Optogenetic Neuronal Silencing in Drosophila during Visual Processing

Abstract Optogenetic channels and ion pumps have become indispensable tools in neuroscience to manipulate neuronal activity and thus to establish synaptic connectivity and behavioral causality. Inhibitory channels are particularly advantageous to explore signal processing in neural circuits since th...

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Autores principales:	Alex S. Mauss, Christian Busch, Alexander Borst
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3c40be9c0bca457c91cb1f00dc86aa51
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spelling	oai:doaj.org-article:3c40be9c0bca457c91cb1f00dc86aa512021-12-02T11:53:08ZOptogenetic Neuronal Silencing in Drosophila during Visual Processing10.1038/s41598-017-14076-72045-2322https://doaj.org/article/3c40be9c0bca457c91cb1f00dc86aa512017-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-14076-7https://doaj.org/toc/2045-2322Abstract Optogenetic channels and ion pumps have become indispensable tools in neuroscience to manipulate neuronal activity and thus to establish synaptic connectivity and behavioral causality. Inhibitory channels are particularly advantageous to explore signal processing in neural circuits since they permit the functional removal of selected neurons on a trial-by-trial basis. However, applying these tools to study the visual system poses a considerable challenge because the illumination required for their activation usually also stimulates photoreceptors substantially, precluding the simultaneous probing of visual responses. Here, we explore the utility of the recently discovered anion channelrhodopsins GtACR1 and GtACR2 for application in the visual system of Drosophila. We first characterized their properties using a larval crawling assay. We further obtained whole-cell recordings from cells expressing GtACR1, which mediated strong and light-sensitive photocurrents. Finally, using physiological recordings and a behavioral readout, we demonstrate that GtACR1 enables the fast and reversible silencing of genetically targeted neurons within circuits engaged in visual processing.Alex S. MaussChristian BuschAlexander BorstNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alex S. Mauss Christian Busch Alexander Borst Optogenetic Neuronal Silencing in Drosophila during Visual Processing
description	Abstract Optogenetic channels and ion pumps have become indispensable tools in neuroscience to manipulate neuronal activity and thus to establish synaptic connectivity and behavioral causality. Inhibitory channels are particularly advantageous to explore signal processing in neural circuits since they permit the functional removal of selected neurons on a trial-by-trial basis. However, applying these tools to study the visual system poses a considerable challenge because the illumination required for their activation usually also stimulates photoreceptors substantially, precluding the simultaneous probing of visual responses. Here, we explore the utility of the recently discovered anion channelrhodopsins GtACR1 and GtACR2 for application in the visual system of Drosophila. We first characterized their properties using a larval crawling assay. We further obtained whole-cell recordings from cells expressing GtACR1, which mediated strong and light-sensitive photocurrents. Finally, using physiological recordings and a behavioral readout, we demonstrate that GtACR1 enables the fast and reversible silencing of genetically targeted neurons within circuits engaged in visual processing.
format	article
author	Alex S. Mauss Christian Busch Alexander Borst
author_facet	Alex S. Mauss Christian Busch Alexander Borst
author_sort	Alex S. Mauss
title	Optogenetic Neuronal Silencing in Drosophila during Visual Processing
title_short	Optogenetic Neuronal Silencing in Drosophila during Visual Processing
title_full	Optogenetic Neuronal Silencing in Drosophila during Visual Processing
title_fullStr	Optogenetic Neuronal Silencing in Drosophila during Visual Processing
title_full_unstemmed	Optogenetic Neuronal Silencing in Drosophila during Visual Processing
title_sort	optogenetic neuronal silencing in drosophila during visual processing
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3c40be9c0bca457c91cb1f00dc86aa51
work_keys_str_mv	AT alexsmauss optogeneticneuronalsilencingindrosophiladuringvisualprocessing AT christianbusch optogeneticneuronalsilencingindrosophiladuringvisualprocessing AT alexanderborst optogeneticneuronalsilencingindrosophiladuringvisualprocessing
_version_	1718394861905772544

Optogenetic Neuronal Silencing in Drosophila during Visual Processing

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