The critical role of natural history museums in advancing eDNA for biodiversity studies: a case study with Amazonian fishes

Abstract Ichthyological surveys have traditionally been conducted using whole-specimen, capture-based sampling with varied but conventional fishing gear. Recently, environmental DNA (eDNA) metabarcoding has emerged as a complementary, and possible alternative, approach to whole-specimen methodologie...

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Autores principales:	C. David de Santana, Lynne R. Parenti, Casey B. Dillman, Jonathan A. Coddington, Douglas A. Bastos, Carole C. Baldwin, Jansen Zuanon, Gislene Torrente-Vilara, Raphaël Covain, Naércio A. Menezes, Aléssio Datovo, T. Sado, M. Miya
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3c11a74e99d149e28d16aa6ba591f9e7
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spelling	oai:doaj.org-article:3c11a74e99d149e28d16aa6ba591f9e72021-12-02T17:25:43ZThe critical role of natural history museums in advancing eDNA for biodiversity studies: a case study with Amazonian fishes10.1038/s41598-021-97128-32045-2322https://doaj.org/article/3c11a74e99d149e28d16aa6ba591f9e72021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97128-3https://doaj.org/toc/2045-2322Abstract Ichthyological surveys have traditionally been conducted using whole-specimen, capture-based sampling with varied but conventional fishing gear. Recently, environmental DNA (eDNA) metabarcoding has emerged as a complementary, and possible alternative, approach to whole-specimen methodologies. In the tropics, where much of the diversity remains undescribed, vast reaches continue unexplored, and anthropogenic activities are constant threats; there have been few eDNA attempts for ichthyological inventories. We tested the discriminatory power of eDNA using MiFish primers with existing public reference libraries and compared this with capture-based methods in two distinct ecosystems in the megadiverse Amazon basin. In our study, eDNA provided an accurate snapshot of the fishes at higher taxonomic levels and corroborated its effectiveness to detect specialized fish assemblages. Some flaws in fish metabarcoding studies are routine issues addressed in natural history museums. Thus, by expanding their archives and adopting a series of initiatives linking collection-based research, training and outreach, natural history museums can enable the effective use of eDNA to survey Earth’s hotspots of biodiversity before taxa go extinct. Our project surveying poorly explored rivers and using DNA vouchered archives to build metabarcoding libraries for Neotropical fishes can serve as a model of this protocol.C. David de SantanaLynne R. ParentiCasey B. DillmanJonathan A. CoddingtonDouglas A. BastosCarole C. BaldwinJansen ZuanonGislene Torrente-VilaraRaphaël CovainNaércio A. MenezesAléssio DatovoT. SadoM. MiyaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-22 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q C. David de Santana Lynne R. Parenti Casey B. Dillman Jonathan A. Coddington Douglas A. Bastos Carole C. Baldwin Jansen Zuanon Gislene Torrente-Vilara Raphaël Covain Naércio A. Menezes Aléssio Datovo T. Sado M. Miya The critical role of natural history museums in advancing eDNA for biodiversity studies: a case study with Amazonian fishes
description	Abstract Ichthyological surveys have traditionally been conducted using whole-specimen, capture-based sampling with varied but conventional fishing gear. Recently, environmental DNA (eDNA) metabarcoding has emerged as a complementary, and possible alternative, approach to whole-specimen methodologies. In the tropics, where much of the diversity remains undescribed, vast reaches continue unexplored, and anthropogenic activities are constant threats; there have been few eDNA attempts for ichthyological inventories. We tested the discriminatory power of eDNA using MiFish primers with existing public reference libraries and compared this with capture-based methods in two distinct ecosystems in the megadiverse Amazon basin. In our study, eDNA provided an accurate snapshot of the fishes at higher taxonomic levels and corroborated its effectiveness to detect specialized fish assemblages. Some flaws in fish metabarcoding studies are routine issues addressed in natural history museums. Thus, by expanding their archives and adopting a series of initiatives linking collection-based research, training and outreach, natural history museums can enable the effective use of eDNA to survey Earth’s hotspots of biodiversity before taxa go extinct. Our project surveying poorly explored rivers and using DNA vouchered archives to build metabarcoding libraries for Neotropical fishes can serve as a model of this protocol.
format	article
author	C. David de Santana Lynne R. Parenti Casey B. Dillman Jonathan A. Coddington Douglas A. Bastos Carole C. Baldwin Jansen Zuanon Gislene Torrente-Vilara Raphaël Covain Naércio A. Menezes Aléssio Datovo T. Sado M. Miya
author_facet	C. David de Santana Lynne R. Parenti Casey B. Dillman Jonathan A. Coddington Douglas A. Bastos Carole C. Baldwin Jansen Zuanon Gislene Torrente-Vilara Raphaël Covain Naércio A. Menezes Aléssio Datovo T. Sado M. Miya
author_sort	C. David de Santana
title	The critical role of natural history museums in advancing eDNA for biodiversity studies: a case study with Amazonian fishes
title_short	The critical role of natural history museums in advancing eDNA for biodiversity studies: a case study with Amazonian fishes
title_full	The critical role of natural history museums in advancing eDNA for biodiversity studies: a case study with Amazonian fishes
title_fullStr	The critical role of natural history museums in advancing eDNA for biodiversity studies: a case study with Amazonian fishes
title_full_unstemmed	The critical role of natural history museums in advancing eDNA for biodiversity studies: a case study with Amazonian fishes
title_sort	critical role of natural history museums in advancing edna for biodiversity studies: a case study with amazonian fishes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3c11a74e99d149e28d16aa6ba591f9e7
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The critical role of natural history museums in advancing eDNA for biodiversity studies: a case study with Amazonian fishes

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