l-Lactic Acid Production Using Engineered Saccharomyces cerevisiae with Improved Organic Acid Tolerance

Lactic acid is mainly used to produce bio-based, bio-degradable polylactic acid. For industrial production of lactic acid, engineered Saccharomyces cerevisiae can be used. To avoid cellular toxicity caused by lactic acid accumulation, pH-neutralizing agents are used, leading to in...

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Autores principales:	Byeong-Kwan Jang, Yebin Ju, Deokyeol Jeong, Sung-Keun Jung, Chang-Kil Kim, Yong-Suk Chung, Soo-Rin Kim
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	lactate dehydrogenase whole-genome sequencing <i>Saccharomyces cerevisiae</i> lignocellulosic biomass polylactic acid Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3c1c44d3bdf0497c9cbd8c762849c684
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id	oai:doaj.org-article:3c1c44d3bdf0497c9cbd8c762849c684
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spelling	oai:doaj.org-article:3c1c44d3bdf0497c9cbd8c762849c6842021-11-25T18:05:51Z<span style="font-variant: small-caps">l</span>-Lactic Acid Production Using Engineered <i>Saccharomyces cerevisiae</i> with Improved Organic Acid Tolerance10.3390/jof71109282309-608Xhttps://doaj.org/article/3c1c44d3bdf0497c9cbd8c762849c6842021-10-01T00:00:00Zhttps://www.mdpi.com/2309-608X/7/11/928https://doaj.org/toc/2309-608XLactic acid is mainly used to produce bio-based, bio-degradable polylactic acid. For industrial production of lactic acid, engineered <i>Saccharomyces cerevisiae</i> can be used. To avoid cellular toxicity caused by lactic acid accumulation, pH-neutralizing agents are used, leading to increased production costs. In this study, lactic acid-producing <i>S. cerevisiae</i> BK01 was developed with improved lactic acid tolerance through adaptive laboratory evolution (ALE) on 8% lactic acid. The genetic basis of BK01 could not be determined, suggesting complex mechanisms associated with lactic acid tolerance. However, BK01 had distinctive metabolomic traits clearly separated from the parental strain, and lactic acid production was improved by 17% (from 102 g/L to 119 g/L). To the best of our knowledge, this is the highest lactic acid titer produced by engineered <i>S. cerevisiae</i> without the use of pH neutralizers. Moreover, cellulosic lactic acid production by BK01 was demonstrated using acetate-rich buckwheat husk hydrolysates. Particularly, BK01 revealed improved tolerance against acetic acid of the hydrolysates, a major fermentation inhibitor of lignocellulosic biomass. In short, ALE with a high concentration of lactic acid improved lactic acid production as well as acetic acid tolerance of BK01, suggesting a potential for economically viable cellulosic lactic acid production.Byeong-Kwan JangYebin JuDeokyeol JeongSung-Keun JungChang-Kil KimYong-Suk ChungSoo-Rin KimMDPI AGarticlelactate dehydrogenasewhole-genome sequencing<i>Saccharomyces cerevisiae</i>lignocellulosic biomasspolylactic acidBiology (General)QH301-705.5ENJournal of Fungi, Vol 7, Iss 928, p 928 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lactate dehydrogenase whole-genome sequencing <i>Saccharomyces cerevisiae</i> lignocellulosic biomass polylactic acid Biology (General) QH301-705.5
spellingShingle	lactate dehydrogenase whole-genome sequencing <i>Saccharomyces cerevisiae</i> lignocellulosic biomass polylactic acid Biology (General) QH301-705.5 Byeong-Kwan Jang Yebin Ju Deokyeol Jeong Sung-Keun Jung Chang-Kil Kim Yong-Suk Chung Soo-Rin Kim <span style="font-variant: small-caps">l</span>-Lactic Acid Production Using Engineered <i>Saccharomyces cerevisiae</i> with Improved Organic Acid Tolerance
description	Lactic acid is mainly used to produce bio-based, bio-degradable polylactic acid. For industrial production of lactic acid, engineered <i>Saccharomyces cerevisiae</i> can be used. To avoid cellular toxicity caused by lactic acid accumulation, pH-neutralizing agents are used, leading to increased production costs. In this study, lactic acid-producing <i>S. cerevisiae</i> BK01 was developed with improved lactic acid tolerance through adaptive laboratory evolution (ALE) on 8% lactic acid. The genetic basis of BK01 could not be determined, suggesting complex mechanisms associated with lactic acid tolerance. However, BK01 had distinctive metabolomic traits clearly separated from the parental strain, and lactic acid production was improved by 17% (from 102 g/L to 119 g/L). To the best of our knowledge, this is the highest lactic acid titer produced by engineered <i>S. cerevisiae</i> without the use of pH neutralizers. Moreover, cellulosic lactic acid production by BK01 was demonstrated using acetate-rich buckwheat husk hydrolysates. Particularly, BK01 revealed improved tolerance against acetic acid of the hydrolysates, a major fermentation inhibitor of lignocellulosic biomass. In short, ALE with a high concentration of lactic acid improved lactic acid production as well as acetic acid tolerance of BK01, suggesting a potential for economically viable cellulosic lactic acid production.
format	article
author	Byeong-Kwan Jang Yebin Ju Deokyeol Jeong Sung-Keun Jung Chang-Kil Kim Yong-Suk Chung Soo-Rin Kim
author_facet	Byeong-Kwan Jang Yebin Ju Deokyeol Jeong Sung-Keun Jung Chang-Kil Kim Yong-Suk Chung Soo-Rin Kim
author_sort	Byeong-Kwan Jang
title	<span style="font-variant: small-caps">l</span>-Lactic Acid Production Using Engineered <i>Saccharomyces cerevisiae</i> with Improved Organic Acid Tolerance
title_short	<span style="font-variant: small-caps">l</span>-Lactic Acid Production Using Engineered <i>Saccharomyces cerevisiae</i> with Improved Organic Acid Tolerance
title_full	<span style="font-variant: small-caps">l</span>-Lactic Acid Production Using Engineered <i>Saccharomyces cerevisiae</i> with Improved Organic Acid Tolerance
title_fullStr	<span style="font-variant: small-caps">l</span>-Lactic Acid Production Using Engineered <i>Saccharomyces cerevisiae</i> with Improved Organic Acid Tolerance
title_full_unstemmed	<span style="font-variant: small-caps">l</span>-Lactic Acid Production Using Engineered <i>Saccharomyces cerevisiae</i> with Improved Organic Acid Tolerance
title_sort	<span style="font-variant: small-caps">l</span>-lactic acid production using engineered <i>saccharomyces cerevisiae</i> with improved organic acid tolerance
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3c1c44d3bdf0497c9cbd8c762849c684
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<span style="font-variant: small-caps">l</span>-Lactic Acid Production Using Engineered <i>Saccharomyces cerevisiae</i> with Improved Organic Acid Tolerance

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