAL EXPRESSION OF TRANSDUCER LIKE ENHANCER OF SPLIT 1 (TLE-1) IN CASES OF SYNOVIAL SARCOMA
title_sort	immunohistochemical expression of transducer like enhancer of split 1 (tle-1) in cases of synovial sarcoma
publisher	Army Medical College Rawalpindi
publishDate	2019
url	https://doaj.org/article/3bff45c10771426c802279eefe68385d
work_keys_str_mv	AT hamzamansur immunohistochemicalexpressionoftransducerlikeenhancerofsplit1tle1incasesofsynovialsarcoma AT muhammadasif immunohistochemicalexpressionoftransducerlikeenhancerofsplit1tle1incasesofsynovialsarcoma AT muhammadtahirkhadim immunohistochemicalexpressionoftransducerlikeenhancerofsplit1tle1incasesofsynovialsarcoma AT rabiaahmed immunohistochemicalexpressionoftransducerlikeenhancerofsplit1tle1incasesofsynovialsarcoma AT madihaanwer immunohistochemicalexpressionoftransducerlikeenhancerofsplit1tle1incasesofsynovialsarcoma AT anzaazhar immunohistochemicalexpressionoftransducerlikeenhancerofsplit1tle1incasesofsynovialsarcoma
_version_	1718440737597554688

IMMUNOHISTOCHEMICAL EXPRESSION OF TRANSDUCER LIKE ENHANCER OF SPLIT 1 (TLE-1) IN CASES OF SYNOVIAL SARCOMA

Ejemplares similares