Immunochromatographic paper sensor for ultrasensitive colorimetric detection of cadmium

A novel and highly sensitive immunochromatographic strip based on a monoclonal antibody (3A9) was developed for the detection of cadmium in tap water. The 50% inhibition concentration of the antibody, which showed no cross-reactivity with other heavy metal ions, was 0.45 ng/mL and it recognized Cd(I...

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Autores principales: Shanshan Song, Shuzhen Zou, Jianping Zhu, Liqiang Liu, Hua Kuang
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Lenguaje:EN
Publicado: Taylor & Francis Group 2018
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Acceso en línea:https://doaj.org/article/3c51fc5cf87b4d378d3a89581d87e974
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spelling oai:doaj.org-article:3c51fc5cf87b4d378d3a89581d87e9742021-11-26T11:19:46ZImmunochromatographic paper sensor for ultrasensitive colorimetric detection of cadmium0954-01051465-344310.1080/09540105.2017.1354358https://doaj.org/article/3c51fc5cf87b4d378d3a89581d87e9742018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2017.1354358https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443A novel and highly sensitive immunochromatographic strip based on a monoclonal antibody (3A9) was developed for the detection of cadmium in tap water. The 50% inhibition concentration of the antibody, which showed no cross-reactivity with other heavy metal ions, was 0.45 ng/mL and it recognized Cd(II)–1-(4-isothiocyanobenzyl) ethylenediamine-N,N,N′,N′-tetraacetic acid (EDTA) (Cd(II)–ITCBE) and not metal-free ITCBE and EDTA. The cutoff value for semi-quantitative detection of the strip was 5 ng/mL and the lower limit of detection for quantitative detection was 0.2 ng/mL using a scanning reader. The percent recovery ranged from 107.6% to 132% in tap water samples.Shanshan SongShuzhen ZouJianping ZhuLiqiang LiuHua KuangTaylor & Francis Grouparticlecadmiumimmunochromatographic stripmonoclonal antibodygold-nanoparticle (gnp)Agriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 3-13 (2018)
institution DOAJ
collection DOAJ
language EN
topic cadmium
immunochromatographic strip
monoclonal antibody
gold-nanoparticle (gnp)
Agriculture (General)
S1-972
Immunologic diseases. Allergy
RC581-607
spellingShingle cadmium
immunochromatographic strip
monoclonal antibody
gold-nanoparticle (gnp)
Agriculture (General)
S1-972
Immunologic diseases. Allergy
RC581-607
Shanshan Song
Shuzhen Zou
Jianping Zhu
Liqiang Liu
Hua Kuang
Immunochromatographic paper sensor for ultrasensitive colorimetric detection of cadmium
description A novel and highly sensitive immunochromatographic strip based on a monoclonal antibody (3A9) was developed for the detection of cadmium in tap water. The 50% inhibition concentration of the antibody, which showed no cross-reactivity with other heavy metal ions, was 0.45 ng/mL and it recognized Cd(II)–1-(4-isothiocyanobenzyl) ethylenediamine-N,N,N′,N′-tetraacetic acid (EDTA) (Cd(II)–ITCBE) and not metal-free ITCBE and EDTA. The cutoff value for semi-quantitative detection of the strip was 5 ng/mL and the lower limit of detection for quantitative detection was 0.2 ng/mL using a scanning reader. The percent recovery ranged from 107.6% to 132% in tap water samples.
format article
author Shanshan Song
Shuzhen Zou
Jianping Zhu
Liqiang Liu
Hua Kuang
author_facet Shanshan Song
Shuzhen Zou
Jianping Zhu
Liqiang Liu
Hua Kuang
author_sort Shanshan Song
title Immunochromatographic paper sensor for ultrasensitive colorimetric detection of cadmium
title_short Immunochromatographic paper sensor for ultrasensitive colorimetric detection of cadmium
title_full Immunochromatographic paper sensor for ultrasensitive colorimetric detection of cadmium
title_fullStr Immunochromatographic paper sensor for ultrasensitive colorimetric detection of cadmium
title_full_unstemmed Immunochromatographic paper sensor for ultrasensitive colorimetric detection of cadmium
title_sort immunochromatographic paper sensor for ultrasensitive colorimetric detection of cadmium
publisher Taylor & Francis Group
publishDate 2018
url https://doaj.org/article/3c51fc5cf87b4d378d3a89581d87e974
work_keys_str_mv AT shanshansong immunochromatographicpapersensorforultrasensitivecolorimetricdetectionofcadmium
AT shuzhenzou immunochromatographicpapersensorforultrasensitivecolorimetricdetectionofcadmium
AT jianpingzhu immunochromatographicpapersensorforultrasensitivecolorimetricdetectionofcadmium
AT liqiangliu immunochromatographicpapersensorforultrasensitivecolorimetricdetectionofcadmium
AT huakuang immunochromatographicpapersensorforultrasensitivecolorimetricdetectionofcadmium
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