Cartilage Tissue Engineering by the 3D Bioprinting of iPS Cells in a Nanocellulose/Alginate Bioink
Cartilage lesions can progress into secondary osteoarthritis and cause severe clinical problems in numerous patients. As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulos...
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Nature Portfolio
2017
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oai:doaj.org-article:3c064b477afd4f1db31a5385f9ae7dfe2021-12-02T11:40:31ZCartilage Tissue Engineering by the 3D Bioprinting of iPS Cells in a Nanocellulose/Alginate Bioink10.1038/s41598-017-00690-y2045-2322https://doaj.org/article/3c064b477afd4f1db31a5385f9ae7dfe2017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00690-yhttps://doaj.org/toc/2045-2322Cartilage lesions can progress into secondary osteoarthritis and cause severe clinical problems in numerous patients. As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes. Two bioinks were investigated: NFC with alginate (NFC/A) or hyaluronic acid (NFC/HA). Low proliferation and phenotypic changes away from pluripotency were seen in the case of NFC/HA. However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs. Moreover, a marked increase in cell number within the cartilaginous tissue was detected by 2-photon fluorescence microscopy, indicating the importance of high cell densities in the pursuit of achieving good survival after printing. We conclude that NFC/A bioink is suitable for bioprinting iPSCs to support cartilage production in co-cultures with irradiated chondrocytes.Duong NguyenDaniel A. HäggAlma ForsmanJosefine EkholmPuwapong NimkingratanaCamilla BrantsingTheodoros KalogeropoulosSamantha ZaunzSebastian ConcaroMats BrittbergAnders LindahlPaul GatenholmAnnika EnejderStina SimonssonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017) |
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Medicine R Science Q Duong Nguyen Daniel A. Hägg Alma Forsman Josefine Ekholm Puwapong Nimkingratana Camilla Brantsing Theodoros Kalogeropoulos Samantha Zaunz Sebastian Concaro Mats Brittberg Anders Lindahl Paul Gatenholm Annika Enejder Stina Simonsson Cartilage Tissue Engineering by the 3D Bioprinting of iPS Cells in a Nanocellulose/Alginate Bioink |
| description |
Cartilage lesions can progress into secondary osteoarthritis and cause severe clinical problems in numerous patients. As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes. Two bioinks were investigated: NFC with alginate (NFC/A) or hyaluronic acid (NFC/HA). Low proliferation and phenotypic changes away from pluripotency were seen in the case of NFC/HA. However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs. Moreover, a marked increase in cell number within the cartilaginous tissue was detected by 2-photon fluorescence microscopy, indicating the importance of high cell densities in the pursuit of achieving good survival after printing. We conclude that NFC/A bioink is suitable for bioprinting iPSCs to support cartilage production in co-cultures with irradiated chondrocytes. |
| format |
article |
| author |
Duong Nguyen Daniel A. Hägg Alma Forsman Josefine Ekholm Puwapong Nimkingratana Camilla Brantsing Theodoros Kalogeropoulos Samantha Zaunz Sebastian Concaro Mats Brittberg Anders Lindahl Paul Gatenholm Annika Enejder Stina Simonsson |
| author_facet |
Duong Nguyen Daniel A. Hägg Alma Forsman Josefine Ekholm Puwapong Nimkingratana Camilla Brantsing Theodoros Kalogeropoulos Samantha Zaunz Sebastian Concaro Mats Brittberg Anders Lindahl Paul Gatenholm Annika Enejder Stina Simonsson |
| author_sort |
Duong Nguyen |
| title |
Cartilage Tissue Engineering by the 3D Bioprinting of iPS Cells in a Nanocellulose/Alginate Bioink |
| title_short |
Cartilage Tissue Engineering by the 3D Bioprinting of iPS Cells in a Nanocellulose/Alginate Bioink |
| title_full |
Cartilage Tissue Engineering by the 3D Bioprinting of iPS Cells in a Nanocellulose/Alginate Bioink |
| title_fullStr |
Cartilage Tissue Engineering by the 3D Bioprinting of iPS Cells in a Nanocellulose/Alginate Bioink |
| title_full_unstemmed |
Cartilage Tissue Engineering by the 3D Bioprinting of iPS Cells in a Nanocellulose/Alginate Bioink |
| title_sort |
cartilage tissue engineering by the 3d bioprinting of ips cells in a nanocellulose/alginate bioink |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/3c064b477afd4f1db31a5385f9ae7dfe |
| work_keys_str_mv |
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