Preservation of Features of Anatomical Polymorphism of Deschampsia antarctica É. Desv. (Poaceae) During In Vitro Clonal Reproduction

We studied the anatomical features of the leaf structure of Deschampsia antarctica É. Desv. (Poaceae) obtained from seeds collected from different habitats of the maritime Antarctic. These plants have been maintained in culture in vitro for more than 7 years. The plants include stable chromosome for...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nataliia Nuzhyna, Viktor Kunakh, Oksana Poronnik, Ivan Parnikoza
Formato:	article
Lenguaje:	EN
Publicado:	Polish Botanical Society 2021
Materias:	antarctic hairgrass diﬀerent growth habitats leaf structure genetic diversity adaptation Agriculture (General) S1-972
Acceso en línea:	https://doaj.org/article/3c5417849f2648cb97e45af440d0b02f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3c5417849f2648cb97e45af440d0b02f
record_format	dspace
spelling	oai:doaj.org-article:3c5417849f2648cb97e45af440d0b02f2021-12-01T23:00:03ZPreservation of Features of Anatomical Polymorphism of Deschampsia antarctica É. Desv. (Poaceae) During In Vitro Clonal Reproduction2300-357X10.5586/aa.7416https://doaj.org/article/3c5417849f2648cb97e45af440d0b02f2021-12-01T00:00:00Zhttps://pbsociety.org.pl/journals/index.php/aa/article/view/8902https://doaj.org/toc/2300-357XWe studied the anatomical features of the leaf structure of Deschampsia antarctica É. Desv. (Poaceae) obtained from seeds collected from different habitats of the maritime Antarctic. These plants have been maintained in culture in vitro for more than 7 years. The plants include stable chromosome forms comprising diploids (2n = 26), a diploid plant with B-chromosomes (2n = 26 + 0–3B), a triploid with rearranged chromosome morphology, and myxoploids with different ratios of diploid and nondiploid cells in the root meristem. The D. antarctica plants that were studied generally had a similar anatomical structure in culture in vitro and in nature. At the same time, plants of different cultivated genotypes also displayed their own leaf structure features. In particular, qualitative features included asymmetric four-ribs and five-rib leaves instead of typical three-rib leaves for some genotypes, some individuals characterized by the presence of unicellular nonglandular pointed trichomes on an adaxial side, differences in vascular bundle sheath, and other features. No clear dependence of the anatomical structural features on the chromosomal status of the studied genotypes was evident. At the same time, differences were evident in traits that included the number of stomata, thickness of the outer cell walls of the epidermis, bundles sheath state, number of leaf ribs, and the persistent presence of trichomes under prolonged in vitro cloning. The findings indicate that D. antarctica plants collected from different locations of the maritime Antarctic for long-term cultivation in vitro under standard cultivation conditions retain the anatomical characteristics of the wild plants. The polymorphism of in vitro-cultivated plants is not related to the polymorphism of their karyotype, but is apparently due to the heterogeneity of the original plants and possibly the epigenetic fixation of a number of anatomical features produced in the natural microhabitats of D. antarctica populations.Nataliia NuzhynaViktor KunakhOksana PoronnikIvan ParnikozaPolish Botanical Societyarticleantarctic hairgrassdiﬀerent growth habitatsleaf structuregenetic diversityadaptationAgriculture (General)S1-972ENActa Agrobotanica, Vol 74, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antarctic hairgrass diﬀerent growth habitats leaf structure genetic diversity adaptation Agriculture (General) S1-972
spellingShingle	antarctic hairgrass diﬀerent growth habitats leaf structure genetic diversity adaptation Agriculture (General) S1-972 Nataliia Nuzhyna Viktor Kunakh Oksana Poronnik Ivan Parnikoza Preservation of Features of Anatomical Polymorphism of Deschampsia antarctica É. Desv. (Poaceae) During In Vitro Clonal Reproduction
description	We studied the anatomical features of the leaf structure of Deschampsia antarctica É. Desv. (Poaceae) obtained from seeds collected from different habitats of the maritime Antarctic. These plants have been maintained in culture in vitro for more than 7 years. The plants include stable chromosome forms comprising diploids (2n = 26), a diploid plant with B-chromosomes (2n = 26 + 0–3B), a triploid with rearranged chromosome morphology, and myxoploids with different ratios of diploid and nondiploid cells in the root meristem. The D. antarctica plants that were studied generally had a similar anatomical structure in culture in vitro and in nature. At the same time, plants of different cultivated genotypes also displayed their own leaf structure features. In particular, qualitative features included asymmetric four-ribs and five-rib leaves instead of typical three-rib leaves for some genotypes, some individuals characterized by the presence of unicellular nonglandular pointed trichomes on an adaxial side, differences in vascular bundle sheath, and other features. No clear dependence of the anatomical structural features on the chromosomal status of the studied genotypes was evident. At the same time, differences were evident in traits that included the number of stomata, thickness of the outer cell walls of the epidermis, bundles sheath state, number of leaf ribs, and the persistent presence of trichomes under prolonged in vitro cloning. The findings indicate that D. antarctica plants collected from different locations of the maritime Antarctic for long-term cultivation in vitro under standard cultivation conditions retain the anatomical characteristics of the wild plants. The polymorphism of in vitro-cultivated plants is not related to the polymorphism of their karyotype, but is apparently due to the heterogeneity of the original plants and possibly the epigenetic fixation of a number of anatomical features produced in the natural microhabitats of D. antarctica populations.
format	article
author	Nataliia Nuzhyna Viktor Kunakh Oksana Poronnik Ivan Parnikoza
author_facet	Nataliia Nuzhyna Viktor Kunakh Oksana Poronnik Ivan Parnikoza
author_sort	Nataliia Nuzhyna
title	Preservation of Features of Anatomical Polymorphism of Deschampsia antarctica É. Desv. (Poaceae) During In Vitro Clonal Reproduction
title_short	Preservation of Features of Anatomical Polymorphism of Deschampsia antarctica É. Desv. (Poaceae) During In Vitro Clonal Reproduction
title_full	Preservation of Features of Anatomical Polymorphism of Deschampsia antarctica É. Desv. (Poaceae) During In Vitro Clonal Reproduction
title_fullStr	Preservation of Features of Anatomical Polymorphism of Deschampsia antarctica É. Desv. (Poaceae) During In Vitro Clonal Reproduction
title_full_unstemmed	Preservation of Features of Anatomical Polymorphism of Deschampsia antarctica É. Desv. (Poaceae) During In Vitro Clonal Reproduction
title_sort	preservation of features of anatomical polymorphism of deschampsia antarctica é. desv. (poaceae) during in vitro clonal reproduction
publisher	Polish Botanical Society
publishDate	2021
url	https://doaj.org/article/3c5417849f2648cb97e45af440d0b02f
work_keys_str_mv	AT nataliianuzhyna preservationoffeaturesofanatomicalpolymorphismofdeschampsiaantarcticaedesvpoaceaeduringinvitroclonalreproduction AT viktorkunakh preservationoffeaturesofanatomicalpolymorphismofdeschampsiaantarcticaedesvpoaceaeduringinvitroclonalreproduction AT oksanaporonnik preservationoffeaturesofanatomicalpolymorphismofdeschampsiaantarcticaedesvpoaceaeduringinvitroclonalreproduction AT ivanparnikoza preservationoffeaturesofanatomicalpolymorphismofdeschampsiaantarcticaedesvpoaceaeduringinvitroclonalreproduction
_version_	1718404033725595648

Preservation of Features of Anatomical Polymorphism of Deschampsia antarctica É. Desv. (Poaceae) During In Vitro Clonal Reproduction

Ejemplares similares