Improvement of Heart Function After Transplantation of Encapsulated Stem Cells Induced with miR-1/Myocd in Myocardial Infarction Model of Rat

Cardiovascular disease is one of the most common causes of death worldwide. Mesenchymal stem cells (MSCs) are one of the most common sources in cell-based therapies in heart regeneration. There are several methods to differentiate MSCs into cardiac-like cells, such as gene induction. Moreover, using...

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Autores principales:	Samaneh Khazaei, Masoud Soleimani, Seyed Hossein Ahmadi Tafti, Rouhollah Mehdinavaz Aghdam, Zohreh Hojati
Formato:	article
Lenguaje:	EN
Publicado:	SAGE Publishing 2021
Materias:	Medicine R
Acceso en línea:	https://doaj.org/article/3c0e147b13dc48c4b9c9b980926915c6
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spelling	oai:doaj.org-article:3c0e147b13dc48c4b9c9b980926915c62021-11-12T08:03:20ZImprovement of Heart Function After Transplantation of Encapsulated Stem Cells Induced with miR-1/Myocd in Myocardial Infarction Model of Rat1555-389210.1177/09636897211048786https://doaj.org/article/3c0e147b13dc48c4b9c9b980926915c62021-10-01T00:00:00Zhttps://doi.org/10.1177/09636897211048786https://doaj.org/toc/1555-3892Cardiovascular disease is one of the most common causes of death worldwide. Mesenchymal stem cells (MSCs) are one of the most common sources in cell-based therapies in heart regeneration. There are several methods to differentiate MSCs into cardiac-like cells, such as gene induction. Moreover, using a three-dimensional (3D) culture, such as hydrogels increases efficiency of differentiation. In the current study, mouse adipose-derived MSCs were co-transduced with lentiviruses containing microRNA-1 ( miR-1 ) and Myocardin ( Myocd ). Then, expression of cardiac markers, such as NK2 homeobox 5( Nkx2-5 ), GATA binding protein 4 ( Gata4 ), and troponin T type 2 ( Tnnt2 ) was investigated, at both gene and protein levels in two-dimensional (2D) culture and chitosan/collagen hydrogel (CS/CO) as a 3D culture. Additionally, after induction of myocardial infarction (MI) in rats, a patch containing the encapsulated induced cardiomyocytes (iCM/P) was implanted to MI zone. Subsequently, 30 days after MI induction, echocardiography, immunohistochemistry staining, and histological examination were performed to evaluate cardiac function. The results of quantitative real -time polymerase chain reaction (qRT-PCR) and immunocytochemistry showed that co-induction of miR-1 and Myocd in MSCs followed by 3D culture of transduced cells increased expression of cardiac markers. Besides, results of in vivo study implicated that heart function was improved in MI model of rats in iCM/P-treated group. The results suggested that miR-1/Myocd induction combined with encapsulation of transduced cells in CS/CO hydrogel increased efficiency of MSCs differentiation into iCMs and could improve heart function in MI model of rats after implantation.Samaneh KhazaeiMasoud SoleimaniSeyed Hossein Ahmadi TaftiRouhollah Mehdinavaz AghdamZohreh HojatiSAGE PublishingarticleMedicineRENCell Transplantation, Vol 30 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R
spellingShingle	Medicine R Samaneh Khazaei Masoud Soleimani Seyed Hossein Ahmadi Tafti Rouhollah Mehdinavaz Aghdam Zohreh Hojati Improvement of Heart Function After Transplantation of Encapsulated Stem Cells Induced with miR-1/Myocd in Myocardial Infarction Model of Rat
description	Cardiovascular disease is one of the most common causes of death worldwide. Mesenchymal stem cells (MSCs) are one of the most common sources in cell-based therapies in heart regeneration. There are several methods to differentiate MSCs into cardiac-like cells, such as gene induction. Moreover, using a three-dimensional (3D) culture, such as hydrogels increases efficiency of differentiation. In the current study, mouse adipose-derived MSCs were co-transduced with lentiviruses containing microRNA-1 ( miR-1 ) and Myocardin ( Myocd ). Then, expression of cardiac markers, such as NK2 homeobox 5( Nkx2-5 ), GATA binding protein 4 ( Gata4 ), and troponin T type 2 ( Tnnt2 ) was investigated, at both gene and protein levels in two-dimensional (2D) culture and chitosan/collagen hydrogel (CS/CO) as a 3D culture. Additionally, after induction of myocardial infarction (MI) in rats, a patch containing the encapsulated induced cardiomyocytes (iCM/P) was implanted to MI zone. Subsequently, 30 days after MI induction, echocardiography, immunohistochemistry staining, and histological examination were performed to evaluate cardiac function. The results of quantitative real -time polymerase chain reaction (qRT-PCR) and immunocytochemistry showed that co-induction of miR-1 and Myocd in MSCs followed by 3D culture of transduced cells increased expression of cardiac markers. Besides, results of in vivo study implicated that heart function was improved in MI model of rats in iCM/P-treated group. The results suggested that miR-1/Myocd induction combined with encapsulation of transduced cells in CS/CO hydrogel increased efficiency of MSCs differentiation into iCMs and could improve heart function in MI model of rats after implantation.
format	article
author	Samaneh Khazaei Masoud Soleimani Seyed Hossein Ahmadi Tafti Rouhollah Mehdinavaz Aghdam Zohreh Hojati
author_facet	Samaneh Khazaei Masoud Soleimani Seyed Hossein Ahmadi Tafti Rouhollah Mehdinavaz Aghdam Zohreh Hojati
author_sort	Samaneh Khazaei
title	Improvement of Heart Function After Transplantation of Encapsulated Stem Cells Induced with miR-1/Myocd in Myocardial Infarction Model of Rat
title_short	Improvement of Heart Function After Transplantation of Encapsulated Stem Cells Induced with miR-1/Myocd in Myocardial Infarction Model of Rat
title_full	Improvement of Heart Function After Transplantation of Encapsulated Stem Cells Induced with miR-1/Myocd in Myocardial Infarction Model of Rat
title_fullStr	Improvement of Heart Function After Transplantation of Encapsulated Stem Cells Induced with miR-1/Myocd in Myocardial Infarction Model of Rat
title_full_unstemmed	Improvement of Heart Function After Transplantation of Encapsulated Stem Cells Induced with miR-1/Myocd in Myocardial Infarction Model of Rat
title_sort	improvement of heart function after transplantation of encapsulated stem cells induced with mir-1/myocd in myocardial infarction model of rat
publisher	SAGE Publishing
publishDate	2021
url	https://doaj.org/article/3c0e147b13dc48c4b9c9b980926915c6
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Improvement of Heart Function After Transplantation of Encapsulated Stem Cells Induced with miR-1/Myocd in Myocardial Infarction Model of Rat

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