Quantifying protein densities on cell membranes using super-resolution optical fluctuation imaging

The ability to quantify the organization of cell membrane molecules is limited by the density of labeling and experimental conditions. Here, the authors use super-resolution optical fluctuation (SOFI) for molecular density and clustering analyses, and investigate nanoscale distribution of CD4 glycop...

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Autores principales: Tomáš Lukeš, Daniela Glatzová, Zuzana Kvíčalová, Florian Levet, Aleš Benda, Sebastian Letschert, Markus Sauer, Tomáš Brdička, Theo Lasser, Marek Cebecauer
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Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/3c01c52f65f749888df9da458d18ffa1
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spelling oai:doaj.org-article:3c01c52f65f749888df9da458d18ffa12021-12-02T15:38:54ZQuantifying protein densities on cell membranes using super-resolution optical fluctuation imaging10.1038/s41467-017-01857-x2041-1723https://doaj.org/article/3c01c52f65f749888df9da458d18ffa12017-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-01857-xhttps://doaj.org/toc/2041-1723The ability to quantify the organization of cell membrane molecules is limited by the density of labeling and experimental conditions. Here, the authors use super-resolution optical fluctuation (SOFI) for molecular density and clustering analyses, and investigate nanoscale distribution of CD4 glycoprotein.Tomáš LukešDaniela GlatzováZuzana KvíčalováFlorian LevetAleš BendaSebastian LetschertMarkus SauerTomáš BrdičkaTheo LasserMarek CebecauerNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-7 (2017)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Tomáš Lukeš
Daniela Glatzová
Zuzana Kvíčalová
Florian Levet
Aleš Benda
Sebastian Letschert
Markus Sauer
Tomáš Brdička
Theo Lasser
Marek Cebecauer
Quantifying protein densities on cell membranes using super-resolution optical fluctuation imaging
description The ability to quantify the organization of cell membrane molecules is limited by the density of labeling and experimental conditions. Here, the authors use super-resolution optical fluctuation (SOFI) for molecular density and clustering analyses, and investigate nanoscale distribution of CD4 glycoprotein.
format article
author Tomáš Lukeš
Daniela Glatzová
Zuzana Kvíčalová
Florian Levet
Aleš Benda
Sebastian Letschert
Markus Sauer
Tomáš Brdička
Theo Lasser
Marek Cebecauer
author_facet Tomáš Lukeš
Daniela Glatzová
Zuzana Kvíčalová
Florian Levet
Aleš Benda
Sebastian Letschert
Markus Sauer
Tomáš Brdička
Theo Lasser
Marek Cebecauer
author_sort Tomáš Lukeš
title Quantifying protein densities on cell membranes using super-resolution optical fluctuation imaging
title_short Quantifying protein densities on cell membranes using super-resolution optical fluctuation imaging
title_full Quantifying protein densities on cell membranes using super-resolution optical fluctuation imaging
title_fullStr Quantifying protein densities on cell membranes using super-resolution optical fluctuation imaging
title_full_unstemmed Quantifying protein densities on cell membranes using super-resolution optical fluctuation imaging
title_sort quantifying protein densities on cell membranes using super-resolution optical fluctuation imaging
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/3c01c52f65f749888df9da458d18ffa1
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