Dynamics of plasma membrane surface related to the release of extracellular vesicles by mesenchymal stem cells in culture

Abstract Extracellular vesicles (exosomes and shedding vesicles) released by mesenchymal stem cells (MSCs) are regarded as a storable, cell-free alternative with comparable therapeutic potential to their parent cells. Shedding vesicles originate as bulges on the cell surface but little is known abou...

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Autores principales:	Santiago Casado, Maria del Val Toledo Lobo, Carlos Luis Paíno
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3c48ffe95edb467797a80e31c368e4a0
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spelling	oai:doaj.org-article:3c48ffe95edb467797a80e31c368e4a02021-12-02T16:06:29ZDynamics of plasma membrane surface related to the release of extracellular vesicles by mesenchymal stem cells in culture10.1038/s41598-017-07265-x2045-2322https://doaj.org/article/3c48ffe95edb467797a80e31c368e4a02017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07265-xhttps://doaj.org/toc/2045-2322Abstract Extracellular vesicles (exosomes and shedding vesicles) released by mesenchymal stem cells (MSCs) are regarded as a storable, cell-free alternative with comparable therapeutic potential to their parent cells. Shedding vesicles originate as bulges on the cell surface but little is known about their turnover or how their formation can be stimulated. We have used atomic force microscopy (AFM) to follow the formation dynamics of bulges in living adipose tissue-derived MSCs. AFM images showed that, in general, MSCs present hundreds of nanosized protrusions on their surface with life spans of 10–20 min. Scanning electron microscopy confirmed those images and showed that bulges are also formed on filamentous processes. Extracellular vesicles deposited on the culture surface have comparable sizes to those of bulges showing up on the cell surface. The amount of protrusions on cells treated with progesterone or PDGF-BB, two treatments that stimulate the secretion of extracellular vesicles in MSCs, was evaluated by AFM. Measurements of the cross-area at 50 nm over the cell surface provided estimates of the amount of protrusions and showed that these values increased with the stimulating treatments. Our study suggests that shedding vesicles constitute a large population of the extracellular vesicle pool.Santiago CasadoMaria del Val Toledo LoboCarlos Luis PaínoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Santiago Casado Maria del Val Toledo Lobo Carlos Luis Paíno Dynamics of plasma membrane surface related to the release of extracellular vesicles by mesenchymal stem cells in culture
description	Abstract Extracellular vesicles (exosomes and shedding vesicles) released by mesenchymal stem cells (MSCs) are regarded as a storable, cell-free alternative with comparable therapeutic potential to their parent cells. Shedding vesicles originate as bulges on the cell surface but little is known about their turnover or how their formation can be stimulated. We have used atomic force microscopy (AFM) to follow the formation dynamics of bulges in living adipose tissue-derived MSCs. AFM images showed that, in general, MSCs present hundreds of nanosized protrusions on their surface with life spans of 10–20 min. Scanning electron microscopy confirmed those images and showed that bulges are also formed on filamentous processes. Extracellular vesicles deposited on the culture surface have comparable sizes to those of bulges showing up on the cell surface. The amount of protrusions on cells treated with progesterone or PDGF-BB, two treatments that stimulate the secretion of extracellular vesicles in MSCs, was evaluated by AFM. Measurements of the cross-area at 50 nm over the cell surface provided estimates of the amount of protrusions and showed that these values increased with the stimulating treatments. Our study suggests that shedding vesicles constitute a large population of the extracellular vesicle pool.
format	article
author	Santiago Casado Maria del Val Toledo Lobo Carlos Luis Paíno
author_facet	Santiago Casado Maria del Val Toledo Lobo Carlos Luis Paíno
author_sort	Santiago Casado
title	Dynamics of plasma membrane surface related to the release of extracellular vesicles by mesenchymal stem cells in culture
title_short	Dynamics of plasma membrane surface related to the release of extracellular vesicles by mesenchymal stem cells in culture
title_full	Dynamics of plasma membrane surface related to the release of extracellular vesicles by mesenchymal stem cells in culture
title_fullStr	Dynamics of plasma membrane surface related to the release of extracellular vesicles by mesenchymal stem cells in culture
title_full_unstemmed	Dynamics of plasma membrane surface related to the release of extracellular vesicles by mesenchymal stem cells in culture
title_sort	dynamics of plasma membrane surface related to the release of extracellular vesicles by mesenchymal stem cells in culture
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3c48ffe95edb467797a80e31c368e4a0
work_keys_str_mv	AT santiagocasado dynamicsofplasmamembranesurfacerelatedtothereleaseofextracellularvesiclesbymesenchymalstemcellsinculture AT mariadelvaltoledolobo dynamicsofplasmamembranesurfacerelatedtothereleaseofextracellularvesiclesbymesenchymalstemcellsinculture AT carlosluispaino dynamicsofplasmamembranesurfacerelatedtothereleaseofextracellularvesiclesbymesenchymalstemcellsinculture
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Dynamics of plasma membrane surface related to the release of extracellular vesicles by mesenchymal stem cells in culture

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