Simple and Feasible Detection of Hepatitis B Virus via Combination of Multienzyme Isothermal Rapid Amplification and Lateral Flow Dipstick Strip

Hepatitis B virus infection is not only a huge burden in the field of social health but also a major public health problem that affects the lives and health of the people. Simple, rapid, feasible detection of HBV is critical for its prevention and spread, especially in the developing countries with...

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Autores principales:	Mao-Ling Sun, Hai-Yun Lai, Na-Yu Chong, Dong-Fan Liu, Zhen-Yi Zhang, Bo Pang, Jun Yao
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	hepatitis B virus multienzyme isothermal rapid amplification lateral flow strip detection nucleic acid Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3c4947f6c42a4e6bbc3fd0ca3ebc7934
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spelling	oai:doaj.org-article:3c4947f6c42a4e6bbc3fd0ca3ebc79342021-12-02T10:04:12ZSimple and Feasible Detection of Hepatitis B Virus via Combination of Multienzyme Isothermal Rapid Amplification and Lateral Flow Dipstick Strip2296-889X10.3389/fmolb.2021.763079https://doaj.org/article/3c4947f6c42a4e6bbc3fd0ca3ebc79342021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmolb.2021.763079/fullhttps://doaj.org/toc/2296-889XHepatitis B virus infection is not only a huge burden in the field of social health but also a major public health problem that affects the lives and health of the people. Simple, rapid, feasible detection of HBV is critical for its prevention and spread, especially in the developing countries with low-resource laboratories. To this end, we combined multienzyme isothermal rapid amplification (MIRA) and lateral flow dipstick (LFD) strip to detect HBV. A pair of primers targeting the conserved region of HBV genome was designed and used in MIRA-LFD assay. Our results found that the entire amplification of MIRA-LFD only takes 10 min at 37°C and the dilution of the amplification products was added in the LFD strip and observed by the naked eye after 10 min. The detection sensitivity of this method can reach 10 pg. The 45 clinical samples were detected by MIRA-LFD and real-time PCR. The accuracy rate of MIRA-LFD was 100%. Therefore, these characteristics of our newly developed MIRA-LFD assay make it particularly useful and suitable for detecting HBV in the resource-limited condition.Mao-Ling SunHai-Yun LaiNa-Yu ChongDong-Fan LiuZhen-Yi ZhangBo PangJun YaoFrontiers Media S.A.articlehepatitis B virusmultienzyme isothermal rapid amplificationlateral flow stripdetectionnucleic acidBiology (General)QH301-705.5ENFrontiers in Molecular Biosciences, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hepatitis B virus multienzyme isothermal rapid amplification lateral flow strip detection nucleic acid Biology (General) QH301-705.5
spellingShingle	hepatitis B virus multienzyme isothermal rapid amplification lateral flow strip detection nucleic acid Biology (General) QH301-705.5 Mao-Ling Sun Hai-Yun Lai Na-Yu Chong Dong-Fan Liu Zhen-Yi Zhang Bo Pang Jun Yao Simple and Feasible Detection of Hepatitis B Virus via Combination of Multienzyme Isothermal Rapid Amplification and Lateral Flow Dipstick Strip
description	Hepatitis B virus infection is not only a huge burden in the field of social health but also a major public health problem that affects the lives and health of the people. Simple, rapid, feasible detection of HBV is critical for its prevention and spread, especially in the developing countries with low-resource laboratories. To this end, we combined multienzyme isothermal rapid amplification (MIRA) and lateral flow dipstick (LFD) strip to detect HBV. A pair of primers targeting the conserved region of HBV genome was designed and used in MIRA-LFD assay. Our results found that the entire amplification of MIRA-LFD only takes 10 min at 37°C and the dilution of the amplification products was added in the LFD strip and observed by the naked eye after 10 min. The detection sensitivity of this method can reach 10 pg. The 45 clinical samples were detected by MIRA-LFD and real-time PCR. The accuracy rate of MIRA-LFD was 100%. Therefore, these characteristics of our newly developed MIRA-LFD assay make it particularly useful and suitable for detecting HBV in the resource-limited condition.
format	article
author	Mao-Ling Sun Hai-Yun Lai Na-Yu Chong Dong-Fan Liu Zhen-Yi Zhang Bo Pang Jun Yao
author_facet	Mao-Ling Sun Hai-Yun Lai Na-Yu Chong Dong-Fan Liu Zhen-Yi Zhang Bo Pang Jun Yao
author_sort	Mao-Ling Sun
title	Simple and Feasible Detection of Hepatitis B Virus via Combination of Multienzyme Isothermal Rapid Amplification and Lateral Flow Dipstick Strip
title_short	Simple and Feasible Detection of Hepatitis B Virus via Combination of Multienzyme Isothermal Rapid Amplification and Lateral Flow Dipstick Strip
title_full	Simple and Feasible Detection of Hepatitis B Virus via Combination of Multienzyme Isothermal Rapid Amplification and Lateral Flow Dipstick Strip
title_fullStr	Simple and Feasible Detection of Hepatitis B Virus via Combination of Multienzyme Isothermal Rapid Amplification and Lateral Flow Dipstick Strip
title_full_unstemmed	Simple and Feasible Detection of Hepatitis B Virus via Combination of Multienzyme Isothermal Rapid Amplification and Lateral Flow Dipstick Strip
title_sort	simple and feasible detection of hepatitis b virus via combination of multienzyme isothermal rapid amplification and lateral flow dipstick strip
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/3c4947f6c42a4e6bbc3fd0ca3ebc7934
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Simple and Feasible Detection of Hepatitis B Virus via Combination of Multienzyme Isothermal Rapid Amplification and Lateral Flow Dipstick Strip

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