Engineering the protein dynamics of an ancestral luciferase

Directed evolution commonly relies on point mutations but InDels frequently occur in evolution. Here the authors report a protein-engineering framework based on InDel mutagenesis and fragment transplantation resulting in greater catalysis and longer glow-type bioluminescence of the ancestral lucifer...

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Autores principales:	Andrea Schenkmayerova, Gaspar P. Pinto, Martin Toul, Martin Marek, Lenka Hernychova, Joan Planas-Iglesias, Veronika Daniel Liskova, Daniel Pluskal, Michal Vasina, Stephane Emond, Mark Dörr, Radka Chaloupkova, David Bednar, Zbynek Prokop, Florian Hollfelder, Uwe T. Bornscheuer, Jiri Damborsky
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/3c18b7a5373d42f0b49ea5eb1b1dcbad
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spelling	oai:doaj.org-article:3c18b7a5373d42f0b49ea5eb1b1dcbad2021-12-02T17:24:08ZEngineering the protein dynamics of an ancestral luciferase10.1038/s41467-021-23450-z2041-1723https://doaj.org/article/3c18b7a5373d42f0b49ea5eb1b1dcbad2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-23450-zhttps://doaj.org/toc/2041-1723Directed evolution commonly relies on point mutations but InDels frequently occur in evolution. Here the authors report a protein-engineering framework based on InDel mutagenesis and fragment transplantation resulting in greater catalysis and longer glow-type bioluminescence of the ancestral luciferase.Andrea SchenkmayerovaGaspar P. PintoMartin ToulMartin MarekLenka HernychovaJoan Planas-IglesiasVeronika Daniel LiskovaDaniel PluskalMichal VasinaStephane EmondMark DörrRadka ChaloupkovaDavid BednarZbynek ProkopFlorian HollfelderUwe T. BornscheuerJiri DamborskyNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Andrea Schenkmayerova Gaspar P. Pinto Martin Toul Martin Marek Lenka Hernychova Joan Planas-Iglesias Veronika Daniel Liskova Daniel Pluskal Michal Vasina Stephane Emond Mark Dörr Radka Chaloupkova David Bednar Zbynek Prokop Florian Hollfelder Uwe T. Bornscheuer Jiri Damborsky Engineering the protein dynamics of an ancestral luciferase
description	Directed evolution commonly relies on point mutations but InDels frequently occur in evolution. Here the authors report a protein-engineering framework based on InDel mutagenesis and fragment transplantation resulting in greater catalysis and longer glow-type bioluminescence of the ancestral luciferase.
format	article
author	Andrea Schenkmayerova Gaspar P. Pinto Martin Toul Martin Marek Lenka Hernychova Joan Planas-Iglesias Veronika Daniel Liskova Daniel Pluskal Michal Vasina Stephane Emond Mark Dörr Radka Chaloupkova David Bednar Zbynek Prokop Florian Hollfelder Uwe T. Bornscheuer Jiri Damborsky
author_facet	Andrea Schenkmayerova Gaspar P. Pinto Martin Toul Martin Marek Lenka Hernychova Joan Planas-Iglesias Veronika Daniel Liskova Daniel Pluskal Michal Vasina Stephane Emond Mark Dörr Radka Chaloupkova David Bednar Zbynek Prokop Florian Hollfelder Uwe T. Bornscheuer Jiri Damborsky
author_sort	Andrea Schenkmayerova
title	Engineering the protein dynamics of an ancestral luciferase
title_short	Engineering the protein dynamics of an ancestral luciferase
title_full	Engineering the protein dynamics of an ancestral luciferase
title_fullStr	Engineering the protein dynamics of an ancestral luciferase
title_full_unstemmed	Engineering the protein dynamics of an ancestral luciferase
title_sort	engineering the protein dynamics of an ancestral luciferase
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3c18b7a5373d42f0b49ea5eb1b1dcbad
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Engineering the protein dynamics of an ancestral luciferase

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