DNA origami scaffold for studying intrinsically disordered proteins of the nuclear pore complex

DNA origami scaffold for studying intrinsically disordered proteins of the nuclear pore complex

FG-Nups are disordered proteins in the nuclear pore complex (NPC) where they selectively control nuclear transport. Here authors build NPC-mimics based on DNA origami rings which attach a certain numbers of Nups to analyse those nanopores by cryoEM and conductance measurements.

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Autores principales: Philip Ketterer, Adithya N. Ananth, Diederik S. Laman Trip, Ankur Mishra, Eva Bertosin, Mahipal Ganji, Jaco van der Torre, Patrick Onck, Hendrik Dietz, Cees Dekker
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
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Science
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Acceso en línea:https://doaj.org/article/3c16edd0fd33457389226bffa28bd552
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