Seed Paternity Analysis Using SSR Markers to Assess Successful Pollen Donors in Mixed Olive Orchards

The olive tree (<i>Olea europaea</i> L.) is a wind-pollinated crop that exhibits an extreme alternate bearing habit. To improve fruit set, several methods have been used to determine the most successful compatible combinations of cultivars. In this study, priority is given to seed patern...

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Autores principales:	Gabriela Vuletin Selak, Alenka Baruca Arbeiter, Julián Cuevas, Slavko Perica, Petar Pujic, Marina Raboteg Božiković, Dunja Bandelj
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Olea europaea</i> L. cultivar ‘Oblica’ microsatellites seed paternity assignment cross-compatibility self-incompatibility Botany QK1-989
Acceso en línea:	https://doaj.org/article/3c44cc023a6c47a1857381ce2b0e79c9
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id	oai:doaj.org-article:3c44cc023a6c47a1857381ce2b0e79c9
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spelling	oai:doaj.org-article:3c44cc023a6c47a1857381ce2b0e79c92021-11-25T18:45:44ZSeed Paternity Analysis Using SSR Markers to Assess Successful Pollen Donors in Mixed Olive Orchards10.3390/plants101123562223-7747https://doaj.org/article/3c44cc023a6c47a1857381ce2b0e79c92021-10-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2356https://doaj.org/toc/2223-7747The olive tree (<i>Olea europaea</i> L.) is a wind-pollinated crop that exhibits an extreme alternate bearing habit. To improve fruit set, several methods have been used to determine the most successful compatible combinations of cultivars. In this study, priority is given to seed paternity analysis based on simple sequence repeats (SSRs), microsatellite markers used for the identification of potential pollen donors of cultivar ‘Oblica’ in a mixed olive orchard during two consecutive years. Seven microsatellite primers were successfully used to examine the paternity of olive embryos from ‘Oblica’ mother trees. Embryos were considered as a product of self-fertilization if only maternal alleles were present, but not a single case of self-fertilization was found among all the embryos analyzed. Two dominant pollen donors were not the closest nor the cultivars with the highest number of trees in the orchard, suggesting that cross-compatibility may have a key role in determining pollen donor success. In our earlier studies, pollen tube growth and fertilization success correlated with fruit set when controlled crosses between cultivars were performed; however, some discrepancy might appear compared to paternity analyses when mother trees have a free choice among different pollen sources from cultivars growing in their surroundings.Gabriela Vuletin SelakAlenka Baruca ArbeiterJulián CuevasSlavko PericaPetar PujicMarina Raboteg BožikovićDunja BandeljMDPI AGarticle<i>Olea europaea</i> L.cultivar ‘Oblica’microsatellitesseed paternity assignmentcross-compatibilityself-incompatibilityBotanyQK1-989ENPlants, Vol 10, Iss 2356, p 2356 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Olea europaea</i> L. cultivar ‘Oblica’ microsatellites seed paternity assignment cross-compatibility self-incompatibility Botany QK1-989
spellingShingle	<i>Olea europaea</i> L. cultivar ‘Oblica’ microsatellites seed paternity assignment cross-compatibility self-incompatibility Botany QK1-989 Gabriela Vuletin Selak Alenka Baruca Arbeiter Julián Cuevas Slavko Perica Petar Pujic Marina Raboteg Božiković Dunja Bandelj Seed Paternity Analysis Using SSR Markers to Assess Successful Pollen Donors in Mixed Olive Orchards
description	The olive tree (<i>Olea europaea</i> L.) is a wind-pollinated crop that exhibits an extreme alternate bearing habit. To improve fruit set, several methods have been used to determine the most successful compatible combinations of cultivars. In this study, priority is given to seed paternity analysis based on simple sequence repeats (SSRs), microsatellite markers used for the identification of potential pollen donors of cultivar ‘Oblica’ in a mixed olive orchard during two consecutive years. Seven microsatellite primers were successfully used to examine the paternity of olive embryos from ‘Oblica’ mother trees. Embryos were considered as a product of self-fertilization if only maternal alleles were present, but not a single case of self-fertilization was found among all the embryos analyzed. Two dominant pollen donors were not the closest nor the cultivars with the highest number of trees in the orchard, suggesting that cross-compatibility may have a key role in determining pollen donor success. In our earlier studies, pollen tube growth and fertilization success correlated with fruit set when controlled crosses between cultivars were performed; however, some discrepancy might appear compared to paternity analyses when mother trees have a free choice among different pollen sources from cultivars growing in their surroundings.
format	article
author	Gabriela Vuletin Selak Alenka Baruca Arbeiter Julián Cuevas Slavko Perica Petar Pujic Marina Raboteg Božiković Dunja Bandelj
author_facet	Gabriela Vuletin Selak Alenka Baruca Arbeiter Julián Cuevas Slavko Perica Petar Pujic Marina Raboteg Božiković Dunja Bandelj
author_sort	Gabriela Vuletin Selak
title	Seed Paternity Analysis Using SSR Markers to Assess Successful Pollen Donors in Mixed Olive Orchards
title_short	Seed Paternity Analysis Using SSR Markers to Assess Successful Pollen Donors in Mixed Olive Orchards
title_full	Seed Paternity Analysis Using SSR Markers to Assess Successful Pollen Donors in Mixed Olive Orchards
title_fullStr	Seed Paternity Analysis Using SSR Markers to Assess Successful Pollen Donors in Mixed Olive Orchards
title_full_unstemmed	Seed Paternity Analysis Using SSR Markers to Assess Successful Pollen Donors in Mixed Olive Orchards
title_sort	seed paternity analysis using ssr markers to assess successful pollen donors in mixed olive orchards
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3c44cc023a6c47a1857381ce2b0e79c9
work_keys_str_mv	AT gabrielavuletinselak seedpaternityanalysisusingssrmarkerstoassesssuccessfulpollendonorsinmixedoliveorchards AT alenkabarucaarbeiter seedpaternityanalysisusingssrmarkerstoassesssuccessfulpollendonorsinmixedoliveorchards AT juliancuevas seedpaternityanalysisusingssrmarkerstoassesssuccessfulpollendonorsinmixedoliveorchards AT slavkoperica seedpaternityanalysisusingssrmarkerstoassesssuccessfulpollendonorsinmixedoliveorchards AT petarpujic seedpaternityanalysisusingssrmarkerstoassesssuccessfulpollendonorsinmixedoliveorchards AT marinarabotegbozikovic seedpaternityanalysisusingssrmarkerstoassesssuccessfulpollendonorsinmixedoliveorchards AT dunjabandelj seedpaternityanalysisusingssrmarkerstoassesssuccessfulpollendonorsinmixedoliveorchards
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Seed Paternity Analysis Using SSR Markers to Assess Successful Pollen Donors in Mixed Olive Orchards

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