Beneficial Chromosomal Integration of the Genes for CTX-M Extended-Spectrum β-Lactamase in <italic toggle="yes">Klebsiella pneumoniae</italic> for Stable Propagation

ABSTRACT The acquired CTX-M-type extended-spectrum-β-lactamase (ESBL)-producing Enterobacterales are of great concern in clinical settings because they limit therapeutic options for patients infected by the pathogens. An intriguing clonality of CTX-M ESBL-producing Klebsiella pneumoniae blood isolat...

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Autores principales:	Eun-Jeong Yoon, Bareum Gwon, Changseung Liu, Dokyun Kim, Dongju Won, Sung Gyun Park, Jong Rak Choi, Seok Hoon Jeong
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	CTX-M extended-spectrum β-lactamases Klebsiella pneumoniae chromosomal integration ISEcp1 IS26 Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/3c26f53c45bc42ee901a79976e808057
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spelling	oai:doaj.org-article:3c26f53c45bc42ee901a79976e8080572021-12-02T19:46:19ZBeneficial Chromosomal Integration of the Genes for CTX-M Extended-Spectrum β-Lactamase in <italic toggle="yes">Klebsiella pneumoniae</italic> for Stable Propagation10.1128/mSystems.00459-202379-5077https://doaj.org/article/3c26f53c45bc42ee901a79976e8080572020-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00459-20https://doaj.org/toc/2379-5077ABSTRACT The acquired CTX-M-type extended-spectrum-β-lactamase (ESBL)-producing Enterobacterales are of great concern in clinical settings because they limit therapeutic options for patients infected by the pathogens. An intriguing clonality of CTX-M ESBL-producing Klebsiella pneumoniae blood isolates was observed from a national cohort study, and comparative genomics were assessed for the 115 K. pneumoniae blood isolates carrying the blaCTX-M gene. The plasmid preference of particular clones of a sequence type (ST) was assessed by liquid mating. A quarter of the blaCTX-M gene-carrying K. pneumoniae blood isolates harbor the gene in their chromosome, and most of those with the built-in blaCTX-M gene belonged either to ST307 or ST48. Notably, all 16 K. pneumoniae ST48 isolates harbored two copies of the blaCTX-M-15 gene in the chromosome. The chromosomal integration of the blaCTX-M-15 gene was mostly derived from the ISEcp1-targeting 5-bp AT-rich locus in the chromosome. The IS26-mediated chromosomal integration occurred when the upstream ISEcp1 from the blaCTX-M gene was truncated, targeting the anchor IS26 copy in the chromosome. Higher transfer efficiency of the blaCTX-M-15 gene-carrying FIA:R plasmid was observed in ST17 than that of the blaCTX-M-14 gene-carrying FIB:FII plasmid. The transfer efficiency of the plasmid differed by isolate among the ST307 members. The K. pneumoniae clones ST307 and ST48 harboring the blaCTX-M-15 gene in the chromosome were able to disseminate stably in clinical settings regardless of the environmental pressure, and the current population of K. pneumoniae blood isolates was constructed. Further follow-up is needed for the epidemiology of this antimicrobial resistance. IMPORTANCE Dominant F-type plasmids harboring the gene have been pointed out to be responsible for the dissemination of the CTX-M extended-spectrum-β-lactamase (ESBL)-producing K. pneumoniae. Recently, the emergence of K. pneumoniae isolates with the blaCTX-M gene in their chromosomes has been reported occasionally worldwide. Such a chromosomal location of the resistance gene could be beneficial for stable propagation, as was the Acinetobacter baumannii ST191 harboring chromosomal blaOXA-23 that is endemic to South Korea. Through the present study, particular clones were identified as having built-in resistance genes in their chromosomes, and the chromosomal integration events were tracked by assessing their genomes. The cefotaxime-resistant K. pneumoniae clones of this study were particularized as results of the fastidiousness for plasmids to acquire the blaCTX-M gene for securing the diversity and of the chromosomal addiction of the blaCTX-M gene for ensuring propagation.Eun-Jeong YoonBareum GwonChangseung LiuDokyun KimDongju WonSung Gyun ParkJong Rak ChoiSeok Hoon JeongAmerican Society for MicrobiologyarticleCTX-Mextended-spectrum β-lactamasesKlebsiella pneumoniaechromosomal integrationISEcp1IS26MicrobiologyQR1-502ENmSystems, Vol 5, Iss 5 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	CTX-M extended-spectrum β-lactamases Klebsiella pneumoniae chromosomal integration ISEcp1 IS26 Microbiology QR1-502
spellingShingle	CTX-M extended-spectrum β-lactamases Klebsiella pneumoniae chromosomal integration ISEcp1 IS26 Microbiology QR1-502 Eun-Jeong Yoon Bareum Gwon Changseung Liu Dokyun Kim Dongju Won Sung Gyun Park Jong Rak Choi Seok Hoon Jeong Beneficial Chromosomal Integration of the Genes for CTX-M Extended-Spectrum β-Lactamase in <italic toggle="yes">Klebsiella pneumoniae</italic> for Stable Propagation
description	ABSTRACT The acquired CTX-M-type extended-spectrum-β-lactamase (ESBL)-producing Enterobacterales are of great concern in clinical settings because they limit therapeutic options for patients infected by the pathogens. An intriguing clonality of CTX-M ESBL-producing Klebsiella pneumoniae blood isolates was observed from a national cohort study, and comparative genomics were assessed for the 115 K. pneumoniae blood isolates carrying the blaCTX-M gene. The plasmid preference of particular clones of a sequence type (ST) was assessed by liquid mating. A quarter of the blaCTX-M gene-carrying K. pneumoniae blood isolates harbor the gene in their chromosome, and most of those with the built-in blaCTX-M gene belonged either to ST307 or ST48. Notably, all 16 K. pneumoniae ST48 isolates harbored two copies of the blaCTX-M-15 gene in the chromosome. The chromosomal integration of the blaCTX-M-15 gene was mostly derived from the ISEcp1-targeting 5-bp AT-rich locus in the chromosome. The IS26-mediated chromosomal integration occurred when the upstream ISEcp1 from the blaCTX-M gene was truncated, targeting the anchor IS26 copy in the chromosome. Higher transfer efficiency of the blaCTX-M-15 gene-carrying FIA:R plasmid was observed in ST17 than that of the blaCTX-M-14 gene-carrying FIB:FII plasmid. The transfer efficiency of the plasmid differed by isolate among the ST307 members. The K. pneumoniae clones ST307 and ST48 harboring the blaCTX-M-15 gene in the chromosome were able to disseminate stably in clinical settings regardless of the environmental pressure, and the current population of K. pneumoniae blood isolates was constructed. Further follow-up is needed for the epidemiology of this antimicrobial resistance. IMPORTANCE Dominant F-type plasmids harboring the gene have been pointed out to be responsible for the dissemination of the CTX-M extended-spectrum-β-lactamase (ESBL)-producing K. pneumoniae. Recently, the emergence of K. pneumoniae isolates with the blaCTX-M gene in their chromosomes has been reported occasionally worldwide. Such a chromosomal location of the resistance gene could be beneficial for stable propagation, as was the Acinetobacter baumannii ST191 harboring chromosomal blaOXA-23 that is endemic to South Korea. Through the present study, particular clones were identified as having built-in resistance genes in their chromosomes, and the chromosomal integration events were tracked by assessing their genomes. The cefotaxime-resistant K. pneumoniae clones of this study were particularized as results of the fastidiousness for plasmids to acquire the blaCTX-M gene for securing the diversity and of the chromosomal addiction of the blaCTX-M gene for ensuring propagation.
format	article
author	Eun-Jeong Yoon Bareum Gwon Changseung Liu Dokyun Kim Dongju Won Sung Gyun Park Jong Rak Choi Seok Hoon Jeong
author_facet	Eun-Jeong Yoon Bareum Gwon Changseung Liu Dokyun Kim Dongju Won Sung Gyun Park Jong Rak Choi Seok Hoon Jeong
author_sort	Eun-Jeong Yoon
title	Beneficial Chromosomal Integration of the Genes for CTX-M Extended-Spectrum β-Lactamase in <italic toggle="yes">Klebsiella pneumoniae</italic> for Stable Propagation
title_short	Beneficial Chromosomal Integration of the Genes for CTX-M Extended-Spectrum β-Lactamase in <italic toggle="yes">Klebsiella pneumoniae</italic> for Stable Propagation
title_full	Beneficial Chromosomal Integration of the Genes for CTX-M Extended-Spectrum β-Lactamase in <italic toggle="yes">Klebsiella pneumoniae</italic> for Stable Propagation
title_fullStr	Beneficial Chromosomal Integration of the Genes for CTX-M Extended-Spectrum β-Lactamase in <italic toggle="yes">Klebsiella pneumoniae</italic> for Stable Propagation
title_full_unstemmed	Beneficial Chromosomal Integration of the Genes for CTX-M Extended-Spectrum β-Lactamase in <italic toggle="yes">Klebsiella pneumoniae</italic> for Stable Propagation
title_sort	beneficial chromosomal integration of the genes for ctx-m extended-spectrum β-lactamase in <italic toggle="yes">klebsiella pneumoniae</italic> for stable propagation
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/3c26f53c45bc42ee901a79976e808057
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Beneficial Chromosomal Integration of the Genes for CTX-M Extended-Spectrum β-Lactamase in <italic toggle="yes">Klebsiella pneumoniae</italic> for Stable Propagation

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