Cloning, characterization and analysis of cat and ben genes from the phenol degrading halophilic bacterium Halomonas organivorans.

<h4>Background</h4>Extensive use of phenolic compounds in industry has resulted in the generation of saline wastewaters that produce significant environmental contamination; however, little information is available on the degradation of phenolic compounds in saline conditions. Halomonas...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Maria de Lourdes Moreno, Cristina Sánchez-Porro, Francine Piubeli, Luciana Frias, María Teresa García, Encarnación Mellado
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3c0d41a829dc4c66975c22b31c2d5b0c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3c0d41a829dc4c66975c22b31c2d5b0c
record_format	dspace
spelling	oai:doaj.org-article:3c0d41a829dc4c66975c22b31c2d5b0c2021-11-18T06:52:14ZCloning, characterization and analysis of cat and ben genes from the phenol degrading halophilic bacterium Halomonas organivorans.1932-620310.1371/journal.pone.0021049https://doaj.org/article/3c0d41a829dc4c66975c22b31c2d5b0c2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21695219/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Extensive use of phenolic compounds in industry has resulted in the generation of saline wastewaters that produce significant environmental contamination; however, little information is available on the degradation of phenolic compounds in saline conditions. Halomonas organivorans G-16.1 (CECT 5995(T)) is a moderately halophilic bacterium that we isolated in a previous work from saline environments of South Spain by enrichment for growth in different pollutants, including phenolic compounds. PCR amplification with degenerate primers revealed the presence of genes encoding ring-cleaving enzymes of the β-ketoadipate pathway for aromatic catabolism in H. organivorans.<h4>Findings</h4>The gene cluster catRBCA, involved in catechol degradation, was isolated from H. organivorans. The genes catA, catB, catC and the divergently transcribed catR code for catechol 1,2-dioxygenase (1,2-CTD), cis,cis-muconate cycloisomerase, muconolactone delta-isomerase and a LysR-type transcriptional regulator, respectively. The benzoate catabolic genes (benA and benB) are located flanking the cat genes. The expression of cat and ben genes by phenol and benzoic acid was shown by RT-PCR analysis. The induction of catA gene by phenol and benzoic acid was also probed by the measurement of 1,2-CTD activity in H. organivorans growth in presence of these inducers. 16S rRNA and catA gene-based phylogenies were established among different degrading bacteria showing no phylogenetic correlation between both genes.<h4>Conclusions/significance</h4>In this work, we isolated and determined the sequence of a gene cluster from a moderately halophilic bacterium encoding ortho-pathway genes involved in the catabolic metabolism of phenol and analyzed the gene organization, constituting the first report characterizing catabolic genes involved in the degradation of phenol in moderate halophiles, providing an ideal model system to investigate the potential use of this group of extremophiles in the decontamination of saline environments.Maria de Lourdes MorenoCristina Sánchez-PorroFrancine PiubeliLuciana FriasMaría Teresa GarcíaEncarnación MelladoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 6, p e21049 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maria de Lourdes Moreno Cristina Sánchez-Porro Francine Piubeli Luciana Frias María Teresa García Encarnación Mellado Cloning, characterization and analysis of cat and ben genes from the phenol degrading halophilic bacterium Halomonas organivorans.
description	<h4>Background</h4>Extensive use of phenolic compounds in industry has resulted in the generation of saline wastewaters that produce significant environmental contamination; however, little information is available on the degradation of phenolic compounds in saline conditions. Halomonas organivorans G-16.1 (CECT 5995(T)) is a moderately halophilic bacterium that we isolated in a previous work from saline environments of South Spain by enrichment for growth in different pollutants, including phenolic compounds. PCR amplification with degenerate primers revealed the presence of genes encoding ring-cleaving enzymes of the β-ketoadipate pathway for aromatic catabolism in H. organivorans.<h4>Findings</h4>The gene cluster catRBCA, involved in catechol degradation, was isolated from H. organivorans. The genes catA, catB, catC and the divergently transcribed catR code for catechol 1,2-dioxygenase (1,2-CTD), cis,cis-muconate cycloisomerase, muconolactone delta-isomerase and a LysR-type transcriptional regulator, respectively. The benzoate catabolic genes (benA and benB) are located flanking the cat genes. The expression of cat and ben genes by phenol and benzoic acid was shown by RT-PCR analysis. The induction of catA gene by phenol and benzoic acid was also probed by the measurement of 1,2-CTD activity in H. organivorans growth in presence of these inducers. 16S rRNA and catA gene-based phylogenies were established among different degrading bacteria showing no phylogenetic correlation between both genes.<h4>Conclusions/significance</h4>In this work, we isolated and determined the sequence of a gene cluster from a moderately halophilic bacterium encoding ortho-pathway genes involved in the catabolic metabolism of phenol and analyzed the gene organization, constituting the first report characterizing catabolic genes involved in the degradation of phenol in moderate halophiles, providing an ideal model system to investigate the potential use of this group of extremophiles in the decontamination of saline environments.
format	article
author	Maria de Lourdes Moreno Cristina Sánchez-Porro Francine Piubeli Luciana Frias María Teresa García Encarnación Mellado
author_facet	Maria de Lourdes Moreno Cristina Sánchez-Porro Francine Piubeli Luciana Frias María Teresa García Encarnación Mellado
author_sort	Maria de Lourdes Moreno
title	Cloning, characterization and analysis of cat and ben genes from the phenol degrading halophilic bacterium Halomonas organivorans.
title_short	Cloning, characterization and analysis of cat and ben genes from the phenol degrading halophilic bacterium Halomonas organivorans.
title_full	Cloning, characterization and analysis of cat and ben genes from the phenol degrading halophilic bacterium Halomonas organivorans.
title_fullStr	Cloning, characterization and analysis of cat and ben genes from the phenol degrading halophilic bacterium Halomonas organivorans.
title_full_unstemmed	Cloning, characterization and analysis of cat and ben genes from the phenol degrading halophilic bacterium Halomonas organivorans.
title_sort	cloning, characterization and analysis of cat and ben genes from the phenol degrading halophilic bacterium halomonas organivorans.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/3c0d41a829dc4c66975c22b31c2d5b0c
work_keys_str_mv	AT mariadelourdesmoreno cloningcharacterizationandanalysisofcatandbengenesfromthephenoldegradinghalophilicbacteriumhalomonasorganivorans AT cristinasanchezporro cloningcharacterizationandanalysisofcatandbengenesfromthephenoldegradinghalophilicbacteriumhalomonasorganivorans AT francinepiubeli cloningcharacterizationandanalysisofcatandbengenesfromthephenoldegradinghalophilicbacteriumhalomonasorganivorans AT lucianafrias cloningcharacterizationandanalysisofcatandbengenesfromthephenoldegradinghalophilicbacteriumhalomonasorganivorans AT mariateresagarcia cloningcharacterizationandanalysisofcatandbengenesfromthephenoldegradinghalophilicbacteriumhalomonasorganivorans AT encarnacionmellado cloningcharacterizationandanalysisofcatandbengenesfromthephenoldegradinghalophilicbacteriumhalomonasorganivorans
_version_	1718424285327917056

Cloning, characterization and analysis of cat and ben genes from the phenol degrading halophilic bacterium Halomonas organivorans.

Ejemplares similares