Evaluating the potential of cubosomal nanoparticles for oral delivery of amphotericin B in treating fungal infection

Zhiwen Yang,1,3 Meiwan Chen,2 Muhua Yang,1 Jian Chen,1 Weijun Fang,1 Ping Xu11Department of Pharmacy, Songjiang Hospital Affiliated The First People's Hospital, Shanghai Jiao Tong University, Shanghai, 2State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medic...

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Autores principales:	Yang Z, Chen M, Yang M, Chen J, Fang W, Xu P
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/3c3b818ad0c44b7585e6f76f3af76acd
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spelling	oai:doaj.org-article:3c3b818ad0c44b7585e6f76f3af76acd2021-12-02T07:20:21ZEvaluating the potential of cubosomal nanoparticles for oral delivery of amphotericin B in treating fungal infection1178-2013https://doaj.org/article/3c3b818ad0c44b7585e6f76f3af76acd2014-01-01T00:00:00Zhttp://www.dovepress.com/evaluating-the-potential-of-cubosomal-nanoparticles-for-oral-delivery--a15421https://doaj.org/toc/1178-2013 Zhiwen Yang,1,3 Meiwan Chen,2 Muhua Yang,1 Jian Chen,1 Weijun Fang,1 Ping Xu11Department of Pharmacy, Songjiang Hospital Affiliated The First People's Hospital, Shanghai Jiao Tong University, Shanghai, 2State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, 3Shanghai Songjiang Hospital Affiliated Nanjing Medical University, Nanjing, People's Republic of ChinaAbstract: The oral administration of amphotericin B (AmB) has a major drawback of poor bioavailability. The aim of this study was to investigate the potential of glyceryl monoolein (GMO) cubosomes as lipid nanocarriers to improve the oral efficacy of AmB. Antifungal efficacy was determined in vivo in rats after oral administration, to investigate its therapeutic use. The human colon adenocarcinoma cell line (Caco-2) was used in vitro to evaluate transport across a model of the intestinal barrier. In vivo antifungal results showed that AmB, loaded in GMO cubosomes, could significantly enhance oral efficacy, compared against Fungizone®, and that during a 2 day course of dosage 10 mg/kg the drug reached effective therapeutic concentrations in renal tissue for treating fungal infections. In the Caco-2 transport studies, GMO cubosomes resulted in a significantly larger amount of AmB being transported into Caco-2 cells, via both clathrin- and caveolae-mediated endocytosis, but not macropinocytosis. These results suggest that GMO cubosomes, as lipid nanovectors, could facilitate the oral delivery of AmB.Keywords: glyceryl monoolein cubosomes, oral delivery, amphotericin B, antifungal activity, absorption mechanismYang ZChen MYang MChen JFang WXu PDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 327-336 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Yang Z Chen M Yang M Chen J Fang W Xu P Evaluating the potential of cubosomal nanoparticles for oral delivery of amphotericin B in treating fungal infection
description	Zhiwen Yang,1,3 Meiwan Chen,2 Muhua Yang,1 Jian Chen,1 Weijun Fang,1 Ping Xu11Department of Pharmacy, Songjiang Hospital Affiliated The First People's Hospital, Shanghai Jiao Tong University, Shanghai, 2State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, 3Shanghai Songjiang Hospital Affiliated Nanjing Medical University, Nanjing, People's Republic of ChinaAbstract: The oral administration of amphotericin B (AmB) has a major drawback of poor bioavailability. The aim of this study was to investigate the potential of glyceryl monoolein (GMO) cubosomes as lipid nanocarriers to improve the oral efficacy of AmB. Antifungal efficacy was determined in vivo in rats after oral administration, to investigate its therapeutic use. The human colon adenocarcinoma cell line (Caco-2) was used in vitro to evaluate transport across a model of the intestinal barrier. In vivo antifungal results showed that AmB, loaded in GMO cubosomes, could significantly enhance oral efficacy, compared against Fungizone®, and that during a 2 day course of dosage 10 mg/kg the drug reached effective therapeutic concentrations in renal tissue for treating fungal infections. In the Caco-2 transport studies, GMO cubosomes resulted in a significantly larger amount of AmB being transported into Caco-2 cells, via both clathrin- and caveolae-mediated endocytosis, but not macropinocytosis. These results suggest that GMO cubosomes, as lipid nanovectors, could facilitate the oral delivery of AmB.Keywords: glyceryl monoolein cubosomes, oral delivery, amphotericin B, antifungal activity, absorption mechanism
format	article
author	Yang Z Chen M Yang M Chen J Fang W Xu P
author_facet	Yang Z Chen M Yang M Chen J Fang W Xu P
author_sort	Yang Z
title	Evaluating the potential of cubosomal nanoparticles for oral delivery of amphotericin B in treating fungal infection
title_short	Evaluating the potential of cubosomal nanoparticles for oral delivery of amphotericin B in treating fungal infection
title_full	Evaluating the potential of cubosomal nanoparticles for oral delivery of amphotericin B in treating fungal infection
title_fullStr	Evaluating the potential of cubosomal nanoparticles for oral delivery of amphotericin B in treating fungal infection
title_full_unstemmed	Evaluating the potential of cubosomal nanoparticles for oral delivery of amphotericin B in treating fungal infection
title_sort	evaluating the potential of cubosomal nanoparticles for oral delivery of amphotericin b in treating fungal infection
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/3c3b818ad0c44b7585e6f76f3af76acd
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Evaluating the potential of cubosomal nanoparticles for oral delivery of amphotericin B in treating fungal infection

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