The migration of synthetic magnetic nanoparticle labeled dendritic cells into lymph nodes with optical imaging

Hang Su,1,* Yongbin Mou,1,* Yanli An,2 Wei Han,1 Xiaofeng Huang,1 Guohua Xia,3 Yanhong Ni,1 Yu Zhang,4 Jianmin Ma,1 Qingang Hu1,5 1Center Laboratory of Stomatology, Stomatological Hospital Affiliated Medical School, Nanjing University, Nanjing, People's Republic of China; 2Jiangsu Key Lab of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Su H, Mou YB, An YL, Han W, Huang XF, Xia GH, Ni YH, Zhang Y, Ma JM, Hu QG
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/3c0cae2aa9ee49b481dcaed4bb0e503a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3c0cae2aa9ee49b481dcaed4bb0e503a
record_format	dspace
spelling	oai:doaj.org-article:3c0cae2aa9ee49b481dcaed4bb0e503a2021-12-02T07:14:23ZThe migration of synthetic magnetic nanoparticle labeled dendritic cells into lymph nodes with optical imaging1176-91141178-2013https://doaj.org/article/3c0cae2aa9ee49b481dcaed4bb0e503a2013-10-01T00:00:00Zhttp://www.dovepress.com/the-migration-of-synthetic-magnetic-nanoparticle-labeled-dendritic-cel-a14597https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Hang Su,1,* Yongbin Mou,1,* Yanli An,2 Wei Han,1 Xiaofeng Huang,1 Guohua Xia,3 Yanhong Ni,1 Yu Zhang,4 Jianmin Ma,1 Qingang Hu1,5 1Center Laboratory of Stomatology, Stomatological Hospital Affiliated Medical School, Nanjing University, Nanjing, People's Republic of China; 2Jiangsu Key Lab of Molecular and Function Imaging, Department of Radiology; 3Department of Hematology, Zhongda Hospital, Medical School, 4State Key Laboratory of Molecule and Bimolecular Electronics, Jiangsu Provincial Laboratory for Biomaterials and Devices; Southeast University, Nanjing, People's Republic of China; 5Leeds Dental Institute, Faculty of Medicine and health, University of Leeds, Leeds, United Kingdom*These authors contributed equally to this workBackground: The successful biotherapy of carcinoma with dendritic cell (DC) vaccines pivotally relies on DCs’ migratory capability into lymph tissues and activation of T cells. Accurate imaging and evaluation of DC migration in vivo have great significance during antitumor treatment with DC vaccine. We herein examined the behavior of DCs influenced by synthetic superparamagnetic iron oxide (SPIO) nanoparticle labeling.Methods: γ-Fe2O3 nanoparticles were prepared and DCs, which were induced from bone marrow monocytes of enhanced green fluorescent protein (EGFP) transgenic mice, were labeled. The endocytosis of the SPIO, surface molecules, cell apoptosis and fluorescence intensity of EGFP-DCs were displayed by Prussian blue staining and flow cytometry (FCM), respectively. After EGFP-DCs, labeled with SPIO, were injected into footpads (n = 5) for 24 hours, the mice were examined in vivo by optical imaging (OPI). Meanwhile, confocal imaging and FCM were applied, respectively, to detect the migration of labeled DCs into draining lymph nodes.Results: Nearly 100% of cells were labeled by the SPIO, in which the intracellular blue color gradually deepened and the iron contents rose with the increase of labeling iron concentrations. In addition, cell apoptosis and the surface molecules on DCs were at similar levels after SPIO labeling. After confirming that the fluorescence intensity of EGFP on DCs was not influenced by SPIO, the homing ability of EGFP-DCs labeled with SPIO displayed that the fluorescence intensity and the ratios of EGFP-DCs in draining lymph nodes were gradually decreased with the increase of labeling iron concentrations.Conclusion: The synthetic SPIO nanoparticles possess perfect labeling ability and biocompatibility. Moreover, DCs labeled with a low dose of SPIO showed stronger migratory capability in vivo.Keywords: optical imaging, dendritic cell, superparamagnetic iron oxide, cell trackingSu HMou YBAn YLHan WHuang XFXia GHNi YHZhang YMa JMHu QGDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss Issue 1, Pp 3737-3744 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Su H Mou YB An YL Han W Huang XF Xia GH Ni YH Zhang Y Ma JM Hu QG The migration of synthetic magnetic nanoparticle labeled dendritic cells into lymph nodes with optical imaging
description	Hang Su,1,* Yongbin Mou,1,* Yanli An,2 Wei Han,1 Xiaofeng Huang,1 Guohua Xia,3 Yanhong Ni,1 Yu Zhang,4 Jianmin Ma,1 Qingang Hu1,5 1Center Laboratory of Stomatology, Stomatological Hospital Affiliated Medical School, Nanjing University, Nanjing, People's Republic of China; 2Jiangsu Key Lab of Molecular and Function Imaging, Department of Radiology; 3Department of Hematology, Zhongda Hospital, Medical School, 4State Key Laboratory of Molecule and Bimolecular Electronics, Jiangsu Provincial Laboratory for Biomaterials and Devices; Southeast University, Nanjing, People's Republic of China; 5Leeds Dental Institute, Faculty of Medicine and health, University of Leeds, Leeds, United Kingdom*These authors contributed equally to this workBackground: The successful biotherapy of carcinoma with dendritic cell (DC) vaccines pivotally relies on DCs’ migratory capability into lymph tissues and activation of T cells. Accurate imaging and evaluation of DC migration in vivo have great significance during antitumor treatment with DC vaccine. We herein examined the behavior of DCs influenced by synthetic superparamagnetic iron oxide (SPIO) nanoparticle labeling.Methods: γ-Fe2O3 nanoparticles were prepared and DCs, which were induced from bone marrow monocytes of enhanced green fluorescent protein (EGFP) transgenic mice, were labeled. The endocytosis of the SPIO, surface molecules, cell apoptosis and fluorescence intensity of EGFP-DCs were displayed by Prussian blue staining and flow cytometry (FCM), respectively. After EGFP-DCs, labeled with SPIO, were injected into footpads (n = 5) for 24 hours, the mice were examined in vivo by optical imaging (OPI). Meanwhile, confocal imaging and FCM were applied, respectively, to detect the migration of labeled DCs into draining lymph nodes.Results: Nearly 100% of cells were labeled by the SPIO, in which the intracellular blue color gradually deepened and the iron contents rose with the increase of labeling iron concentrations. In addition, cell apoptosis and the surface molecules on DCs were at similar levels after SPIO labeling. After confirming that the fluorescence intensity of EGFP on DCs was not influenced by SPIO, the homing ability of EGFP-DCs labeled with SPIO displayed that the fluorescence intensity and the ratios of EGFP-DCs in draining lymph nodes were gradually decreased with the increase of labeling iron concentrations.Conclusion: The synthetic SPIO nanoparticles possess perfect labeling ability and biocompatibility. Moreover, DCs labeled with a low dose of SPIO showed stronger migratory capability in vivo.Keywords: optical imaging, dendritic cell, superparamagnetic iron oxide, cell tracking
format	article
author	Su H Mou YB An YL Han W Huang XF Xia GH Ni YH Zhang Y Ma JM Hu QG
author_facet	Su H Mou YB An YL Han W Huang XF Xia GH Ni YH Zhang Y Ma JM Hu QG
author_sort	Su H
title	The migration of synthetic magnetic nanoparticle labeled dendritic cells into lymph nodes with optical imaging
title_short	The migration of synthetic magnetic nanoparticle labeled dendritic cells into lymph nodes with optical imaging
title_full	The migration of synthetic magnetic nanoparticle labeled dendritic cells into lymph nodes with optical imaging
title_fullStr	The migration of synthetic magnetic nanoparticle labeled dendritic cells into lymph nodes with optical imaging
title_full_unstemmed	The migration of synthetic magnetic nanoparticle labeled dendritic cells into lymph nodes with optical imaging
title_sort	migration of synthetic magnetic nanoparticle labeled dendritic cells into lymph nodes with optical imaging
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/3c0cae2aa9ee49b481dcaed4bb0e503a
work_keys_str_mv	AT suh themigrationofsyntheticmagneticnanoparticlelabeleddendriticcellsintolymphnodeswithopticalimaging AT mouyb themigrationofsyntheticmagneticnanoparticlelabeleddendriticcellsintolymphnodeswithopticalimaging AT anyl themigrationofsyntheticmagneticnanoparticlelabeleddendriticcellsintolymphnodeswithopticalimaging AT hanw themigrationofsyntheticmagneticnanoparticlelabeleddendriticcellsintolymphnodeswithopticalimaging AT huangxf themigrationofsyntheticmagneticnanoparticlelabeleddendriticcellsintolymphnodeswithopticalimaging AT xiagh themigrationofsyntheticmagneticnanoparticlelabeleddendriticcellsintolymphnodeswithopticalimaging AT niyh themigrationofsyntheticmagneticnanoparticlelabeleddendriticcellsintolymphnodeswithopticalimaging AT zhangy themigrationofsyntheticmagneticnanoparticlelabeleddendriticcellsintolymphnodeswithopticalimaging AT majm themigrationofsyntheticmagneticnanoparticlelabeleddendriticcellsintolymphnodeswithopticalimaging AT huqg themigrationofsyntheticmagneticnanoparticlelabeleddendriticcellsintolymphnodeswithopticalimaging AT suh migrationofsyntheticmagneticnanoparticlelabeleddendriticcellsintolymphnodeswithopticalimaging AT mouyb migrationofsyntheticmagneticnanoparticlelabeleddendriticcellsintolymphnodeswithopticalimaging AT anyl migrationofsyntheticmagneticnanoparticlelabeleddendriticcellsintolymphnodeswithopticalimaging AT hanw migrationofsyntheticmagneticnanoparticlelabeleddendriticcellsintolymphnodeswithopticalimaging AT huangxf migrationofsyntheticmagneticnanoparticlelabeleddendriticcellsintolymphnodeswithopticalimaging AT xiagh migrationofsyntheticmagneticnanoparticlelabeleddendriticcellsintolymphnodeswithopticalimaging AT niyh migrationofsyntheticmagneticnanoparticlelabeleddendriticcellsintolymphnodeswithopticalimaging AT zhangy migrationofsyntheticmagneticnanoparticlelabeleddendriticcellsintolymphnodeswithopticalimaging AT majm migrationofsyntheticmagneticnanoparticlelabeleddendriticcellsintolymphnodeswithopticalimaging AT huqg migrationofsyntheticmagneticnanoparticlelabeleddendriticcellsintolymphnodeswithopticalimaging
_version_	1718399512709431296

The migration of synthetic magnetic nanoparticle labeled dendritic cells into lymph nodes with optical imaging

Ejemplares similares