Human milk extracellular vesicle miRNA expression and associations with maternal characteristics in a population-based cohort from the Faroe Islands

Abstract Human milk plays a critical role in infant development and health, particularly in cognitive, immune, and cardiometabolic functions. Milk contains extracellular vesicles (EVs) that can transport biologically relevant cargo from mother to infant, including microRNAs (miRNAs). We aimed to cha...

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Autores principales:	Allison Kupsco, Diddier Prada, Damaskini Valvi, Lisa Hu, Maria Skaalum Petersen, Brent Coull, Philippe Grandjean, Pal Weihe, Andrea A. Baccarelli
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3c3110bf9770408fa6bbe24d05b2019c
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spelling	oai:doaj.org-article:3c3110bf9770408fa6bbe24d05b2019c2021-12-02T11:36:36ZHuman milk extracellular vesicle miRNA expression and associations with maternal characteristics in a population-based cohort from the Faroe Islands10.1038/s41598-021-84809-22045-2322https://doaj.org/article/3c3110bf9770408fa6bbe24d05b2019c2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84809-2https://doaj.org/toc/2045-2322Abstract Human milk plays a critical role in infant development and health, particularly in cognitive, immune, and cardiometabolic functions. Milk contains extracellular vesicles (EVs) that can transport biologically relevant cargo from mother to infant, including microRNAs (miRNAs). We aimed to characterize milk EV-miRNA profiles in a human population cohort, assess potential pathways and ontology, and investigate associations with maternal characteristics. We conducted the first study to describe the EV miRNA profile of human milk in 364 mothers from a population-based mother-infant cohort in the Faroe Islands using small RNA sequencing. We detected 1523 miRNAs with ≥ one read in 70% of samples. Using hierarchical clustering, we determined five EV-miRNA clusters, the top three consisting of 15, 27 and 67 miRNAs. Correlation coefficients indicated that the expression of many miRNAs within the top three clusters was highly correlated. Top-cluster human milk EV-miRNAs were involved in pathways enriched for the endocrine system, cellular community, neurodevelopment, and cancers. miRNA expression was associated with time to milk collection post-delivery, maternal body mass index, and maternal smoking, but not maternal parity. Future studies investigating determinants of human EV-miRNAs and associated health outcomes are needed to elucidate the role of human milk EV-miRNAs in health and disease.Allison KupscoDiddier PradaDamaskini ValviLisa HuMaria Skaalum PetersenBrent CoullPhilippe GrandjeanPal WeiheAndrea A. BaccarelliNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Allison Kupsco Diddier Prada Damaskini Valvi Lisa Hu Maria Skaalum Petersen Brent Coull Philippe Grandjean Pal Weihe Andrea A. Baccarelli Human milk extracellular vesicle miRNA expression and associations with maternal characteristics in a population-based cohort from the Faroe Islands
description	Abstract Human milk plays a critical role in infant development and health, particularly in cognitive, immune, and cardiometabolic functions. Milk contains extracellular vesicles (EVs) that can transport biologically relevant cargo from mother to infant, including microRNAs (miRNAs). We aimed to characterize milk EV-miRNA profiles in a human population cohort, assess potential pathways and ontology, and investigate associations with maternal characteristics. We conducted the first study to describe the EV miRNA profile of human milk in 364 mothers from a population-based mother-infant cohort in the Faroe Islands using small RNA sequencing. We detected 1523 miRNAs with ≥ one read in 70% of samples. Using hierarchical clustering, we determined five EV-miRNA clusters, the top three consisting of 15, 27 and 67 miRNAs. Correlation coefficients indicated that the expression of many miRNAs within the top three clusters was highly correlated. Top-cluster human milk EV-miRNAs were involved in pathways enriched for the endocrine system, cellular community, neurodevelopment, and cancers. miRNA expression was associated with time to milk collection post-delivery, maternal body mass index, and maternal smoking, but not maternal parity. Future studies investigating determinants of human EV-miRNAs and associated health outcomes are needed to elucidate the role of human milk EV-miRNAs in health and disease.
format	article
author	Allison Kupsco Diddier Prada Damaskini Valvi Lisa Hu Maria Skaalum Petersen Brent Coull Philippe Grandjean Pal Weihe Andrea A. Baccarelli
author_facet	Allison Kupsco Diddier Prada Damaskini Valvi Lisa Hu Maria Skaalum Petersen Brent Coull Philippe Grandjean Pal Weihe Andrea A. Baccarelli
author_sort	Allison Kupsco
title	Human milk extracellular vesicle miRNA expression and associations with maternal characteristics in a population-based cohort from the Faroe Islands
title_short	Human milk extracellular vesicle miRNA expression and associations with maternal characteristics in a population-based cohort from the Faroe Islands
title_full	Human milk extracellular vesicle miRNA expression and associations with maternal characteristics in a population-based cohort from the Faroe Islands
title_fullStr	Human milk extracellular vesicle miRNA expression and associations with maternal characteristics in a population-based cohort from the Faroe Islands
title_full_unstemmed	Human milk extracellular vesicle miRNA expression and associations with maternal characteristics in a population-based cohort from the Faroe Islands
title_sort	human milk extracellular vesicle mirna expression and associations with maternal characteristics in a population-based cohort from the faroe islands
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3c3110bf9770408fa6bbe24d05b2019c
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Human milk extracellular vesicle miRNA expression and associations with maternal characteristics in a population-based cohort from the Faroe Islands

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