Modulating bacterial and gut mucosal interactions with engineered biofilm matrix proteins

Abstract Extracellular appendages play a significant role in mediating communication between bacteria and their host. Curli fibers are a class of bacterial fimbria that is highly amenable to engineering. We demonstrate the use of engineered curli fibers to rationally program interactions between bac...

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Autores principales:	Anna M. Duraj-Thatte, Pichet Praveschotinunt, Trevor R. Nash, Frederick R. Ward, Neel S. Joshi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3c2ed7fc000544358e3696a8052ced74
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spelling	oai:doaj.org-article:3c2ed7fc000544358e3696a8052ced742021-12-02T12:32:36ZModulating bacterial and gut mucosal interactions with engineered biofilm matrix proteins10.1038/s41598-018-21834-82045-2322https://doaj.org/article/3c2ed7fc000544358e3696a8052ced742018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-21834-8https://doaj.org/toc/2045-2322Abstract Extracellular appendages play a significant role in mediating communication between bacteria and their host. Curli fibers are a class of bacterial fimbria that is highly amenable to engineering. We demonstrate the use of engineered curli fibers to rationally program interactions between bacteria and components of the mucosal epithelium. Commensal E. coli strains were engineered to produce recombinant curli fibers fused to the trefoil family of human cytokines. Biofilms formed from these strains bound more mucins than those producing wild-type curli fibers, and modulated mucin rheology as well. When treated with bacteria producing the curli-trefoil fusions mammalian cells behaved identically in terms of their migration behavior as when they were treated with the corresponding soluble trefoil factors. Overall, this demonstrates the potential utility of curli fibers as a scaffold for the display of bioactive domains and an untapped approach to rationally modulating host-microbe interactions using bacterial matrix proteins.Anna M. Duraj-ThattePichet PraveschotinuntTrevor R. NashFrederick R. WardNeel S. JoshiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-8 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anna M. Duraj-Thatte Pichet Praveschotinunt Trevor R. Nash Frederick R. Ward Neel S. Joshi Modulating bacterial and gut mucosal interactions with engineered biofilm matrix proteins
description	Abstract Extracellular appendages play a significant role in mediating communication between bacteria and their host. Curli fibers are a class of bacterial fimbria that is highly amenable to engineering. We demonstrate the use of engineered curli fibers to rationally program interactions between bacteria and components of the mucosal epithelium. Commensal E. coli strains were engineered to produce recombinant curli fibers fused to the trefoil family of human cytokines. Biofilms formed from these strains bound more mucins than those producing wild-type curli fibers, and modulated mucin rheology as well. When treated with bacteria producing the curli-trefoil fusions mammalian cells behaved identically in terms of their migration behavior as when they were treated with the corresponding soluble trefoil factors. Overall, this demonstrates the potential utility of curli fibers as a scaffold for the display of bioactive domains and an untapped approach to rationally modulating host-microbe interactions using bacterial matrix proteins.
format	article
author	Anna M. Duraj-Thatte Pichet Praveschotinunt Trevor R. Nash Frederick R. Ward Neel S. Joshi
author_facet	Anna M. Duraj-Thatte Pichet Praveschotinunt Trevor R. Nash Frederick R. Ward Neel S. Joshi
author_sort	Anna M. Duraj-Thatte
title	Modulating bacterial and gut mucosal interactions with engineered biofilm matrix proteins
title_short	Modulating bacterial and gut mucosal interactions with engineered biofilm matrix proteins
title_full	Modulating bacterial and gut mucosal interactions with engineered biofilm matrix proteins
title_fullStr	Modulating bacterial and gut mucosal interactions with engineered biofilm matrix proteins
title_full_unstemmed	Modulating bacterial and gut mucosal interactions with engineered biofilm matrix proteins
title_sort	modulating bacterial and gut mucosal interactions with engineered biofilm matrix proteins
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/3c2ed7fc000544358e3696a8052ced74
work_keys_str_mv	AT annamdurajthatte modulatingbacterialandgutmucosalinteractionswithengineeredbiofilmmatrixproteins AT pichetpraveschotinunt modulatingbacterialandgutmucosalinteractionswithengineeredbiofilmmatrixproteins AT trevorrnash modulatingbacterialandgutmucosalinteractionswithengineeredbiofilmmatrixproteins AT frederickrward modulatingbacterialandgutmucosalinteractionswithengineeredbiofilmmatrixproteins AT neelsjoshi modulatingbacterialandgutmucosalinteractionswithengineeredbiofilmmatrixproteins
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Modulating bacterial and gut mucosal interactions with engineered biofilm matrix proteins

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